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Food safe kits for identification of pathogenic microorganisms

Supplier: BIOPREMIER
Description: Food safe kits provide a simple, reliable, and rapid procedure for detecting the presence of a specific pathogenic bacteria. The assay is based on 5’ nuclease Real-Time PCR reactions to amplify a unique genomic sequence in the target microorganism. PCR is a method used to amplify a specific DNA sequence which is typically amplified in a reaction containing a thermostable DNA polymerase, nucleotides, and primers complementary to the target sequence. When this solution is heated, the DNA molecule denatures, separating into two strands. As the solution cools, the primers anneal to the target sequences in the separated DNA strands and the DNA polymerase synthesises a new strand by extending the primers with nucleotides, creating a copy of the DNA sequence (amplicons). When repeated, this cycle of denaturing, annealing, and extending exponentially increases the number of target amplicons. In Real-Time PCR, specific fluorescent probes are used to detect the amplified DNA by hybridising with amplicons. These probes are linked to a fluorophore on one end and a quencher that suppresses fluorescence on the other. If the target sequence is present during the PCR, amplification occurs and the probe is degraded, resulting in fluorescence increase. Fluorescence is measured by a detector and the associated software plots the fluorescence intensity versus number of cycles, allowing the determination of the presence or absence of the target organism.

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Cole-Parmer® MP-250 Digital Melting Point Apparatus

Supplier: Cole-Parmer
Description: Rotating viewing head ensures each user can analyse samples clearly.

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PCR plates, 96-well

Supplier: RATIOLAB
Description: These plates conform fully to the highest requirements of PCR. They are made of polypropylene and are characterised by their very thin but uniform walls. In this way, optimal heat transfer in short cycle times is achieved. The smooth inside walls of the cavities minimise the binding of enzymes and nucleic acids.

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PCR plates, 96-well, semi-skirted, flat deck, barcoded

Supplier: Thermo Fisher Scientific
Description: These PP plates are directly compatible with all standard platforms including sequencers with no adapters necessary.

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End point detection system, End-Zone™

Supplier: LABCONCO
Description: End-Zone™ provides an alternative to visual inspection making it easy to determine when freeze drying is complete.

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PCR microtubes with domed cap, Axygen®

Supplier: Corning
Description: PP.

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PCR microtubes

Catalog Number: (731-0407)
Supplier: EPPENDORF
Description: PP.
UOM: 1 * 500 items

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DNA extraction and purification kit

Supplier: Q BIOANALYTIC
Description: Successful PCR applications depend on well purified DNA. In diagnostics and analytics of food and medical samples PCR and real-time PCR has many advantages over conventional methods. In all cases it is of importance to have well purified DNA for subsequent PCR, real-time PCR, hybridization or sequencing purposes. Purified DNA leads to higher sensitivity and also specificity of PCR based tests in difficult matrices. With QuickBlue Extraction and Purification Kit you always get high DNA yields and highly purified DNA. The kit uses paramagnetic nano-particles for purification. It can help to purify DNA from difficult matrices like food or even blood in soil for applications in forensic sciences. DNA in Seawater can also be purified. This method avoids extensive centrifugation steps and can thus be applied in environments where centrifuges are not usable. The kits includes proteinase K as a reagent for cell disruption, so that extraction and purification can be performed with the kit. DNA yield from 1 ml bacterial enrichment is 10 μg of Genomic DNA. The protocol can be adapted to automated magnetic bead separation instruments.

Product Image-731-0015

PCR plates for qPCR, 24-well and 48-well

Supplier: Brand
Description: PP, non skirted. Manufactured under cleanroom conditions, and made from pure, high quality materials (without additives) these PCR plates are suitable for use with most commercially available thermal cyclers.

Image Unavailable-GREI673283_1200

Sapphire Strips of 8 PCR Tube with Snap Strip Dome Cap

Catalog Number: (GREI673283_1200)
Supplier: Greiner Bio-One
Description: Sapphire PCR 8-tube strip, breakable strips, 0,2 ml, with individual snap strip dome cap.
UOM: 1 * 1.200 items

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Product Image-EDVO103-C

Ready-to-Load™ Kits, PCR

Supplier: EDVOTEK
Description: Ready-to-Load™ PCR kit introduces students to the principles and applications of the Polymerase Chain Reaction (PCR). This simulation experiment does not contain human DNA and does not require a thermal cycler.

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PCR tube and Masterclear® cap strips

Supplier: EPPENDORF
Description: Real-time PCR tube strips feature an extremely thin wall for optimal heat transfer. Designed to provide high mechanical stability, they are ideal for real-time PCR applications, especially for use with small reaction volumes. White wells offer improved reflection.

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Strips of 8 PCR tubes with attached flat cap

Catalog Number: (DELT4096.2N)
Supplier: DELTALAB
Description: Made of PP.
UOM: 1 * 125 items
Product Image-731-4008

PCR microtube and cap strips, 0.2 ml, 8-strips, Axygen

Supplier: Corning
Description: Axygen® polypropylene 8/12-tube thin wall PCR tube strips and caps are specifically designed for PCR use and are ideal for thermal transfer applications. The ultra-thin and consistent tube walls ensure that thermal transfer is precisely even along the surface of the tube. The unique cap design guarantees a perfect fit to prevent sample evaporation during thermal cycling. Made from highly polished moulds and free of lubricants, dyes, heavy metals, and fillers. Certified RNase, DNase, and pyrogen free to ensure the purity of your reaction.

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Product Image-732-5595

PCR plates, 384-well, Corning® Thermowell®

Catalog Number: (732-5595)
Supplier: Corning
Description: PP, non sterile. Compatible with a full range of PCR, sequencing and Real-Time PCR equipment.
UOM: 1 * 50 items

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Product Image-211-3382

PCR plates, low profile

Catalog Number: (211-3382)
Supplier: Simport Scientific
Description: PP. Thin walled 96-well PCR plates with small tube volume (100 µl).
UOM: 1 * 100 items
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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