Catalog Number:
(ICNA0855730)
Clearance
Supplier:
MP Biomedicals
Description:
The complement system provides innate defense against microbial infection and is a "complement" to antibody mediated immunity. The complement system consists of thirty five interacting plasma and membrane associated proteins which contribute to host-defense and initiate and amplify inflammation, even in the preimmune state where specific antibodies and lymphocytes are not available. In addition to the complement components themselves, this system also contains several soluble factors that prevent spontaneous complement activation from occurring in solution, as well as, several regulatory membrane associated proteins that protect host cells from accidental complement attack. Cleavage of the C3 component by either the classical pathway or the alternative pathway releases C3a and C3b. C3a appears to be important in many inflammatory responses while the C3b fragment covalently binds to the cell or bacterial surface and plays a role in opsonisation. Binding of C3b to the C4b component of the C3 convertase, results in C5 convertase (C4b3b2a) formation
The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized to an in-house standard by equivalence-point precipitation. Each IgG fraction is tested for purity and specificity at a minimum of 40 mg/mL using immunoelectrophoresis. The product is mostly goat IgG; no trace of albumin is detected.
This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. The product has been successfully used in Immunoblotting. It detects C3 from human, equine, hamster, Guinea pig, murine, rabbit, rat, pig, and non-human primate samples. It does not cross react wtih bovine, canine, chicken, feline and sheep samples. It has been successfully used in immunocytochemistry, Western blot, Immunoelectrophoresis, and flow cytometry applications. It detects C3 from human, equine, hamster, Guinea pig, murine, rabbit, rat, pig, and non-human primate samples. It does not cross react wtih bovine, canine, chicken, feline and sheep samples. It has been successfully used in immunocytochemistry, Western blot, Immunoelectrophoresis, and flow cytometry applications.
UOM:
1 * 2 mL
