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Product Image-EDVO110

Experimentation kits, molecular weight determination of proteins (agarose-based)

Catalog Number: (EDVO110)
Supplier: EDVOTEK
Description: This experiment introduces a simple method to determine protein subunit molecular weights using horizontal electrophoresis. As the protein standards and 'unknowns' are prestained, the separation of proteins can be observed during electrophoresis. Included in the experiment is protein-grade agarose, which provides an alternative to the use of polyacrylamide gels.
UOM: 1 * 1 KIT
Product Image-BE-312

Experimentation kits, expression of a recombinant protein

Catalog Number: (BE-312)
Supplier: G-Biosciences
Description: The final goal in molecular biology is often the expression of a recombinant protein. The transformed plasmids can be used as templates by the bacteria to produce protein. Students learn about essential promoters and other elements necessary for successful protein expression in bacteria, including the differences between inducible and constitutive (unregulated) expression.The Expression of a Recombinant Protein kit allows students to express a protein either constitutively or under the control of an inducible promoter, which is activated with IPTG (isopropyl-beta-D-thiogalactopyranoside).
UOM: 1 * 1 KIT
Product Image-BE-301

Experimentation kits, bacterial conjugation

Catalog Number: (BE-301)
Supplier: G-Biosciences
Description: Bacterial conjugation is a naturally occurring process that allows the transfer of DNA from one bacterium to another, which allows the transfer of genetic traits, particularly drug resistance. The kit contains two bacteria with different drug resistance genes and all the tools for students to study bacterial conjugation. This kit teaches the difference between bacterial genomic DNA and the transferable plasmid DNA and the mechanisms of bacterial conjugation. Students will also learn important basic microbiological techniques, including bacterial growth in liquid broth and on solid agar plates, antibiotic selection of bacteria and important aseptic techniques.
UOM: 1 * 1 KIT
Product Image-EDVO243

Experimentation kits, ion exchange chromatography

Catalog Number: (EDVO243)
Supplier: EDVOTEK
Description: Most molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically. In this experiment, a mixture of two chemicals is absorbed onto a solid support ion-exchange column and separated during elution under conditions that influence their net charge.
UOM: 1 * 1 items

MSDS


Product Image-PRSIXPS-1009

Anti-MAPK1 Rabbit Polyclonal Antibody

Catalog Number: (PRSIXPS-1009)
Supplier: ProSci Inc.
Description: Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory. The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204.
UOM: 1 * 100 µl
Product Image-BE-318

Experimentation kits, nucleic acid quantification

Catalog Number: (BE-318)
Supplier: G-Biosciences
Description: The kit utilises the principle of diffusion of nucleic acids on a nylon membrane to determine their concentration. No spectrophotometers required.
UOM: 1 * 1 KIT
Product Image-763-0001

Experimentation kits, amplification of DNA by PCR

Catalog Number: (763-0001)
Supplier: EDVOTEK
Description: In this PCR experiment, students make billions of copies of a small amount of DNA in only 90 minutes. They just need to mix template DNA and primers with PCR beads that contain all of the other components required to carry out a PCR reaction. Students see the increasing amounts of DNA for themselves, taking samples every few cycles and analysing them on a DNA gel.
UOM: 1 * 1 KIT
Product Image-BE-403

Experimentation kits: biodiversity study and biomass analysis

Catalog Number: (BE-403)
Supplier: G-Biosciences
Description: Biodiversity lab activity is designed for determination of protein contents and biomass in diverse biological samples to study how biomass is related to biodiversity in nature. Students collect and catalogue plant leaf samples from a diverse group of locally available plants. This lab activity involves determination of natural weight of each plant sample, grinding a predetermined amount of each sample, and the subsequent extraction of proteins from the samples. Students then learn to determine protein contents of each plant sample and attempt to relate the protein content with biomass. Students in this lab activity are challenged to think, analyse, and seek answers as to why protein biomasses vary for a given natural weight for different plants. Finally, they will relate that finding to the biodiversity of nature.
UOM: 1 * 1 KIT
Image Unavailable-BOSSBS-10138R-A680

Anti-Eph receptor B1 Tyr928 Rabbit Polyclonal Antibody (Alexa Fluor® 680)

