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PowerPoint Presentation Comparative Proteomics Kit II: Western Blot Module Comparative Proteomics Kit II: Western Blot Module Instructors Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville CA Kirk Brown Lead...

1.0 0.9 0.8 0.7 0.6 0.5 rM A b (g /L ) 0.4 0.3 0.2 0.1 0 10 9 8 7 6 5 C el l d en si ty (c el ls /m L) 4 3 2 1 0 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Time (d) Feed Application note 29188433 AA GE Healthcare Production of recombinant monoclonal antib...

1.0 0.9 0.8 0.7 0.6 0.5 rM A b (g /L ) 0.4 0.3 0.2 0.1 0 10 9 8 7 6 5 C el l d en si ty (c el ls /m L) 4 3 2 1 0 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Time (d) Feed Application note 29188433 AA GE Healthcare Production of recombinant monoclonal antib...

1.0 0.9 0.8 0.7 0.6 0.5 rM A b (g /L ) 0.4 0.3 0.2 0.1 0 10 9 8 7 6 5 C el l d en si ty (c el ls /m L) 4 3 2 1 0 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Time (d) Feed Application note 29188433 AA GE Healthcare Production of recombinant monoclonal antib...

1.0 0.9 0.8 0.7 0.6 0.5 rM A b (g /L ) 0.4 0.3 0.2 0.1 0 10 9 8 7 6 5 C el l d en si ty (c el ls /m L) 4 3 2 1 0 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Time (d) Feed Application note 29188433 AA GE Healthcare Production of recombinant monoclonal antib...

Proteomics PROTEIN EXPRESSION ☐ Protein Expression Mammalian Routine Tightly Controlled Ultra-Pure ☐ Prokaryotic Cells Competent Cells Electroporation Induction Reagents PET Vectors for Gateway Cloning ☐ Transfection Insect Cells Mammalian Cells ☐...

1.0 0.9 0.8 0.7 0.6 0.5 rM A b (g /L ) 0.4 0.3 0.2 0.1 0 10 9 8 7 6 5 C el l d en si ty (c el ls /m L) 4 3 2 1 0 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Time (d) Feed Application note 29188433 AA GE Healthcare Production of recombinant monoclonal antib...

1.0 0.9 0.8 0.7 0.6 0.5 rM A b (g /L ) 0.4 0.3 0.2 0.1 0 10 9 8 7 6 5 C el l d en si ty (c el ls /m L) 4 3 2 1 0 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Time (d) Feed Application note 29188433 AA GE Healthcare Production of recombinant monoclonal antib...

Equipment ☐ Anaerobic Systems Chambers Indicators Jars ☐ Anesthesia Equipment ☐ Autoclaves/Sterilizers Portable Top-Loading ☐ Blenders ☐ Cell Counters ☐ Centrifuges Benchtop Centrifugal Concentrators Clinical Floor/Ultra Micro/Mini Refrigerated Ro...

PRODUCT CHECKLIST Equipment ☐ Anaerobic Systems Chambers Indicators Jars ☐ Anesthesia Equipment ☐ Autoclaves/Sterilizers Portable Top-Loading ☐ Blenders ☐ Cell Counters ☐ Centrifuges Benchtop Centrifugal Concentrators Clinical Floor/Ultra Micro/Mi...

1.0 0.9 0.8 0.7 0.6 0.5 rM A b (g /L ) 0.4 0.3 0.2 0.1 0 10 9 8 7 6 5 C el l d en si ty (c el ls /m L) 4 3 2 1 0 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Time (d) Feed Application note 29188433 AA GE Healthcare Production of recombinant monoclonal antib...

1.0 0.9 0.8 0.7 0.6 0.5 rM A b (g /L ) 0.4 0.3 0.2 0.1 0 10 9 8 7 6 5 C el l d en si ty (c el ls /m L) 4 3 2 1 0 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Time (d) Feed Application note 29188433 AA GE Healthcare Production of recombinant monoclonal antib...

1.0 0.9 0.8 0.7 0.6 0.5 rM A b (g /L ) 0.4 0.3 0.2 0.1 0 10 9 8 7 6 5 C el l d en si ty (c el ls /m L) 4 3 2 1 0 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Time (d) Feed Application note 29188433 AA GE Healthcare Production of recombinant monoclonal antib...

Présentation PowerPoint Advanced CHO Cell Expression System for Increased Transient Protein Production 0 20 40 60 80 100 0 2 4 6 8 10 12 0 24 48 72 96 120 V ia b ili ty ( % ) V ia b le C el l D en si ty ( x1 0 6 ce lls /m L) Time (h) Viable Cell D...

Présentation PowerPoint Advanced CHO Cell Expression System for Increased Transient Protein Production 0 20 40 60 80 100 0 2 4 6 8 10 12 0 24 48 72 96 120 V ia b ili ty ( % ) V ia b le C el l D en si ty ( x1 0 6 ce lls /m L) Time (h) Viable Cell D...

Présentation PowerPoint Advanced CHO Cell Expression System for Increased Transient Protein Production 0 20 40 60 80 100 0 2 4 6 8 10 12 0 24 48 72 96 120 V ia b ili ty ( % ) V ia b le C el l D en si ty ( x1 0 6 ce lls /m L) Time (h) Viable Cell D...