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FLUOR DE LYS® HDAC6 fluorometric drug discovery kit

Catalog Number: (ENZOBMLAK5160001)
Supplier: ENZO LIFE SCIENCES
Description: A Fluor-de-Lys® fluorescent assay system. The HDAC6 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC6 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions. The Fluor de Lys® HDAC6 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC6. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer produces a fluorophore.
UOM: 1 * 1 KIT
Product Image-MLDVR8211

Cardiotoxicity kit, EarlyTox™

Supplier: Molecular Devices
Description: The EarlyTox™ Cardiotoxicity Kit provides a fast, simple and reliable fluorescence based method for identifying cardiotoxic compounds in a biorelevant assay using stem-cell derived cardiomyocytes.

Image Unavailable-30050.

CFDA SE Cell proliferation assay kit

Catalog Number: (30050.)
Supplier: Biotium
Description: Cell proliferation dyes diffuse passively into cells and covalently label intracellular proteins, resulting in long term cell labelling. They are non-fluorescent until they are hydrolysed by intracellular esterase's. The dyes then react with intracellular amines forming fluorescent conjugates that are retained in the cell. Immediately after staining, a single, bright fluorescent population will be detected by flow cytometry. With each cell division, daughter cells inherit roughly half of the fluorescent label, allowing the number of cell divisions that occur after labelling to be detected by the appearance of successively dimmer fluorescent peaks on a flow cytometry histogram compared to cells analysed immediately after staining. Staining is formaldehyde fixable.
UOM: 1 * 1 KIT
Product Image-ENZOBMLUW09700001

Auto-Ubiquitinylation Kit

Catalog Number: (ENZOBMLUW09700001)
Supplier: ENZO LIFE SCIENCES
Description: This E3 ligase auto-ubiquitinylation kit enables proteins to be tested for ubiquitin E3 ligase activity through assessment of their ability to under auto-ubiquitinylation. Utilizing the first three steps in the ubiquitin cascade the kit facilitates ubiquitinylation of known or putative E3 ligase enzymes followed by Western blot analysis using the highly sensitive reagents provided or using antibodies to the specific protein of interest (user supplied). A high integrity ubiquitin E3 ligase enzyme is also provided for use as a positive control.
UOM: 1 * 10 Tests
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Live bacterial gram stain kit

Supplier: Biotium
Description: Wheat germ agglutinin binds specifically to N-acetylglucosamine in the peptidoglycan layer of gram-positive bacteria. The Live Bacterial Gram Stain Kit contains wheat germ agglutinin (WGA) conjugated to CF™594 to stain the surface of gram-positive cells with red fluorescence. The kit also includes DAPI to stain the DNA of all bacteria with blue fluorescence. Gram-positive bacteria stained using this kit will show a fluorescent blue interior and fluorescent red cell surface, while gram-negative bacterial will be stained with blue fluorescence only.

Image Unavailable-ENZOBMLAK5180001

FLUOR DE LYS® HDAC8 fluorometric drug discovery kit

Catalog Number: (ENZOBMLAK5180001)
Supplier: ENZO LIFE SCIENCES
Description: A Fluor-de-Lys® fluorescent assay system. The HDAC8 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC8 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions. The Fluor de Lys® HDAC8 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC6. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer produces a fluorophore.
UOM: 1 * 1 KIT
Product Image-TNB-1022-L160

Foxp3/transcription factor staining buffer

Supplier: Tonbo Biosciences
Description: Foxp3/transcription factor staining buffer contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry. This complete kit contains three components for use in these staining protocols for detection of nuclear antigens such as Foxp3 and ROR gamma. Foxp3/transcription factor fix/perm concentrate (4x) 50 ml. A concentrated solution which, when diluted with Foxp3/transcription factor fix/perm diluent (1x) provides best results in protocols for intranuclear staining of transcription factors using fluorescently conjugated antibodies.

