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Supplier: OMEGA BIO-TEK
Description: The E.Z.N.A.® DNA/RNA isolation kit is designed for the simultaneous isolation of both genomic DNA and total RNA from the same cells or tissues. The sample is first lysed and homogenised in a special denaturing buffer, spun to pellet RNA and undigested particles, and then the supernatant is applied to a HiBind® DNA spin column to bind DNA. The RNA pellet is dissolved and purified by a HiBind RNA spin column. Since there is no need to divide the sample into two parts for separate purification procedures, maximum yield of DNA and RNA can be purified from the entire sample.
Supplier: VARIAN
Description: Bond Elut™ LMS polymeric sorbent lets you elute without having to add amine modifiers, buffers, or acids. The elimination of secondary interactions means that elution of analytes can be achieved with pure organic solvents or HPLC mobile phase-compatible solvent mixtures of low ionic strength. These characteristics are crucial to allow compatibility with LC/MS or other delicate analytical techniques.

Supplier: MP Biomedicals
Description: Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Supplier: VWR Collection
Description: Pure cellulose cotton. Suitable for all types of extractors (Soxhlet, Büchi, Gerhardt and more).

Catalog Number: (OXOILP0039B)
Supplier: THERMO FISHER DIAGNOSTICS
Description: Malt extract
UOM: 1 * 500 g

Supplier: Whatman products (Cytiva)
Description: Grade 603 standard cellulose extraction thimbles for DIONEX ASE.

Supplier: Avantor
Description: Solid Phase Extraction (SPE) is commonly used as a highly selective sample clean-up or sample enrichment method prior to subsequent analyses. J.T.Baker® BAKERBOND® SPE 96 well plates are well suited for high throughput and automated SPE methods. With complementary high capacity sorbents to existing spe extraction columns in the J.T.Baker® BAKERBOND® range, higher throughput methodologies can now be simply developed and automated.

Supplier: Lenz Laborglas GmbH & CO.KG
Description: DURAN®, Borosilicate glass.

Catalog Number: (ENZOENZ450130002)
Supplier: ENZO LIFE SCIENCES
Description: Rapid, reliable, high throughput method to isolate histone proteins in mammalian cells for histone modification applications.
UOM: 1 * 1 KIT


Supplier: Thermo Fisher Scientific
Description: B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.

Catalog Number: (ENZOENZ450140100)
Supplier: ENZO LIFE SCIENCES
Description: Rapid, reliable method to isolate histone proteins in mammalian cells and tissues for histone modification applications.
UOM: 1 * 1 KIT


Supplier: Whatman products (Cytiva)
Description: Grade 603 standard cellulose extraction thimbles, made of high quality cellulose, are available in a range of sizes suitable for use in thimble holders and automated extraction apparatus to ensure optimal fit.

Supplier: G-Biosciences
Description: EZ-Grind™ grinding resin for the extraction of both proteins and DNA

Supplier: ROBU GLASFILTER
Description: Borosilicate glass 3.3.

Catalog Number: (537-0028)
Supplier: Whatman products (Cytiva)
Description: High-purity Whatman quartz thimbles from Cytiva support accurate, reproducible emission testing by providing low extractable levels and temperature resistance up to 800 °C.
UOM: 1 * 10 items


Catalog Number: (M04018BF)
Supplier: VWR Chemicals
Description: Rapid method for DNA extraction and purification from all types of microorganisms in a variety of matrices for molecular biology.
UOM: 1 * 1 KIT

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
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