You Searched For: 4-Methoxyphenyl-beta-D-galactopyranoside

Supplier: Biotium

Description: Chromogenic and fluorogenic beta-galactosidase substrates.

Catalog Number: (ROCH10651745001)

Supplier: ROCHE

Description: X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)

UOM: 1 * 250 mg

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Catalog Number: (EDVO614)

Supplier: EDVOTEK

Description: X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)

UOM: 1 * 250 mg

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Supplier: Ambion

Description: X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)

Supplier: Thermo Fisher Scientific

Description: X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) ≥98%

MSDS

Catalog Number: (OZBIGX10003)

Supplier: OZ BIOSCIENCES

Description: X-Gal staining kit allows to visualise ß-gal expression through hydrolysis of the X-Gal (5-bromo-4-chloro-3-indoyl-beta-D-Galactopyranoside) substrate yielding blue precipitates.

UOM: 1 * 1 KIT

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Catalog Number: (MOLEM30473975)

Supplier: Molekula

Description: X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)

UOM: 1 * 100 mg

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Catalog Number: (MOLE11322533-5G)

Supplier: Molekula

Description: X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)

UOM: 1 * 5 g

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Catalog Number: (RC-113)

Supplier: G-Biosciences

Description: X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)

UOM: 1 * 100 mg

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Supplier: Roth Carl

Description: X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)

Catalog Number: (ROCH10745740001)

Supplier: ROCHE

Description: X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)

UOM: 1 * 1 g

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Supplier: Merck

Description: Suitable as substrate for β-galactosidase.

Supplier: MP Biomedicals

Description: 5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside, commonly known as X-Gal, is a histochemical substrate for b-galactosidase. X-Gal is cleaved by b-galactosidase to yield an insoluble blue precipitate.

Supplier: G-Biosciences

Description: X-gal (5-bromo-4-chloro-3-indolyl-β-D- galactopyranoside) is used to indicate whether bacteria express the ß-galactosidase enzyme, which is encoded by the lacZ gene. X-gal is cleaved by ß-galactosidase yielding galactose and 5-bromo-4-chloro-3-hydroxyindole, which is oxidised into 5,5'-dibromo-4,4'-dichloro-indigo, an insoluble blue product. Thus, if X-gal and an inducer of ß-galactosidase (usually IPTG) is contained within an agar medium on a culture plate, colonies which have a functional lacZ gene can easily be distinguished by their blue colour.

Catalog Number: (OZBIXG31000)

Supplier: OZ BIOSCIENCES

Description: X-Gal substrate is metabolised by the b-galactosidase enzyme. The quality and purity of the substrate is essential to obtain high-quality and reliable results.

UOM: 1 * 1 g

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Supplier: MP Biomedicals

Description: Storage: Store at -20 °C. Store Desiccated. Store Under Nitrogen. Protect from light.
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, commonly known as X−Gal, is a histochemical substrate for β−galactosidase.
5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. It is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. X−Gal is cleaved by β−galactosidase to yield an insoluble blue precipitate. X−Gal is particularly useful in molecular biology applications to detect the activity of β−galactosidase which is frequently used as a reporter gene. In cloning, X−Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α-complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase.