3,3',5,5'-Tetramethylbenzidine (TMB) 98, Sigma-Aldrich®

Supplier: Merck

Synonyms: TMB, 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine

MQ300

87748-1G 87748-25G 87748-5G
SIAL87748-1GEA 109 EUR
SIAL87748-1G SIAL87748-25G SIAL87748-5G
3,3',5,5'-Tetramethylbenzidine (TMB) 98, Sigma-Aldrich®
3,3',5,5'-Tetramethylbenzidine (TMB)

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures.


The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Formula: C₁₆H₂₀N₂
MW: 240.35 g/mol
Boiling Pt: 402
Melting Pt: 166…170 °C
Storage Temperature: Ambient
MDL Number: MFCD00007748
CAS Number: 54827-17-7
EINECS: 259-364-6

Order Now


Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR