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Product Image-EDVO105-C

Kits: mapping of restriction sites on plasmid DNA, Ready-to-Load™

Supplier: EDVOTEK
Description: DNA mapping is a common procedure used to determine the location of genes. In this experiment, DNA markers and pre-digested plasmid DNA fragments are mapped using agarose gel electrophoresis.

Product Image-EDVO115-C

Kits: family pedigree cancer gene detection, Ready-to-Load™

Supplier: EDVOTEK
Description: In this experiment, students determine a pedigree for a family thought to be carriers of a mutation in their p53 genes. This is followed by a diagnostic agarose gel analysis to diagnose the state of the p53 gene in individual family members.

Product Image-PRSIXPS-1009

Anti-MAPK1 Rabbit Polyclonal Antibody

Catalog Number: (PRSIXPS-1009)
Supplier: ProSci Inc.
Description: Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory. The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204.
UOM: 1 * 100 µl
Product Image-EDVOS-30

Experimentation kits, how clean is the water we drink and the air we breathe?

Catalog Number: (EDVOS-30)
Supplier: EDVOTEK
Description: With this kit, students sample water and air and then grow any microbes present overnight. A safe and simple way to teach pollution.
UOM: 1 * 1 items
Product Image-EDVO117-C

Kits: detection of mad cow disease, Ready-to-Load™

Supplier: EDVOTEK
Description: Bovine spongiform encephalophathy (BSE), also known as Mad Cow Disease, is a neurodegenerative, fatal condition in cattle. Consuming BSE-infected beef is believed to be the cause of a similar condition in humans, Creutzfeldt-Jakob disease. In this experiment, students examine simulated PCR products from several feed mills, to determine any possible violations of the ban on including animal parts in cattle feed.

Image Unavailable-ICNA0215127080

Hyaluronidase, MP Biomedicals

Supplier: MP Biomedicals
Description: Unit Definition: Enzyme activity is determined by a change in optical density in a solution of hyaluronic acid. Activity is compared to a hyaluronidase International Standard and is expressed as an International Unit (U).

Product Image-BE-203

Experimentation kits, antibiotic sensitivity and bacteria screening

Catalog Number: (BE-203)
Supplier: G-Biosciences
Description: Antibiotics play a crucial role in the manipulation, screening and killing of bacteria in a range of biotechnology processes. This kit specifically teaches the basic principles of antibiotics, bacterial resistance and susceptibility. Students learn and understand the use of antibiotic resistance in screening for infectious diseases. Utilising a bacterial strain, students learn the effects of different antibiotics and visualise bacterial sensitivity and resistance to the supplied antibiotics. This method involves the use of filter paper discs impregnated with a specified concentration of antibiotics on the surface of an agar plate containing microbial cells. This kit will enable students to analyse the inhibitory effects of different antibiotics on selected bacterial cells and then determine which antibiotic is the most suitable to treat a bacterial infection.
UOM: 1 * 1 KIT
Product Image-EDVO956

Experimentation kit, bioremediation by oil eating bacteria

Catalog Number: (EDVO956)
Supplier: EDVOTEK
Description: Oil spills cause devastation to the environment, killing sea life, birds, and coastal plants. Spraying areas of contamination with oil-eating microbes accelerates the degradation of the oil. This process is known as bioremediation. In this open-ended experiment, students grow a mixture of oil-eating bacteria and observe their effectiveness at degrading a variety of oils.
UOM: 1 * 1 KIT

MSDS


Product Image-EDVO255

Purification and size determination of green and blue fluorescent proteins

Catalog Number: (EDVO255)
Supplier: EDVOTEK
Description: When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP are used as an assay during their purification from an <i>E. coli</i> extract. The column fractions containing GFP or BFP are identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.
UOM: 1 * 1 KIT
Product Image-EDVO316

Experimentation kits, in search of the cholesterol gene

Catalog Number: (EDVO316)
Supplier: EDVOTEK
Description: Coronary heart disease and stroke are major causes of death in the Western world. Elevated blood cholesterol levels are a serious risk factor in both conditions. The genetic disease familial hypersholesterolemia (FH) causes an increase in blood levels of the bad form of cholesterol, low density lipoprotein (LDL). In untreated patients with the mutant FH gene, the condition can cause premature death. This experiment introduces the colorimetric enzymatic reaction which is the basis of the clinical cholesterol test. In addition, using agarose gel electrophoresis, students can analyse a simulated genetic screening for a disease.
UOM: 1 * 1 KIT
Image Unavailable-ENZOADIKAPCA002D

