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You Searched For: Protein Extraction Reagents

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.


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Product Image-786-791

Immobilised Pepsin

Catalog Number: (786-791)
Supplier: G-Biosciences
Description: A proteolytic enzyme immobilised on 4% agarose that is routinely used for the generation of F(ab’)₂ fragments from Immunoglobulin G (IgG). The pepsin has the ability to cleave the heavy chains near the hinge region. The immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.
UOM: 1 * 1 items
Product Image-786-790

Immobilised Papain

Catalog Number: (786-790)
Supplier: G-Biosciences
Description: A cysteine protease enzyme (EC 3.4.22.2) immobilised on 4% agarose, cleaves Immunoglobulin G (IgG) antibody molecules in the hinge region, generating three ~50 kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonisation, and lysis.
UOM: 1 * 1 items
Product Image-PIER88216

Micrococcal nuclease solution (≥100 U/µl)

Catalog Number: (PIER88216)
Supplier: Thermo Fisher Scientific
Description: This Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.
UOM: 1 * 1 items
Product Image-PIER20308

SDS precipitation kit, SDS-Out™

Catalog Number: (PIER20308)
Supplier: Thermo Fisher Scientific
Description: SDS-Out™ SDS Precipitation Kit provides convenient removal of SDS from small-volume (0,1 to 10 ml) protein samples.
UOM: 1 * 1 KIT
Product Image-PIER28313

Detergent solutions, Surfact-Amps™

Supplier: Thermo Fisher Scientific
Description: Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques. These detergents are useful as a component of cell lysis buffers or other solutions intended to extract and solubilise proteins, improve chromatography, prepare yeast spheroplasts, and reduce background in ELISA or Western blot methods.

Product Image-PIER90305

Trypsin protease, MS grade

Supplier: Thermo Fisher Scientific
Description: Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.

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Product Image-786-680

Washing solution, Protein-OUT™

Catalog Number: (786-680)
Supplier: G-Biosciences
Description: Protein-OUT™ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUT™ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUT™ removes inadvertent contamination with dust and keratin.
UOM: 1 * 50 mL
Product Image-REST25850

QuEChERS extraction salts, Q-sep®

Supplier: Restek
Description: QuEChERS methods are fast, easy, and cost-effective, and Restek Q-sep® products make QuEChERS procedures even simpler.

Image Unavailable-VARIPL6204-6103

Uniform latex particles for immobilisation of biomolecules, PL-Latex

Supplier: VARIAN
Description: Uniform latex particles are used in a wide range of applications that exploit their physico-chemical properties in sophisticated immunoassay formats, both in medicine and veterinary science research.

Image Unavailable-CEMATMS001-4X4L

TamiSolve® NxG-PS Solvents for Peptide Synthesis

Catalog Number: (CEMATMS001-4X4L)
Supplier: CEM
Description: Modern peptide synthesis agents can dramatically reduce waste generation, but challenges remain regarding the health and environmental impacts of commonly used solvents.
UOM: 1 * 4 L

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
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