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Cell viability assay kits

Supplier: Biotium
Description: MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.

MSDS Certificates

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TOTAL-NUCLEAR-ID® green/red nucleolar/nuclear detection kit

Catalog Number: (ENZOENZ51006500)
Supplier: ENZO LIFE SCIENCES
Description: The kit contains two proprietary dyes suitable for visualizing nucleoli and nuclei simultaneously in living cells.
UOM: 1 * 1 KIT
Product Image-MLDVR8174

Neurotransmitter transporter uptake assay kits

Supplier: Molecular Devices
Description: The Neurotransmitter Transporter Uptake Assay Kit provides a fast, simple and reliable fluorescence-based assay for the detection of dopamine, norepinephrine and serotonin transporter (SERT, NET and DAT, respectively) activity in expressing cells.

Product Image-MLDVR8348

Cell viability assay kits, EarlyTox™ Caspase-3/7 and Caspase-3/7-D NucView™ 488 Assay Kits

Supplier: Molecular Devices
Description: EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.

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Ubiquitin activating kit

Catalog Number: (ENZOBMLUW0400A0001)
Supplier: ENZO LIFE SCIENCES
Description: Ubiquitin Activating Kit for activation of ubiquitin for use in ubiquitinylation experiments. The kit provides the means of generating thioester linked, activated ubiquitin-E1 conjugates, utilising the first step in the ubiquitin cascade, for investigation of ubiquitin activation, subsequent ubiquitin transfer to/interaction with E2 conjugating enzymes, and their use in the ubiquitinylation of E3 ligases and target substrate proteins. Ubiquitin antibody provided allows high sensitivity detection of ubiquitin and ubiquitin-conjugates.
UOM: 1 * 20 Tests
Product Image-MLDVR8211

Cardiotoxicity kit, EarlyTox™

Supplier: Molecular Devices
Description: The EarlyTox™ Cardiotoxicity Kit provides a fast, simple and reliable fluorescence based method for identifying cardiotoxic compounds in a biorelevant assay using stem-cell derived cardiomyocytes.

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CF® Dye Dextran 70000 MW, anionic and fixable

Supplier: Biotium
Description: CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 70,000 MW, and contains a formaldehyde-fixable free-amine group.

Product Image-80135.

CF® Dye Dextran 250000 MW, anionic and fixable

Supplier: Biotium
Description: CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 250,000 MW, and contains a formaldehyde-fixable free-amine group.

Product Image-80110.

CF® Dye Dextran 10000 MW, anionic and fixable

Supplier: Biotium
Description: CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 10000 MW, and contains a formaldehyde-fixable free-amine group.

Product Image-ENZOENZ51018K100

Membrane potential detection kit, MITO-ID®

Supplier: ENZO LIFE SCIENCES
Description: MITO-ID® membrane potential detection kit monitors energetic status using a simple mix-and-read, no-wash protocol. It measures mitochondrial membrane potential with a cationic dye that fluoresces either green or orange depending upon membrane potential status.

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Mitochondria/cytosol fractionation kit

Catalog Number: (ENZOALX850276KI01)
Supplier: ENZO LIFE SCIENCES
Description: For the effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of various mammalian cells including both apoptotic an non-apoptotic cells. The enriched mitochondrial and cytosolic fractions can be used to study apoptosis and signal trasduction pathways by detecting translocations of factors of interest between the two fractions by ELISA, Western blot or other assays.
UOM: 1 * 1 KIT
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Fluorescent nerve terminal probes and stains

Supplier: Biotium
Description: Nerve terminal probes are a series of fluorescent cationic styryl dyes developed to follow synaptic activity. These dyes have a lipophilic tail at one end and a hydrophilic cationically charged head group at the other end. The dyes vary in the number of carbons in the lipophilic tail (m) and the number of double bonds linking the two aromatic rings in the dye (n). AM dyes are similar to FM dyes, but contain an additional formaldehyde-fixable amino group in the cationic head.

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Apoptosis and necrosis quantitation kits

Supplier: Biotium
Description: Apoptosis and necrosis are two processes by which cells die. Apoptosis is an active, regulated disassembly of the cell from within. During apoptosis, phosphatidylserine is translocated from the inner to the outer surface of the cell, allowing the dying cell to be engulfed by phagocytic cells. Annexin V is a 35 kD Ca2+-dependent phospholipid binding protein with a high affinity for phosphatidylserine.

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ADAM17 fluorometric drug discovery kit

Catalog Number: (ENZOBMLAK3100001)
Supplier: ENZO LIFE SCIENCES
Description: Designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide. The ADAM17 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide: Mca-PLAQAV-Dpa-RSSSR-NH2. Mca fluorescence is quenched by the Dpa group until cleavage by proteases separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of ADAM17, a potential therapeutic target. The compound GM6001 is also included as a prototypic control inhibitor.
UOM: 1 * 1 KIT
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CFDA SE Cell proliferation assay kit

Catalog Number: (30050.)
Supplier: Biotium
Description: Cell proliferation dyes diffuse passively into cells and covalently label intracellular proteins, resulting in long term cell labelling. They are non-fluorescent until they are hydrolysed by intracellular esterase's. The dyes then react with intracellular amines forming fluorescent conjugates that are retained in the cell. Immediately after staining, a single, bright fluorescent population will be detected by flow cytometry. With each cell division, daughter cells inherit roughly half of the fluorescent label, allowing the number of cell divisions that occur after labelling to be detected by the appearance of successively dimmer fluorescent peaks on a flow cytometry histogram compared to cells analysed immediately after staining. Staining is formaldehyde fixable.
UOM: 1 * 1 KIT
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Live bacterial gram stain kit

Supplier: Biotium
Description: Wheat germ agglutinin binds specifically to N-acetylglucosamine in the peptidoglycan layer of gram-positive bacteria. The Live Bacterial Gram Stain Kit contains wheat germ agglutinin (WGA) conjugated to CF™594 to stain the surface of gram-positive cells with red fluorescence. The kit also includes DAPI to stain the DNA of all bacteria with blue fluorescence. Gram-positive bacteria stained using this kit will show a fluorescent blue interior and fluorescent red cell surface, while gram-negative bacterial will be stained with blue fluorescence only.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
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The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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