Anti-CAMK2 Rabbit Polyclonal Antibody

Supplier: ENZO LIFE SCIENCES
Description: CaMKII (calmodulin-dependent kinase II) is an enzyme which is activated by increases in intracellular Ca2+ ion concentration and it has been proposed to be pivotal in regulating synaptic strength and maturation of synapses during development.  This process is thought to be critical in memory and learning and in establishing the specificity of synaptic connections. There are over two dozen alternative splice variants of CaMKII which are encoded by four genes, a, b, g, and d with apparent molecular masses of 50-60 kDa.  CaMKII is widely distributed in many tissues, but is highly expressed in brain. Several studies demonstrate that CaMKII is a multifunctional enzyme which modulates the synaptic strength by binding to a subunit of NMDA receptors and promoting the phosphorylation of this NMDA receptor subunit.  CaMKII also regulates DLG localization at synapses by co-localization with DLG in the same protein complex.  Experimental data suggest that CaMKII is critically involved in the development of morphine tolerance as well as dependence and inhibition of this enzyme may have some therapeutic benefit in the treatment of opiate tolerance and dependence.  It also has been demonstrated that d CaMKII isozyme is down-regulated in human tumor cells indicating a role for d CaMKII isozymes in cellular differentiation.  Changes in d CaMKII isozyme expression pattern in human hearts during heart failure suggest that CaMKII is important for regulation of heart function.  This immunoaffinity purified antibody detects proteins of 50-60 kDa, corresponding to apparent molecular mass of CaMKII isoforms on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, hamster, guinea pig, chicken and rabbit origins. Recombinant rat calmodulin-dependent protein kinase II a subunits expressed in sf9 insect cells are also detected. As the sequences of the rat a, d, and g isoforms are conserved over this amino terminus region, this antibody is expected to recognize the d, g and a isoforms. This antibody is not expected to cross-react with the b isoform. Proteins of unknown identity, ~40, 45 and 90 kDa, may be detected on immunoblot analysis with some lysates.

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Product Image-EDVO203

Experimentation kits, isolation of E. coli Chromosomal DNA

Catalog Number: (EDVO203)
Supplier: EDVOTEK
Description: Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA extraction LyphoCells and reagents for isolating chromosomal DNA from <i>E. coli</i>. After spooling from solution, the DNA can be dissolved and analysed by agarose gel electrophoresis as an optional lab extension activity.
UOM: 1 * 1 KIT
Product Image-EDVO222

Transformation with blue and green fluorescent proteins

Catalog Number: (EDVO222)
Supplier: EDVOTEK
Description: The Green Fluorescent Protein (GFP) from the jellyfish <i>Aequorea victoria</i> is used extensively in all areas of science. Many organisms have been transformed with the GFP gene, the gene responsible for bioluminescence in jellyfish. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment, students transform bacteria either by GFP, BFP or both.
UOM: 1 * 1 items

MSDS


Product Image-PRSI50-207

Anti-MAPK1 Rabbit Polyclonal Antibody

Catalog Number: (PRSI50-207)
Supplier: ProSci Inc.
Description: Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002; Ahn, 1993; Tanoue and Nishida, 2003; Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).
UOM: 1 * 100 µl
Product Image-PRSIXPS-1012

Anti-GRIA1 Rabbit Polyclonal Antibody

Catalog Number: (PRSIXPS-1012)
Supplier: ProSci Inc.
Description: The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by beta-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct subunits GluR1-4 and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is also potentiated by phosphorylation at Ser831. In addition, phosphorylation of this site has been linked to synaptic plasticity as well and learning and memory.
UOM: 1 * 150 µl
Image Unavailable-DYLO8551-3224

Osteometric board

Catalog Number: (DYLO8551-3224)
Supplier: DYNALON
Description: Made of durable, lightweight polypropylene, this osteometric board is the most affordable on the market.
UOM: 1 * 1 items
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
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The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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