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You Searched For: Enzyme Assays

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.


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Product Image-AATB13456

Amplite® Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*

Catalog Number: (AATB13456)
Supplier: AAT BIOQUEST
Description: Our Amplite® Fluorometric 20S Proteasome Assay Kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex either in cultured cells or cell lysates.
UOM: 1 * 100 Tests
Product Image-AATB11551

Amplite® Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*

Catalog Number: (AATB11551)
Supplier: AAT BIOQUEST
Description: Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.
UOM: 1 * 500 Tests
Product Image-AATB10087

Amplite® Fluorimetric α-Ketoglutarate Quantitation Kit

Catalog Number: (AATB10087)
Supplier: AAT BIOQUEST
Description: Alpha-ketoglutarate (α-ketoglutarate) is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism.
UOM: 1 * 200 Tests
Product Image-AATB11105

Amplite® Fluorimetric Protein Quantitation Kit *Orange Fluorescence*

Catalog Number: (AATB11105)
Supplier: AAT BIOQUEST
Description: Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.
UOM: 1 * 500 Tests
Product Image-786-440

Peroxide assay kit, PEROXsay™

Supplier: G-Biosciences
Description: PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe²⁺+) ions to ferric (Fe³⁺) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the assay allows the ferric (Fe³⁺) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.

Product Image-786-431

SAM Methyltransferase assay, SAMfluoro™

Catalog Number: (786-431)
Supplier: G-Biosciences
Description: The SAMfluoro™ assay is a fluorescent, continuous enzyme coupled assay that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.
UOM: 1 * 100 Assays
Product Image-PIER16159

Pierce™ Gaussia Luciferase Flash Assay Kit

Supplier: Thermo Fisher Scientific
Description: The Thermo Scientific Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.

Product Image-PIER88317

Serine hydrolase probes, ActivX™ FP

Supplier: Thermo Fisher Scientific
Description: The ActivX® FP serine hydrolase probes enable selective labeling and enrichment of active serine hydrolases. Fluorophosphonate (FP) probes specifically label serines of enzymatically active serine hydrolases. FP probes are also effective for screening small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins. Depending on the active-site probe tag group and the enzyme class to be profiled, active site-labeled enzymes are detected and quantified by Western blot, fluorescent gel imaging or mass spectrometry.

Product Image-OZBIGO10001

ONPG assay kit

Catalog Number: (OZBIGO10001)
Supplier: OZ BIOSCIENCES
Description: ONPG assay kit is ideal to quantify high expression level of beta-Gal.
UOM: 1 * 1 KIT
Product Image-PIER16182

Gaussia-firefly luciferase dual assay kit, Pierce™, Thermo Scientific

Supplier: Thermo Fisher Scientific
Description: The Thermo Scientific Pierce Gaussia-Firefly Luciferase dual assay kit provides the necessary reagents to simultaneously detect intracellular Gaussia and red firefly luciferase activity in mammalian whole cell lysates.

Product Image-AATB13520

MMP Green™ Substrate

Supplier: AAT BIOQUEST
Description: The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.

Product Image-AATB40006

Amplite® Cholesterol Quantitation Kit

Catalog Number: (AATB40006)
Supplier: AAT BIOQUEST
Description: Cholesterol is required to build and maintain cell membranes.
UOM: 1 * 200 Tests
Product Image-AATB13804

Amplite® Fluorimetric Glucose-6-Phosphate Assay Kit

Catalog Number: (AATB13804)
Supplier: AAT BIOQUEST
Description: Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells.
UOM: 1 * 200 Tests
Product Image-AATB12536

Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

Supplier: AAT BIOQUEST
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Product Image-AATB12532

Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

Supplier: AAT BIOQUEST
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Product Image-AATB11541

Amplite® Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*

Catalog Number: (AATB11541)
Supplier: AAT BIOQUEST
Description: Horseradish Peroxidase (HRP) is a small molecule (MW ~40 KD) that is widely used in a variety of biological detections.
UOM: 1 * 1.000 Tests
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
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