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Urea ≥98%, white powder for molecular biology

Supplier: MP Biomedicals

Description: Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Sodium tetrachloropalladate(II) hydrate (≥30% Pd) ≥99.999% (metals basis), Premion®

Supplier: Thermo Fisher Scientific

Description: Sodium tetrachloropalladate(II) hydrate (≥30% Pd) ≥99.999% (metals basis), Premion®

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Anti-CHAMP1 Rabbit Polyclonal Antibody

Catalog Number: (PRSI27-948)

Supplier: ProSci Inc.

Description: The CD233 gene is located on chromosome 17q21-q22 and is part of the anion exchanger (AE) family. CD233 is expressed in the erythrocyte plasma membrane where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. CD233 associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of CD233. CD233 is predominantly dimeric but forms tetramers in the presence of ankyrin. Many CD233 mutations are known in man and these mutations can lead to two types of disease; destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other CD233 mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system. Southeast Asian ovalocytosis (SAO, Melanesian ovalocytosis) results from the heterozygous presence of a deletion in the CD233 protein and is common in areas where Plasmodium falciparum malaria is endemic. One CD233 null human is known also with very severe anemia and nephrocalcinosis [PROW].

UOM: 1 * 50 µG

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Pinacyanol chloride, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Description: Pinacyanol chloride, (1,1'-Diethyl-2,2'-carbocyanine chloride; 2,2'-Trimethinequinocyanine chloride; Quinaldine blue).

Silver chloride ≥99%, TECHNICAL

Catalog Number: (21553.137)

Supplier: VWR Chemicals

Description: Silver chloride ≥99%, TECHNICAL

UOM: 1 * 25 g

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Gold chloride solution 200 mg/dl in deionised water

Catalog Number: (HT1004-100ML)

Supplier: SIGMA ALDRICH MICROSCOPY

Description: Used in procedure HT100 for the toning of sections stained with the silver methanamine procedure.

UOM: 1 * 100 mL

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Zinc chloride ≥97%, TECHNICAL

Supplier: VWR Chemicals

Description: Zinc chloride ≥97%, TECHNICAL

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Lithium chloride ≥99% for biotechnology

Supplier: VWR Chemicals

Description: Lithium chloride ≥99% for biotechnology

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2,3,5-Triphenyltetrazolium chloride (TTC) ≥98% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Description: 2,3,5-Triphenyltetrazolium chloride (TTC) ≥98% (by HPLC), Sigma-Aldrich®

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Cesium chloride ≥98% Grade II, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Description: It is used for the preparation of electrically conducting glasses. Used to make solutions for the separation of RNA from DNA by density gradient centrifugation.