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You Searched For: Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.


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Product Image-PIER89880

Pierce™ Chemiluminescent Nucleic Acid Detection Module Kit

Supplier: Thermo Fisher Scientific
Description: The Pierce™ Chemiluminescent nucleic acid detection module is a complete system for the detection of biotin-labeled nucleic acids for various blotting applications, including Northern/Southern blots, ribonuclease protection assays (RPAs), and electrophoretic mobility shift assays (EMSAs) or gel-shift assays.

Product Image-PIER34065

DAB substrate kit, metal enhanced, Pierce™

Catalog Number: (PIER34065)
Supplier: Thermo Fisher Scientific
Description: The Pierce™ Metal enhanced DAB substrate kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate that yields intense colour for immunohistochemical staining.
UOM: 1 * 250 mL
Product Image-PIER15168

West HisProbe kit, SuperSignal™

Catalog Number: (PIER15168)
Supplier: Thermo Fisher Scientific
Description: SuperSignal™ West HisProbe Kit contains HisProbe-HRP conjugate and reagents required for detection of polyhistidine-tagged protein by Western blotting. HisProbe-HRP Conjugate is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins.
UOM: 1 * 1 KIT
Product Image-786-123T

Sample clean up, PAGE-Perfect™

Supplier: G-Biosciences
Description: Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis. The presence of contaminants, or interfering agents, such as salts, acids, bases and detergents, result in band distortion and poor protein resolution. As a result, SDS-PAGE gels are hard to analyse and lack reproducibility. PAGE-Perfect™ is a simple, two-step method for concentrating, cleaning and preparing protein solutions for running publication quality gels.

Image Unavailable-J60561.AK

SSC (20X), RNAse free

Supplier: Thermo Fisher Scientific
Description: SSC solution is commonly used in nucleic acid hybridization techniques at concentrations ranging from 0,1X to 20X depending on the application. It is used to facilitate transfer of nucleic acids to membranes in Southern and Northern blotting, dot blotting, and colony and plaque lifts.

MSDS Certificates

Product Image-ROCKFEMTOMAX-020

Chemiluminescent super sensitive HRP substrate, FemtoMax™

Supplier: Rockland Immunochemicals
Description: FemtoMax™ is an extremely sensitive, non radioactive, and enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP). It allows for the detection of femtogram (10 to 15) amounts of antigen using photographic film or other imaging methods including highly sensitive CCD cameras.

Product Image-PIER35065

Fast western blotting kit, SuperSignal™ west Pico, Pierce™

Supplier: Thermo Fisher Scientific
Description: The Fast Western Blot Kit, SuperSignal™ West Pico Substrate contains optimised reagents and protocol for picogram-level detection and and Western blot development less than 1 hour after gel transfer.

Product Image-ROCK88-8884-31

Western blot kits, TrueBlot®

Supplier: Rockland Immunochemicals
Description: TrueBlot® Western blot kits contain the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot® second step immunoblotting reagents in conjunction with the user's own primary IP antibody and primary (mouse, goat or rabbit IgG) Western blotting antibody.

MSDS

Image Unavailable-J63209.AK

Saponin permeating solution, 0,5% w/v solution in PBS (10X)

Catalog Number: (J63209.AK)
Supplier: Thermo Fisher Scientific
Description: Saponin permeating solution, is used as a cell permeabilising agent.
UOM: 1 * 250 mL

MSDS Certificates


Product Image-786-124

Sample clean up, Perfect-FOCUS™

Supplier: G-Biosciences
Description: Perfect-FOCUS™ is based on quantitative precipitation and concentration of protein solutions using UPPA™ (Universal Protein Precipitation Agent) and has been specifically developed for preparing lower conductivity protein samples for iso-electric focusing gels. Perfect-FOCUS™ is designed to clean and remove interfering agents from protein samples and concentrate protein solutions that give poor protein spot resolution during 2D electrophoresis.

Product Image-PIER35081

SuperSignal™, Pierce™ Fast Western Blotting Kit, West Femto

Supplier: Thermo Fisher Scientific
Description: The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes.

Image Unavailable-J61462.AK

Luminol and oxidising solutions

Supplier: Thermo Fisher Scientific
Description: For chemiluminecent HRP-based detection of western blotting systems. It is soluble in most polar organic solvents but is insoluble in water.

MSDS Certificates

Product Image-PIER35071

Fast western blot kit, SuperSignal™ West Dura, Pierce™

Supplier: Thermo Fisher Scientific
Description: The Fast Western Blot Kits, SuperSignal™ West Dura provide reagents and protocol for reliable and rapid, 1 hour chemiluminescent Western blotting at femtogram levels of sensitivity. These complete Fast Western Blot Kits requires minimal hands on time and yield results comparable to classical Western blotting with SuperSignal™ West Dura Chemiluminescent Substrate. This substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse or rabbit primary antibody.

Product Image-ROCKKCA001

Chemiluminescent western blot kits, FemtoMax™

Supplier: Rockland Immunochemicals
Description: These Chemiluminescent Western Blot Kits allow for the detection of primary polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane-bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialised formulation of luminol, an extremely sensitive, non radioactive substrate that emits light and allows visualisation using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of the kit, primary and secondary antibodies can be used at greater dilutions. Primary antibodies currently diluted 1:1000 using an ECL™ substrate is the equivalent of a 1:5000 dilution using the FemtoMax™ substrate.

Product Image-TB110

Kingdom blot

Catalog Number: (TB110)
Supplier: G-Biosciences
Description: Kingdom blot is prepared using total protein extracted from a variety of species, where whole species is not available the whole liver lysate is used. These blots can be used to identify similar proteins in other species, including human, chicken, frog, worm, Drosophila, yeast, Aspergillus, <i>E. coli</i>, Rhizobium and green algae.
UOM: 1 * 1 items
Image Unavailable-A3417.5000

CheLuminate-HRP PicoDetect Chemiluminescent Detection Kit

Catalog Number: (A3417.5000)
Supplier: PanReac AppliChem
Description: The CheLuminate-HRP PicoDetect is a complete kit with ready to use reagents for chemiluminescent detection of immobilised proteins (Western blot) or immobilised nucleic acids (Southern or Northern blot), conjugated with horseradish peroxidase (HRP) directly or indirectly.
UOM: 1 * 1 KIT
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
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The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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