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Urea ≥98%, white powder for molecular biology

Supplier: MP Biomedicals
Description: Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Image Unavailable-43945.06

Sodium tetrachloropalladate(II) hydrate (≥30% Pd) ≥99.999% (metals basis), Premion®

Supplier: Thermo Fisher Scientific
Description: Sodium tetrachloropalladate(II) hydrate (≥30% Pd) ≥99.999% (metals basis), Premion®

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Product Image-PRSI27-948

Anti-CHAMP1 Rabbit Polyclonal Antibody

Catalog Number: (PRSI27-948)
Supplier: ProSci Inc.
Description: The CD233 gene is located on chromosome 17q21-q22 and is part of the anion exchanger (AE) family. CD233 is expressed in the erythrocyte plasma membrane where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. CD233 associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of CD233. CD233 is predominantly dimeric but forms tetramers in the presence of ankyrin. Many CD233 mutations are known in man and these mutations can lead to two types of disease; destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other CD233 mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system. Southeast Asian ovalocytosis (SAO, Melanesian ovalocytosis) results from the heterozygous presence of a deletion in the CD233 protein and is common in areas where Plasmodium falciparum malaria is endemic. One CD233 null human is known also with very severe anemia and nephrocalcinosis [PROW].
UOM: 1 * 50 µG
Image Unavailable-201715-5G

Pinacyanol chloride, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY
Description: Pinacyanol chloride, (1,1'-Diethyl-2,2'-carbocyanine chloride; 2,2'-Trimethinequinocyanine chloride; Quinaldine blue).

Product Image-25009.464

Lithium chloride ≥98%, purified

Supplier: VWR Chemicals
Description: Lithium chloride ≥98%, purified

MSDS Certificates

Image Unavailable-264806-5G

(2,5-Dimethoxyphenyl)acetyl chloride 99%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY
Description: (2,5-Dimethoxyphenyl)acetyl chloride 99%, Sigma-Aldrich®

Product Image-24706.238

Hydroxylammonium chloride, TECHNICAL

Catalog Number: (24706.238)
Supplier: VWR Chemicals
Description: Hydroxylammonium chloride, TECHNICAL
UOM: 1 * 250 g

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Product Image-87765.290

Aluminium chloride Reag. Ph. Eur. 1002702

Catalog Number: (87765.290)
Supplier: VWR Chemicals
Description: Starting materials: Aluminium chloride R / Glacial acetic acid R/ Methanol R.
UOM: 1 * 1 L

MSDS Certificates


Product Image-21467.152

Antimony(V) chloride

Catalog Number: (21467.152)
Supplier: VWR Chemicals
Description: Antimony(V) chloride
UOM: 1 * 50 mL

MSDS


Product Image-X190-5KG

Sodium chloride, high purity

Supplier: VWR Chemicals
Description: Sodium chloride, high purity

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Image Unavailable-I8377-5G

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride colorimetric assays, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY
Description: Iodonitrotetrazolium chloride has been used for the staining of cells. It has been used as a coupling agent in the enzymatic assay of D-arabinitol.

Image Unavailable-732-3315

Lithium chloride, peqGOLD OptiPure

Catalog Number: (732-3315)
Supplier: VWR Peqlab
Description: Lithium chloride, peqGOLD OptiPure
UOM: 1 * 1 items

MSDS


Product Image-28804.121

2,3,5-Triphenyltetrazolium chloride (TTC), TECHNICAL

Catalog Number: (28804.121)
Supplier: VWR Chemicals
Description: 2,3,5-Triphenyltetrazolium chloride (TTC), TECHNICAL
UOM: 1 * 10 g

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Product Image-21553.137

Silver chloride ≥99%, TECHNICAL

Catalog Number: (21553.137)
Supplier: VWR Chemicals
Description: Silver chloride ≥99%, TECHNICAL
UOM: 1 * 25 g

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Product Image-87096.180

Phenylhydrazinium chloride ≥99%, AnalaR NORMAPUR® analytical reagent

Catalog Number: (87096.180)
Supplier: VWR Chemicals
Description: Phenylhydrazinium chloride ≥99%, AnalaR NORMAPUR® analytical reagent
UOM: 1 * 100 g

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Image Unavailable-HT1004-100ML

Gold chloride solution 200 mg/dl in deionised water

Catalog Number: (HT1004-100ML)
Supplier: SIGMA ALDRICH MICROSCOPY
Description: Used in procedure HT100 for the toning of sections stained with the silver methanamine procedure.
UOM: 1 * 100 mL
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
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The original product is no longer available. The replacement shown is available.
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