HIF-1α transcription factor assay Kit

Supplier: Cayman Chemical
CAYM10006910-96EA 654 EUR
CAYM10006910-96
HIF-1α transcription factor assay Kit
Assays Cellular Assays
HIF-1α Transcription Factor Assay Kit is a sensitive, non radioactive method for detecting HIF-1α from whole cell lysates.

  • 96-well plate format replaces EMSAs
  • Capture the transcription factor using a specific dsDNA sequence bound to the plate
  • Detect the dsDNA-bound transcription factor with specific antibodies in an ELISA format

The HIF (hypoxia-inducible factor) transcription factor is a member of the basic-helix-loop-helix (bHLH) family of transcription factors and plays an important role in maintaining cellular oxygen homeostasis. HIF-1α has emerged as an important drug target in breast and prostate cancer, cardiovascular disease, and ischemia.

HIF-1αTranscription Factor Assay is a sensitive ELISA-based method for detecting HIF-1α DNA binding activity in nuclear extracts and whole cell lysates.

Delivery information: Kit contains: Assay buffer, Reagent A, positive control, binding buffer, antibody, concentrate, polysorbate 20, competitor dsDNA, HRP conjugate, plate and cover, developing and stop solution.
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