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Product Image-763-0001

Experimentation kits, amplification of DNA by PCR

Catalog Number: (763-0001)
Supplier: EDVOTEK
Description: In this PCR experiment, students make billions of copies of a small amount of DNA in only 90 minutes. They just need to mix template DNA and primers with PCR beads that contain all of the other components required to carry out a PCR reaction. Students see the increasing amounts of DNA for themselves, taking samples every few cycles and analysing them on a DNA gel.
UOM: 1 * 1 KIT
Product Image-BE-301

Experimentation kits, bacterial conjugation

Catalog Number: (BE-301)
Supplier: G-Biosciences
Description: Bacterial conjugation is a naturally occurring process that allows the transfer of DNA from one bacterium to another, which allows the transfer of genetic traits, particularly drug resistance. The kit contains two bacteria with different drug resistance genes and all the tools for students to study bacterial conjugation. This kit teaches the difference between bacterial genomic DNA and the transferable plasmid DNA and the mechanisms of bacterial conjugation. Students will also learn important basic microbiological techniques, including bacterial growth in liquid broth and on solid agar plates, antibiotic selection of bacteria and important aseptic techniques.
UOM: 1 * 1 KIT
Product Image-EDVO956

Experimentation kit, bioremediation by oil eating bacteria

Catalog Number: (EDVO956)
Supplier: EDVOTEK
Description: Oil spills cause devastation to the environment, killing sea life, birds, and coastal plants. Spraying areas of contamination with oil-eating microbes accelerates the degradation of the oil. This process is known as bioremediation. In this open-ended experiment, students grow a mixture of oil-eating bacteria and observe their effectiveness at degrading a variety of oils.
UOM: 1 * 1 KIT

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Product Image-EDVO315

Kits: in search of the sickle cell gene by southern blot, Ready-to-Load™

Catalog Number: (EDVO315)
Supplier: EDVOTEK
Description: Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane the method allows the precise analysis and identification of DNA bands on a gel. Using this kit, students use Southern blotting to find a point mutation in the haemoglobin gene indicating Sickle Cell Anaemia.
UOM: 1 * 1 items

MSDS


Product Image-BE-201

Experimentation kits, bacterial culture and growth study

Catalog Number: (BE-201)
Supplier: G-Biosciences
Description: This kit teaches aseptic handling techniques and cultivation of bacteria in liquid culture media and on solid phase agar plates. This kit is designed to educate students about the various stages of the bacterial growth cycle, i.e. lag, log or exponential, stationary and decline or death phases. The kit also teaches the importance of growing bacteria on solid phase agar plates to isolate single colonies of bacteria. This lab activity involves preparation of culture medium and solid agar plates.
UOM: 1 * 1 KIT
Product Image-28821.131

L(-)-Tryptophan ≥99% for biochemistry

Catalog Number: (28821.131)
Supplier: VWR Chemicals
Description: L(-)-Tryptophan ≥99% for biochemistry
UOM: 1 * 25 g

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Image Unavailable-APOSBIM111-5G

2-Iodosobenzoic acid

Supplier: Apollo Scientific
Description: Cleaves at carboxyl side of tryptophanRef: Mahoney,W.C.&Hermodson,M.A(1979)Biochemistry 18, 3810-3814

Product Image-EDVO255

Purification and size determination of green and blue fluorescent proteins

Catalog Number: (EDVO255)
Supplier: EDVOTEK
Description: When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP are used as an assay during their purification from an <i>E. coli</i> extract. The column fractions containing GFP or BFP are identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.
UOM: 1 * 1 KIT
Product Image-BE-203

Experimentation kits, antibiotic sensitivity and bacteria screening

Catalog Number: (BE-203)
Supplier: G-Biosciences
Description: Antibiotics play a crucial role in the manipulation, screening and killing of bacteria in a range of biotechnology processes. This kit specifically teaches the basic principles of antibiotics, bacterial resistance and susceptibility. Students learn and understand the use of antibiotic resistance in screening for infectious diseases. Utilising a bacterial strain, students learn the effects of different antibiotics and visualise bacterial sensitivity and resistance to the supplied antibiotics. This method involves the use of filter paper discs impregnated with a specified concentration of antibiotics on the surface of an agar plate containing microbial cells. This kit will enable students to analyse the inhibitory effects of different antibiotics on selected bacterial cells and then determine which antibiotic is the most suitable to treat a bacterial infection.
UOM: 1 * 1 KIT
Product Image-BE-415

Experimentation kits, ion exchange chromatography

Catalog Number: (BE-415)
Supplier: G-Biosciences
Description: Ion exchange chromatography is used to separate charged molecules from complex biological samples. The charged molecules bind to a solid support carrying an opposite charge to the molecule. Proteins contain regions of charged groups on the surface which interact with the ion exchange groups immobilised on the solid support (resin column). Immobilised proteins are eluted by changing either pH or the salt gradient or a combination of both. This lab activity involves preparation of a crude protein extract and running ion exchange chromatography for isolation of proteins.
UOM: 1 * 1 KIT
Product Image-EDVO117-C

Kits: detection of mad cow disease, Ready-to-Load™

Supplier: EDVOTEK
Description: Bovine spongiform encephalophathy (BSE), also known as Mad Cow Disease, is a neurodegenerative, fatal condition in cattle. Consuming BSE-infected beef is believed to be the cause of a similar condition in humans, Creutzfeldt-Jakob disease. In this experiment, students examine simulated PCR products from several feed mills, to determine any possible violations of the ban on including animal parts in cattle feed.

Product Image-PRSI50-207

Anti-MAPK1 Rabbit Polyclonal Antibody

Catalog Number: (PRSI50-207)
Supplier: ProSci Inc.
Description: Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002; Ahn, 1993; Tanoue and Nishida, 2003; Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).
UOM: 1 * 100 µl
Product Image-442714K

Polyethylene glycol 6,000 for biochemistry

Catalog Number: (442714K)
Supplier: VWR Chemicals
Description: Polyethylene glycol 6,000 for biochemistry
UOM: 1 * 500 g

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Product Image-EDVO908

Experimentation kits, introduction to plant cell culture

Catalog Number: (EDVO908)
Supplier: EDVOTEK
Description: Genetic modification of plants is a controversial area of biotechnology. All such experiments in plants begin with establishing plant cells in culture. This involves de-differentiating plant cells to form plant 'stem cells'. In this experiment, students establish cell cultures of African violets from leaves. They then use plant growth regulators to encourage root growth from the cultured cells, and produce a mature plant.
UOM: 1 * 1 KIT
Product Image-EDVO222

Transformation with blue and green fluorescent proteins

Catalog Number: (EDVO222)
Supplier: EDVOTEK
Description: The Green Fluorescent Protein (GFP) from the jellyfish <i>Aequorea victoria</i> is used extensively in all areas of science. Many organisms have been transformed with the GFP gene, the gene responsible for bioluminescence in jellyfish. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment, students transform bacteria either by GFP, BFP or both.
UOM: 1 * 1 items

MSDS


Product Image-28719.290

D-(+)-Trehalose dihydrate ≥98% for biochemistry

Catalog Number: (28719.290)
Supplier: VWR Chemicals
Description: D-(+)-Trehalose dihydrate ≥98% for biochemistry
UOM: 1 * 1 kg

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
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