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Image Unavailable-PRSI92-415

Mouse recombinant carboxypeptidase A4

Catalog Number: (PRSI92-415)
Supplier: ProSci Inc.
Description: Carboxypeptidase A4 (CPA4) is a member of the peptidase M14 family. CPA4 is metalloprotease that could be involved in the histone hyperacetylation pathway. CPA4 binds one zinc ion per subunit and could catalyse to release of a C-terminal amino acid, with preference for -Phe, -Leu, -Ile, -Met, -Tyr and -Val.They have distinct expression patterns and different specificities for example, preferentially cleaving aromatic (carboxypeptidase As) or basic (carboxypeptidase Bs) residues. Several, such as carboxypeptidase Xs, have lost their catalytic activity. Carboxypeptidases play important roles in digestion of food, processing of bioactive peptides and blood coagulation.
UOM: 1 * 50 µG
Product Image-APPC252908.1608

Kovac's indole reagent for microbiology

Catalog Number: (APPC252908.1608)
Supplier: PanReac AppliChem
Description: A reagent for the detection of indole-positive and indole-negative microorganisms. Some microorganisms can cleave tryptophan which is especially abundant in trypticalle digested peptone to give pyruvic acid, ammonia and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a colour reaction with 4-dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.
UOM: 1 * 100 mL

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Product Image-STBSHCA3-100M-T150

Healthy human chondrocytes P0 (50-70y), CELLvo™

Supplier: STEMBIOSYS
Description: CELLvo™ Healthy Human Chondrocytes (50-70y) are primary, uncultured cells isolated from recently-diseased cadaver cartilage from donors between 50 and 70 years old. Healthy status is determined by macroscopic inspection of the tissue using a modified Outerbridge (1960) scale in order to distinguish healthy joints from those with signs of osteoarthritis. Cells are isolated by enzymatic digestion prior to cryopreservation.

Image Unavailable-SIAL86339-1G

Sodium taurocholate hydrate ≥97.0% (by TLC), Sigma-Aldrich®

Supplier: Merck
Description: Sodium taurocholate hydrate is used as an emulsifier in <i>in situ</i> preparation of digestive enzymes.

Image Unavailable-ICNA0219398125

Tritirachium album proteinase K, MP Biomedicals

Supplier: MP Biomedicals
Description: Proteinase K is a highly active stable endopeptidase with a broad spectrum of action was isolated by E. Merk's Darmstadt Biochemical Research Department in 1970 from a culture filtrate of the fungus, Tritirachium album Limber. This fungus is able to grow on Keratin (e.g., wool, horn particles) as the sole source of carbon and nitrogen. The isolated protease was, therefore, given the K designation.
Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0,1 to 0,5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

Image Unavailable-ICNA04800259

Agarose

Catalog Number: (ICNA04800259)
Supplier: MP Biomedicals
Description: Low gel temperature electroendosmosis: 0,1 gel strength 1,5% solution: ≥550 gm/cm<sup>2</sup> gel temperature (1%): <lt/>30 °C A 1% solution remains fluid at 37 °C for up to 24 hours. Will set to a firm gel at <lt/>25 °C, and not remelt until temperatures exceed 65 °C. This ability to remain in solution at 30 to 37 °C allows a second digest on a restriction enzyme fragment without need to recover it from the gel. Agarose LGT is also preferred for studies of cellular antibody production and the Jerne Plaque assay of immune responses. Also used in agarose droplet macrophage inhibitory factor (MIF) assay.
UOM: 1 * 100 g
Image Unavailable-PRSI91-750

Human recombinant ERO1-Like alpha (from cells)

Catalog Number: (PRSI91-750)
Supplier: ProSci Inc.
Description: ERO1-Like Protein alpha (ERO1L) is an enzyme that belongs to the EROs family. ERO1L is expressed at high level in esophagus and upper digestive tract. ERO1L is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. ERO1L acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. It associates with ERP44, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. ERO1L may be responsible for a significant proportion of reactive oxygen species (ROS) in the cell. ERO1L responses to temperature stimulus, protein thiol-disulfide exchange, protein folding with or without chaperone cofactor and transport.
UOM: 1 * 50 µG
Product Image-ORIOCOD165