Catalog Number: (BOSSBS-10138R-A680)
Supplier: Bioss
Description: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional Signalling into neighboring cells. The Signalling pathway downstream of the receptor is referred to as forward Signalling while the Signalling pathway downstream of the ephrin ligand is referred to as reverse Signalling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK Signalling cascades to regulate cell migration and adhesion respectively.
UOM: 1 * 100 µl
Product Image-EDVO253

Experimentation kits, diversity of fish proteins

Catalog Number: (EDVO253)
Supplier: EDVOTEK
Description: In this experiment, students study the diversity of fish using pre-stained, lyophilised proteins. Total protein from perch, walleye and salmon is extracted and pre-stained using an indicator dye. Each fish protein sample has a characteristic banding pattern when separated by denaturing SDS-polyacrylamide gel electrophoresis, which can be used to identify the specific species.
UOM: 1 * 1 KIT
Image Unavailable-BOSSBS-10138R-A750

Anti-Eph receptor B1 Tyr928 Rabbit Polyclonal Antibody (Alexa Fluor® 750)

Catalog Number: (BOSSBS-10138R-A750)
Supplier: Bioss
Description: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional Signalling into neighboring cells. The Signalling pathway downstream of the receptor is referred to as forward Signalling while the Signalling pathway downstream of the ephrin ligand is referred to as reverse Signalling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK Signalling cascades to regulate cell migration and adhesion respectively.
UOM: 1 * 100 µl
Product Image-BE-203

Experimentation kits, antibiotic sensitivity and bacteria screening

Catalog Number: (BE-203)
Supplier: G-Biosciences
Description: Antibiotics play a crucial role in the manipulation, screening and killing of bacteria in a range of biotechnology processes. This kit specifically teaches the basic principles of antibiotics, bacterial resistance and susceptibility. Students learn and understand the use of antibiotic resistance in screening for infectious diseases. Utilising a bacterial strain, students learn the effects of different antibiotics and visualise bacterial sensitivity and resistance to the supplied antibiotics. This method involves the use of filter paper discs impregnated with a specified concentration of antibiotics on the surface of an agar plate containing microbial cells. This kit will enable students to analyse the inhibitory effects of different antibiotics on selected bacterial cells and then determine which antibiotic is the most suitable to treat a bacterial infection.
UOM: 1 * 1 KIT
Product Image-EDVO908

Experimentation kits, introduction to plant cell culture

Catalog Number: (EDVO908)
Supplier: EDVOTEK
Description: Genetic modification of plants is a controversial area of biotechnology. All such experiments in plants begin with establishing plant cells in culture. This involves de-differentiating plant cells to form plant 'stem cells'. In this experiment, students establish cell cultures of African violets from leaves. They then use plant growth regulators to encourage root growth from the cultured cells, and produce a mature plant.
UOM: 1 * 1 KIT
Product Image-EDVO956

Experimentation kit, bioremediation by oil eating bacteria

Catalog Number: (EDVO956)
Supplier: EDVOTEK
Description: Oil spills cause devastation to the environment, killing sea life, birds, and coastal plants. Spraying areas of contamination with oil-eating microbes accelerates the degradation of the oil. This process is known as bioremediation. In this open-ended experiment, students grow a mixture of oil-eating bacteria and observe their effectiveness at degrading a variety of oils.
UOM: 1 * 1 KIT

MSDS


Product Image-EDVO315

Kits: in search of the sickle cell gene by southern blot, Ready-to-Load™

Catalog Number: (EDVO315)
Supplier: EDVOTEK
Description: Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane the method allows the precise analysis and identification of DNA bands on a gel. Using this kit, students use Southern blotting to find a point mutation in the haemoglobin gene indicating Sickle Cell Anaemia.
UOM: 1 * 1 items

MSDS


Product Image-PRSI50-207

Anti-MAPK1 Rabbit Polyclonal Antibody

Catalog Number: (PRSI50-207)
Supplier: ProSci Inc.
Description: Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002; Ahn, 1993; Tanoue and Nishida, 2003; Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).
UOM: 1 * 100 µl
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
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This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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