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Product Image-AATB24100

ReadiUse™ Bacterial Cell Lysis Buffer *5X* (10 ml)

Catalog Number: (AATB24100)
Supplier: AAT BIOQUEST
Description: Cell lysis refers to the breaking down of cells, and it is often used to analyze specific cellular compositions such as proteins, lipids, nucleic acids, reporter molecules, cell signal molecules and other small biomolecules.
UOM: 1 * 10 mL
Product Image-786-180

Protein extraction kit, mammalian cell PE LB™

Catalog Number: (786-180)
Supplier: G-Biosciences
Description: Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.
UOM: 1 * 500 mL
Product Image-PIER89802

I-PER™ Insect Cell Protein Extraction Reagents

Catalog Number: (PIER89802)
Supplier: Thermo Fisher Scientific
Description: The I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture. The baculovirus insect cell expression system is an efficient and popular system for production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.
UOM: 1 * 250 mL
Product Image-78840.

Subcellular Protein Fractionation Kit for Cultured Cells

Catalog Number: (78840.)
Supplier: Thermo Fisher Scientific
Description: This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.
UOM: 1 * 1 KIT
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TE buffer, pH 8,1, RNAse free

Supplier: Thermo Fisher Scientific
Description: Trizma is used in the formulation of buffer solutions in the pH range between 7,5 and 8,5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification.

MSDS Certificates

Product Image-786-411

Protein extraction kit, Insect PE LB™

Catalog Number: (786-411)
Supplier: G-Biosciences
Description: Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.
UOM: 1 * 250 mL
Product Image-87790.

Subcellular protein fractionation kit for tissues

Supplier: Thermo Fisher Scientific
Description: This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.

Product Image-PIER78437

Protease inhibitor cocktail, Halt

Supplier: Thermo Fisher Scientific
Description: Halt Protease Inhibitor Cocktail provides all the protection of a broad-spectrum protease inhibitor in a single, ready-to-use liquid format. Halt protects against protein degradation by serine, cysteine, and aspartic acid proteases and aminopeptidases. Metalloproteases are inhibited by the optional use of 0,5 M EDTA. The 100X Halt stock solution makes it easy to dispense the required amount (10 µl per 1 ml) of protease inhibitors into buffers or lysis reagents prior to extraction of proteins from cultured cells, animal tissues, plant tissues, yeast or bacteria. Halt Protease Inhibitor Cocktails are compatible with Thermo Scientific Pierce Protein Extraction Reagents. Halt EDTA-free formulation is ideal for preparing samples that will be analysed by 2-D gel electrophoresis, immobilised metal chelate affinity chromatography (IMAC) and other downstream application where chelating agents interfere.

Product Image-COPA4103CS01

NAO™ Baskets for Rapid and Efficient DNA Recovery

Supplier: Copan
Description: <p>NAO® Basket for rapid and efficient DNA recovery. This is a semi-permeable system designed for releasing and concentrating human DNA from swab samples or other specimens during the extraction step. Lysis in the NAO® Basket: NAO® Basket retains the lysis buffer during the lysis step of the forensic sample.</p>

Product Image-13-2500-02

Peqlab peqGOLD, DNA Clean-up, Gel Extraction Kits

Supplier: VWR Chemicals
Description: Purifies DNA bands 70 bp to 20 kb in length from agarose gels. The DNA band of interest is excised from the gel, dissolved in binding buffer, and efficiently processed in a peqGOLD DNA column. >90% recovery. Elution volume: 30 to 50 µl.

MSDS Certificates

Product Image-786-225

Total protein extraction kit, TPE™

Catalog Number: (786-225)
Supplier: G-Biosciences
Description: Total Protein Extraction (TPE™) Kit is a universal lysis system for the solubilisation of total proteins from animal, plant, yeast, bacteria, and other biological samples. The kit contains ready to use buffers for extracting total protein from cells and tissues for SDS-PAGE analysis.
UOM: 1 * 1 KIT
Product Image-78833.

Nuclear and cytoplasmic extraction reagents, NE-PER

Supplier: Thermo Fisher Scientific
Description: The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

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Mag-Bind® Plant DNA plus kit

Supplier: OMEGA BIO-TEK
Description: The Mag-Bind® Plant DNA 96 plus kit allows rapid and reliable isolation of high-quality genomic DNA from a wide variety of difficult to lyse and high polysaccharide plant species and tissues. The system utilises a CTAB-based lysis buffer without the need for a chloroform or other organic solvent steps. The proprietary wash buffer systems eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. This kit is designed for manual or fully automated high throughput preparation of genomic, chloroplast, and mitochondrial DNA. The Mag-Bind® Plant DNA plus kit also provides greater flexibility than silica plate based systems as partial plates can be used and can be scaled up to a 24-well format with the appropriate magnetic stand. The streamlined protocol can be easily adapted on most robotic liquid handling platforms. Purified DNA is suitable for PCR, qPCR, restriction digestion, hybridisation applications, as well as more demanding applications, such as RAPD.

MSDS

Product Image-PIER90084

Bacterial Protein Extraction Kits, B-PER™

Supplier: Thermo Fisher Scientific
Description: B-PER® Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells. The B-PER® Bacterial Protein Extraction Kit eliminates the need for harsh mechanical procedures, like sonication. The easy-to-use cell lysis solution uses mild non-ionic detergents to disrupt cells and solubilise proteins without denaturation. Enzyme supplements such as DNase I and Lysozyme allow for improved yield of large molecular weight proteins which are difficult to purify. B-PER® is also compatible with downstream protein purification methods such as GST purification and His-tagged immobilised metal affinity chromatography (IMAC).

Image Unavailable-R6840-01

Fungal RNA kit, E.Z.N.A.®

Supplier: OMEGA BIO-TEK
Description: Modified from the E.Z.N.A.® Plant RNA kit, the E.Z.N.A.® Fungal RNA kit uses an improved buffer system that is more efficient for fungal samples. This kit provides convenient and rapid methods for the isolation of total RNA from a variety of fungal species. Rather, the method involves a simple and rapid precipitation step for removal of much of the polysaccharides and phenolic compounds commonly found in fungal tissues. In combination with HiBind® RNA spin columns, this method permits purification of high quality RNA from as much as 200 mg tissue. The system is also efficient enough to purify total RNA from as little as 10 mg of tissue or 100 cells. Purified RNA can be used for many downstream applications such as RT-PCR, northern blotting, Poly(A)+ mRNA purification, and differential display.

MSDS Certificates

Product Image-PIER89895

Pierce™ In-solution Tryptic Digestion and Guanidination Kit

Catalog Number: (PIER89895)
Supplier: Thermo Fisher Scientific
Description: The In-Solution Tryptic Digestion and Guanidination Kit contains proteomics grade trypsin, buffers and reagents to alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry.
UOM: 1 * 1 KIT
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
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The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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