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Osteoarthritic human chondrocytes P0 (50-70y), CELLvo™

Supplier: STEMBIOSYS
Description: CELLvo™ Ostoeoarthritic Human Chondrocytes (50-70y) are primary, uncultured cells isolated from recently-diseased cadaver cartilage from donors between 50 and 70 years old. Osteoarthritis status is determined by macroscopic inspection using a modified Outerbridge (1960) scale. Cells are isolated by enzymatic digestion prior to cryopreservation.

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Uridine ≥99%, white powder

Supplier: MP Biomedicals
Description: Uridine is one of the four basic components of ribonucleic acid (RNA); the other three are adenosine, guanosine, and cytidine. Upon digestion of foods containing RNA, uridine is released from RNA and is absorbed intact in the gut. Uridine plays a role in the glycolysis pathway of galactose.
Uridine is used in treatment of bipolar disorder associated with mood swings.Uridine is used in metabolic labeling and analysis of rRNA processing.

Product Image-SYGEGA1100-4X50

Gibson Assembly® HiFi HC Cloning Kits and Master Mixes

Supplier: SGI - DNA Synthetic Genomics
Description: Gibson Assembly® HiFi HC 1-Step Kits and Master Mixes allow restriction digest-free, cloning of one or more DNA fragments into virtually any location of any plasmid vector in a single round of cloning.

Product Image-EDVO105-C

Kits: mapping of restriction sites on plasmid DNA, Ready-to-Load™

Supplier: EDVOTEK
Description: DNA mapping is a common procedure used to determine the location of genes. In this experiment, DNA markers and pre-digested plasmid DNA fragments are mapped using agarose gel electrophoresis.

Product Image-F-537L

Phusion® Green hot start II High-fidelity DNA polymerase

Supplier: FINNZYMES REAGENTS
Description: Phusion® Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion® Hot Start II DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® Hot Start II DNA polymerase and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Image Unavailable-PRSI92-415

Mouse recombinant carboxypeptidase A4

Catalog Number: (PRSI92-415)
Supplier: ProSci Inc.
Description: Carboxypeptidase A4 (CPA4) is a member of the peptidase M14 family. CPA4 is metalloprotease that could be involved in the histone hyperacetylation pathway. CPA4 binds one zinc ion per subunit and could catalyse to release of a C-terminal amino acid, with preference for -Phe, -Leu, -Ile, -Met, -Tyr and -Val.They have distinct expression patterns and different specificities for example, preferentially cleaving aromatic (carboxypeptidase As) or basic (carboxypeptidase Bs) residues. Several, such as carboxypeptidase Xs, have lost their catalytic activity. Carboxypeptidases play important roles in digestion of food, processing of bioactive peptides and blood coagulation.
UOM: 1 * 50 µG
Product Image-705-0191

Total organic carbon (TOC) reagent sets, Test 'N Tube™

Supplier: Hach
Description: Used for determination of Total Organic Carbon (TOC) either by low range, mid range or high range test 'N Tube' method respectively. The set includes digestion vials and reagents for 25 to 50 tests.

Product Image-786-680

Washing solution, Protein-OUT™

Catalog Number: (786-680)
Supplier: G-Biosciences
Description: Protein-OUT™ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUT™ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUT™ removes inadvertent contamination with dust and keratin.
UOM: 1 * 50 mL
Image Unavailable-PRSI33-194

Anti-PTPRC Mouse Monoclonal Antibody [clone: UCHL-1]

Catalog Number: (PRSI33-194)
Supplier: ProSci Inc.
Description: This antibody recognises a 180-185 kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
UOM: 1 * 100 µG

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Anti-Macrophage Specific Antigen Mouse Monoclonal Antibody [clone: LN-5]

