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Catalog Number: (HT5011-1CS)
Supplier: SIGMA ALDRICH MICROSCOPY
Description: Formaldehyde is both a non-coagulant and an additive fixative. Formaldehyde is known to penetrate tissue quickly, but the fixation process may take a long time to form the various cross links with tissue proteins. Formalin fixation is thought to form cross links between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their <i>in vivo</i> relationship. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining. Although there is no true universal fixative, 10% neutral buffered formalin (NBF) is perhaps the most commonly used fixative throughout the world for light microscopy and is a somewhat forgiving fixative. Of the dozens and dozens of available fixatives, it is hard to imagine a fixative more commonly used than 10% NBF. 10% neutral buffered formalin is well suited for large throughput laboratories, and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue.
UOM: 1 * 1 Each


Catalog Number: (HT501640-19L)
Supplier: SIGMA ALDRICH MICROSCOPY
Description: Formalin solution, neutral buffered, 10% is the most commonly used fixative for light microscopy. It requires a relatively short period of fixation. It is suitable for the long-term storage of tissue. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining. 10% NBF is well suited for large throughput laboratories, and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their <i>in vivo</i> relationship.
UOM: 1 * 19 L


Catalog Number: (ROCKMB-013)
Supplier: Rockland Immunochemicals
Description: Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.
UOM: 1 * 1 L

Catalog Number: (HT501128-4L)
Supplier: SIGMA ALDRICH MICROSCOPY
Description: (= 10% Formaline solution)
UOM: 1 * 4 L

MSDS


Catalog Number: (RS2-900ML)
Supplier: SIGMA ALDRICH MICROSCOPY
Description: Intended for use as a buffer with various Romanowky type staining procedures, specifically those performed on the Hematek. RS2 is a component of Sigma products WGHT, WSHT and WSHT-S.
UOM: 1 * 900 mL


Catalog Number: (B3269-5ML)
Supplier: SIGMA ALDRICH MICROSCOPY
Description: Bromophenol Blue–Xylene Cyanol Dye solution has been used in the formamide loading buffer for the size purification of ligated RNA from polyacrylamide gel.
UOM: 1 * 5 mL


Catalog Number: (854C-20ML)
Supplier: SIGMA ALDRICH MICROSCOPY
Description: Citrate Concentrated Solution. pH approx. 3.0 when diluted 1:50. For the preparation of a 60% citrate buffered acetone solution used as a fixative for blood smears.
UOM: 1 * 20 mL


Catalog Number: (854-200ML)
Supplier: SIGMA ALDRICH MICROSCOPY
Description: Citrate Solution for the preparation of a 60% citrate buffered acetone solution used as a fixative for blood smears. No dilution required. Add 30 ml citrate solution to 20 ml absolute acetone for preparation of fixative.
UOM: 1 * 200 mL


Supplier: SIGMA ALDRICH MICROSCOPY
Description: Certified for Giemsa stain for blood films and for the Wolbach′s Giemsa method for paraffin sections. Giemsa is a versatile polychromatic stain, which is suitable for staining a diverse range of specimens. It is a buffered thiazine-eosinate solution designed to provide coloration of blood cells. Giemsa’s stain is frequently used for diagnostic purposes in hematology to differentiate the nuclear and cytoplasmic morphology of platelets, RBCs, WBCs, and parasites. It is frequently used in combination with other dye solutions as May-Grunwald’s solution for Pappenheim (MGG) and Wright-Giemsa. The Giemsa stain was adapted to histology due to its unique staining of chromatin, nuclear membranes, and cytoplasmic elements. Giemsa′s staining solution is composed of methylene blue, azure, and eosin.

Supplier: SIGMA ALDRICH MICROSCOPY
Description: Amaranth has been used as a dye for the microscopic visualization of crop contraction in blowflies. It has been used in the electrophoresis gel to track the migration front of the electrode buffer.

Supplier: SIGMA ALDRICH MICROSCOPY
Description: Histo/Zyme is a stabilized proteolytic enzyme in buffered solution, used to unmask immunoreactive sites altered by fixation and/or the embedding process. Treatment with a proteolytic enzyme in immunohistochemistry (IHC) is required to digest excess linkages and expose the antigen. The use of Histo/Zyme results in superior staining over traditionally used enzyme pretreatments, such as pepsin and trypsin. Moreover, Histo/Zyme requires only a brief incubation at room temperature. Performing antigen recovery with Histo/Zyme will allow you to dilute the primary antibody 5 to 10 fold further and achieve optimal staining.

Supplier: SIGMA ALDRICH MICROSCOPY
Description: Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The tracking dye typically migrates with the DNA molecules around 5 kb. Xylene cyanol may also be used in loading buffer that facilitates the observation of the sample during gel loading and electrophoresis.

Supplier: SIGMA ALDRICH MICROSCOPY
Description: Bromophenol blue has been used in the sample buffer prepared for western blotting. A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

Supplier: SIGMA ALDRICH MICROSCOPY
Description: Bromothymol blue has been used for freshwater pH measurements over a broad range of buffer intensities.

Catalog Number: (STMC100-1451)
Supplier: STEMCELL Technologies
Description: Ideal for the permeabilisation of fixed cells during labeling of cytokines and other cytoplasmic antigens. Intracellular Permeabilisation Buffer (10X) is uniquely formulated for optimal resolution and to reduce background and is suitable for use with fluorochrome-conjugated antibodies for flow cytometry.
UOM: 1 * 150 mL

New Product


Catalog Number: (STMC100-1450)
Supplier: STEMCELL Technologies
Description: Ideal for fixing cells prior to permeabilisation, fixation buffer can be used with Intracellular Permeabilisation Buffer (10X) to conduct intracellular staining for flow cytometry. This fixation buffer can also be used to preserve fluorescently labeled cells prior to analysis by flow cytometry.
UOM: 1 * 100 mL

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
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This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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