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You Searched For: Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.


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Product Image-PIER35071

Fast western blot kit, SuperSignal™ West Dura, Pierce™

Supplier: Thermo Fisher Scientific
Description: The Fast Western Blot Kits, SuperSignal™ West Dura provide reagents and protocol for reliable and rapid, 1 hour chemiluminescent Western blotting at femtogram levels of sensitivity. These complete Fast Western Blot Kits requires minimal hands on time and yield results comparable to classical Western blotting with SuperSignal™ West Dura Chemiluminescent Substrate. This substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse or rabbit primary antibody.

Image Unavailable-17-6003-18

Rehydration reagent, DeStreak

Supplier: Cytiva
Description: The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7,0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.

Product Image-RPN3682

Nucleic acid labelling and detection system, AlkPhos Direct™

Supplier: Cytiva
Description: AlkPhos Direct™ Labelling and detection systems are based on the rapid, direct labelling of DNA or RNA probes with thermostable alkaline phosphatase. AlkPhos Direct™ combines the convenience of direct enzyme labelling (no blocking or antibody stages) with those of alkaline phosphatase detection (long light output and high sensitivity).

Product Image-RPN3006

Amersham™ ECL Gold Hybridization Buffer, Cytiva

Catalog Number: (RPN3006)
Supplier: Cytiva
Description: ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
UOM: 1 * 1 items
Image Unavailable-RPN5785

Substrate for Western blotting, ECF™

Catalog Number: (RPN5785)
Supplier: Cytiva
Description: ECF™ substrate for Western blotting
UOM: 1 * 1 items
Product Image-RPN3005

Amersham ECL Direct Labeling System, Cytiva

Catalog Number: (RPN3005)
Supplier: Cytiva
Description: ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
UOM: 1 * 1 items
Product Image-RPN2109

Amersham™ ECL™ Western Blotting Detection Reagent, Cytivia

Supplier: Cytiva
Description: Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.

MSDS Certificates

Product Image-28-9042-49

Cy™ fluorescent amidites

Supplier: Cytiva
Description: Cy™ fluorescent amidites for incorporation of Cy3, Cy3,5, Cy5, Cy5,5 fluorescent dyes at any position in an oligonucleotide during synthesis.

Product Image-RPN3243

Western blotting detection reagent, Amersham™ ECL™ start

Supplier: Cytiva
Description: Chemiluminescence detection reagent for detection of high to medium abundant proteins.

Product Image-RPN2235

Western blotting detection reagent, Amersham™ ECL Select™

Catalog Number: (RPN2235)
Supplier: Cytiva
Description: Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection.
UOM: 1 * 1 KIT

MSDS Certificates


Product Image-PA15401

Fluorescent labelling reagents, CypHer5E NHS Ester

Supplier: Cytiva
Description: CypHer5E is a red-excitable, pH-sensitive cyanine dye derivative that is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH.

Product Image-RPN2232

Western blotting detection reagent, Amersham™ ECL™ Prime

Supplier: Cytiva
Description: Amersham™ ECL™ Prime is a highly sensitive Western blotting detection reagent characterised by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Amersham™ ECL™ Prime delivers a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. The high performance of Amersham™ ECL™ Prime is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.

Product Image-17-0455-01

Pharmalytes® IEF carrier ampholytes

Supplier: Cytiva
Description: Pharmalyte® carrier ampholytes, prepared by the co-polymerization of glycine, glycylglycine, amines and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.

MSDS Certificates

Product Image-RPN2125

Western blotting blocking reagent, Amersham™ ECL™

Catalog Number: (RPN2125)
Supplier: Cytiva
Description: Amersham ECL Blocking Agent is for blocking nitrocellulose and PVDF membranes to be used with Amersham ECL, Amersham ECL Prime and Amersham ECL Select Western Blotting Detection Reagents
UOM: 1 * 40 g
Product Image-PA63101

CyDye™ Mono-Reactive Maleimide Fluorescent Dyes, Cytiva

Supplier: Cytiva
Description: CyDye™ Mono-Reactive Maleimide Fluorescent Dyes are intensely fluorescent and highly water soluble, providing significant advantages over other existing fluorophores.

Product Image-RPN2105

Amersham™ ECL Direct™ Detection Reagents, Cytiva

Supplier: Cytiva
Description: Amersham ECL detection reagent uses enhanced luminol-based detection suitable for all routine confirmatory Western blotting experiments.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
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Dual use goods can only be delivered within the European Union.
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