Catalog Number: (BNUM0059-50)
Supplier: Biotium
Description: This MAb stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffer s cells of the liver and Langerhan s cells of the skin. Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and pro-monocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becoming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). LN-5 selectively stains human sebaceous glands in formalin-fixed, paraffin-embedded skin samples. Undifferentiated sebocyte progenitors are negative, and only sebocytes from the onset of their differentiation reveal positive cytoplasmic staining. Since there are very few selective and easy-to-use markers of sebaceous glands, LN-5 antibody can offer a simple and relatively specific way to detect human sebocytes from the onset of their.
UOM: 1 * 50 µl
Image Unavailable-PRSI91-568

Human recombinant Pancreatic Lipase-Related 1 (from cells)

Catalog Number: (PRSI91-568)
Supplier: ProSci Inc.
Description: Pancreatic Lipase-Related Protein 1 (PNLIPRP1) belongs to the Lipase family within the AB hydrolase superfamily. PNLIPRP1 is a secreted protein and contains one PLAT domain. PNLIPRP1 is involved in lipid metabolic process, acting as a negative regulator of pancreatic lipase activity by competing with pancreatic lipase for colipase occupancy. PNLIPRP1 may play a role in inhibiting dietary triglyceride digestion, but it lacks detectable lipase activity towards triglycerides, diglycerides, phosphatidylcholine, galactolipids or cholesterol esters.
UOM: 1 * 50 µG
Image Unavailable-PRSI91-821

Mouse recombinant cathepsin D

Catalog Number: (PRSI91-821)
Supplier: ProSci Inc.
Description: CTSD localizes to the lysosome and consists of a light chain and a heavy chain. CTSD is expressed in epithelial cells as well as in macrophages.CTSD is a lysosomal aspartyl protease that depends critically on protonation of its active site Asp residue and gets activated at pH 5 in endosome of hepatocytes. It has been suggested to facilitate cancer cell migration and invasion by digesting the basement membrane, extracellular matrix and xonnective tissue. In addition, CTSD has been used as a breast cancer tumor marker.
UOM: 1 * 50 µG
Product Image-BARNSP135930-33Q

SuperNuova+™ Multi-Position Magnetic Stirrers

Supplier: Thermo Scientific
Description: Carry out solubility studies, digestion, titration and enzymatic studies with Thermo Scientific™ Super-Nuova™ Multi-Position Digital Stirrers. Offering four individually-controlled stirring positions, ceramic tops and 4-function memory, these stirrers are designed for stronger coupling and stirring control with advanced safety features. Ideal for labs with multiple users.

Product Image-EDVO302

Experimentation kits, purification of the restriction enzyme Eco RI

Catalog Number: (EDVO302)
Supplier: EDVOTEK
Description: In this experiment, students purify the restriction enzyme, <i>Eco </i>RI. This procedure utilised an ion exchange chromatograph step for <i>Eco </i>RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by gel electrophoresis. Fractions that contain <i>Eco </i>RI are identified and pooled. The total and specific activities are calculated. Recommended for advanced courses.
UOM: 1 * 1 KIT
Product Image-APPC252908.1608

Kovac's indole reagent for microbiology

Catalog Number: (APPC252908.1608)
Supplier: PanReac AppliChem
Description: A reagent for the detection of indole-positive and indole-negative microorganisms. Some microorganisms can cleave tryptophan which is especially abundant in trypticalle digested peptone to give pyruvic acid, ammonia and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a colour reaction with 4-dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.
UOM: 1 * 100 mL

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Image Unavailable-PRSI91-777

Human recombinant CGREF1 (from cells)

Catalog Number: (PRSI91-777)
Supplier: ProSci Inc.
Description: Cell Growth Regulator with EF Hand Domain Protein 1 (CGREF1) is a secreted calcium ion binding protein. CGREF1 contains two EF-hand domains and both EF-hands are required for function. Human CGREF1 is synthesized as a 301 amino acid precursor that contains a 19 amino acid signal sequence, and a 282 amino acid mature chain. CGREF1 is probably digested extracellularly by an unknown serine protease generating extremely hydrophobic bioactive peptides. CGREF1 mediates cell-cell adhesion in a calcium-dependent manner. In addition, CGREF1 is able to inhibit growth in several cell lines.
UOM: 1 * 50 µG
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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