Orion™ AQUAfast COD165 Thermoreactor

Catalog Number: (ORIOCOD165)
Supplier: Thermo Orion
Description: Conduct environmental and wastewater monitoring with the Thermo Scientific™ Orion™ AQUAfast COD165 Thermoreactor. The COD165 thermoreactor is equipped with an automatic voltage selector for voltages between 90 and 260 V with a frequency of 50 or 60 Hz. Select from five preset work temperatures of 100, 120, 150, 160, 165 °C and four preset run times of 30, 60, 120 minutes or continuous time. Simply place 16 mm round sample tubes into the digestor wells, cover with the hinged safety shield, set the temperature and time and let samples digest.
UOM: 1 * 1 items
Product Image-K1081

DreamTaq™ Green PCR Master Mix (2X)

Supplier: Thermo Fisher Scientific
Description: DreamTaq Green PCR Master Mix (2X) is a ready to use solution containing DreamTaq™ DNA polymerase, optimised DreamTaq Green buffer, MgCl<sub>2</sub> and dNTPs. The master mix is supplemented with two tracking dyes and a density reagent that allows for direct loading of the PCR product on a gel. The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications, such as DNA sequencing, ligation and restriction digestion. The master mix retains all features of DreamTaq DNA polymerase. It is capable for robust amplification up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

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Product Image-BE-314

Experimentation kits, mutation detection and analysis

Catalog Number: (BE-314)
Supplier: G-Biosciences
Description: A major challenge for molecular biologists and genetic engineers is to easily detect and analyze genetic mutations that occur naturally, causing diseases, or during genetic engineering or cloning, whether deliberate or accidental. Students learn about different types of genetic mutations, including substitutions, deletions and point mutations and about various techniques used to detect genetic mutations. The kit contains all the reagents to screen simulated clinical samples for a mutant gene, using both the polymerase chain reaction (PCR) and restriction digestion mapping. Students conduct a simple clinical diagnostic experiment in order to identify a diseased patient.
UOM: 1 * 1 KIT
Image Unavailable-K1441

DNA methylation analysis kit (MspI/HpaII), EpiJET™

Catalog Number: (K1441)
Supplier: Thermo Fisher Scientific
Description: 5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression. The EpiJET™ DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyse DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5’-CCGG-3’ tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected. The Epi MspI and Epi HpaII enzymes are specially formulated for epigenetics studies to complete genomic DNA digestion in 1 hour.
UOM: 1 * 1 KIT
Product Image-EP0713

DreamTaq™ Green DNA Polymerase

Supplier: Thermo Fisher Scientific
Description: DreamTaq™ Green DNA Polymerase is a combination of DreamTaq™ DNA Polymerase and 10X DreamTaq™ Green Buffer. DreamTaq™ DNA Polymerase is an enhanced Taq DNA polymerase optimised for high throughput PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase.
DreamTaq™ Green DNA Polymerase incorporates modified nucleotides, but is inhibited by dUTP. The 10X DreamTaq™ Green Buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with PCR performance and is compatible with downstream applications such as DNA sequencing,
phosphorylation, ligation and restriction digestion. For applications that require PCR product analysis by absorbance or fluorescence excitation, it is recommended either to use colourless 10X DreamTaq™ Buffer or purify the PCR product prior to analysis.

Image Unavailable-PRSI33-922

Anti-CELA3B Mouse Monoclonal Antibody [clone: ELTS3B-1]

Catalog Number: (PRSI33-922)
Supplier: ProSci Inc.
Description: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
UOM: 1 * 100 µG

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Product Image-PRSI91-956

Mouse IgG1 Fc Isotype Control

Catalog Number: (PRSI91-956)
Supplier: ProSci Inc.
Description: As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (Fc gamma R ) on phagocytic cells with high affinity.
UOM: 1 * 50 µG
Image Unavailable-PRSI33-782

Anti-CELA3B Mouse Monoclonal Antibody [clone: CELA3B/1257]

Catalog Number: (PRSI33-782)
Supplier: ProSci Inc.
Description: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
UOM: 1 * 100 µG

New Product


Product Image-PRSI49-569

Anti-COL6A1 Rabbit Polyclonal Antibody

Catalog Number: (PRSI49-569)
Supplier: ProSci Inc.
Description: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.
UOM: 1 * 50 µG
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
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