Angiotensin II (Ang II) (Human) ELISA Kit

Supplier: Abcam

AB285306-96
ABCAAB285306-96EA 1430 EUR
ABCAAB285306-96
Angiotensin II (Ang II) (Human) ELISA Kit
Assays ELISAs

Angiotensin II ELISA kit is a competitive ELISA assay for the quantitative measurement of Ang II in Human serum, plasma, tissue homogenates and other biological fluids.


  • Easy, convenient, and time-saving method to assay for human p53
  • The minimum detectable dose of p53 is typically less than 2 µg per sample


Angiotensin II acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. The microtiter plate provided in this kit has been pre-coated with the target antigen. During the reaction, the target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target.


The unbound sample or excess conjugate are washed from the plate. HRP-Streptavidin (SABC) is added and incubated. Then TMB substrate solution is added to each well. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is measure at 450 nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.


This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF,BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-γ, Leptin (OB), MCP-1, MDC, MIP-1α, MIP-1 β, MIP-1δ, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF.


Caution:

All products are FOR RESEARCH USE ONLY.   PRODUCTS ARE NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES.  Customer is granted a limited use non-exclusive licence for its own internal research.  Any control manufacturer supplies with a Product is limited to use only to validate the relevant Product.  By using the Product, Customers agree not to attempt to reverse engineer or otherwise perform any compositional, structural, functional or other analyses directed to learning the methodology, formulae, sequences, processes, make-up or production of any of the Products or portions thereof.


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