Image Unavailable-ENZOBMLUW99150001

Ubiquitin conjugating kit (HeLa lysate-based)

Catalog Number: (ENZOBMLUW99150001)
Supplier: ENZO LIFE SCIENCES
Description: 1. Ubiquitinylation of specific endogenous HeLa lysate proteins, followed by their immediate detection/analysis using antibodies to the protein/s of interest, indicating a particular protein is a substrate for the ubiquitin-proteasome pathway. 2. Ubiquitinylation of proteins of interest contained in exogenously added expression culture/cell extracts or tissue lysates/extracts, followed by their immediate detection/analysis or isolation/purification for use in subsequent experiments. 3. Modification of proteins using ubiquitin derivatives or mutants for improved detection/analysis or investigation of alternative (non-proteasomal) ubiquitin related pathways in subsequent experiments. For example, biotinylated-ubiquitin (Prod. No. BML-UW8705), for sensitive detection
UOM: 1 * 20 Tests
Product Image-2400102.

Cell Viability Reagent Kits for Beckman Coulter Vi-CELL® systems

Supplier: CELL VIABILITY
Description: The Cell Viability Reagent Kits are designed specifically for use with the Beckman Coulter Vi-CELL® Cell Viability Analyser. The Cell Viability Reagent Kits, as well as being cost-efficient, are proven to be of equivalent quality and performance when compared to Beckman Coulter Vi-CELL® Kits.

MSDS Certificates

Image Unavailable-ENZOBMLST3710005

BML-280 ≥98% (by TLC)

Catalog Number: (ENZOBMLST3710005)
Supplier: ENZO LIFE SCIENCES
Description: Potent and selective inhibitor of phospholipase D2 (PLD2) in cellular (PLD1 IC50 = 1900nM Calu-1 cells; PLD2 IC50 = 90nM HEK293-gfpPLD2 cells) and in vitro (hPLD1 IC50 = >20’000nM; hPLD2 IC50 = 500nM) assays.
UOM: 1 * 5 mg
Image Unavailable-ENZOBMLKI1040050

Fluor-de-Lys® Deacetylase Substrate

Catalog Number: (ENZOBMLKI1040050)
Supplier: ENZO LIFE SCIENCES
Description: Fluor de Lys® Substrate is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in a second step, treatment with the Fluor de Lys® Developer produces a fluorophore. Histone deacetylases from all known classes have been successfully assayed with the Fluor de Lys® Substrate. These include human HDACs 1, 2, 3 and 8 (class I), human HDACs 4 and 9 (class II) and the sirtuins yeast Sir2, human SIRT1 and human SIRT2 (class III; reaction must include the sirtuin substrate NAD+). The substrate is cell-permeable and serves as the basis for the HDAC Cellular Activity Assay Kit (Prod. No. BML-AK503). NOTE: Must be used in conjunction with Fluor de Lys® Developer (Prod. No. BML-KI105). Sufficient for 100-1000 assays of HDACs or sirtuins (50 µl reaction volume, 50-500 µM (substrate).
UOM: 1 * 50 µl
Product Image-AATB22906

Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit

Catalog Number: (AATB22906)
Supplier: AAT BIOQUEST
Description: Lipid peroxidation is the oxidative degradation of cellular lipid by reactive oxygen species (ROS).
UOM: 1 * 200 Tests
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CytoScan™-SRB cytotoxicity assay

Catalog Number: (786-213)
Supplier: G-Biosciences
Description: CytoScan™-SRB Cytotoxicity Assay is based upon the quantitative staining of cellular proteins by sulforhodamine B (SRB). The assay is used for cell density determination, based on the measurement of cellular protein content.
UOM: 1 * 1.000 Assays
Product Image-AATB21620

PhosphoWorks™ Fluorimetric ATP Assay Kit

Catalog Number: (AATB21620)
Supplier: AAT BIOQUEST
Description: Adenosine triphosphate (ATP) plays a fundamental role in cellular energetics, metabolic regulation and cellular signaling.
UOM: 1 * 100 Tests
Product Image-AATB21621

PhosphoWorks™ Luminometric ATP Assay Kit, Bright Glow

Supplier: AAT BIOQUEST
Description: Adenosine triphosphate (ATP) plays a fundamental role in cellular energenics, metabolic regulation and cellular signaling.

Product Image-AATB23015

Cell Meter™ Live Cell ATP Assay Kit

Catalog Number: (AATB23015)
Supplier: AAT BIOQUEST
Description: Adenosine triphosphate (ATP) plays a fundamental role in cellular energetics, metabolic regulation and cellular signaling.
UOM: 1 * 100 Tests
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
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The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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