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Ubiquitin conjugating kit (HeLa lysate-based)

Catalog Number: (ENZOBMLUW99150001)
Supplier: ENZO LIFE SCIENCES
Description: 1. Ubiquitinylation of specific endogenous HeLa lysate proteins, followed by their immediate detection/analysis using antibodies to the protein/s of interest, indicating a particular protein is a substrate for the ubiquitin-proteasome pathway. 2. Ubiquitinylation of proteins of interest contained in exogenously added expression culture/cell extracts or tissue lysates/extracts, followed by their immediate detection/analysis or isolation/purification for use in subsequent experiments. 3. Modification of proteins using ubiquitin derivatives or mutants for improved detection/analysis or investigation of alternative (non-proteasomal) ubiquitin related pathways in subsequent experiments. For example, biotinylated-ubiquitin (Prod. No. BML-UW8705), for sensitive detection
UOM: 1 * 20 Tests

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Product Image-MLDVR8044

Cyclic-AMP fluorescent assay kits, CatchPoint™

Supplier: Molecular Devices
Description: CatchPoint™ cAMP Fluorescent Assay Kit measures cAMP levels via a competitive immunoassay format. Cell signaling via G protein-coupled receptors (GPCRs) can be assessed by monitoring the downstream effectors calcium or Cyclic AMP (cAMP). Monitoring levels of cAMP, a second messenger produced in response to activation of adenylate cyclase, is one of the most common ways to screen for agonists and antagonists of GPCRs.

Product Image-ROCKPA00-02

Avidin, biotin, protein A/G and streptavidin reagents

Supplier: Rockland Immunochemicals
Description: Avidin is biotin-binding protein, tetramer and suitable for used as a antigen, control and standard in assays. Avidin fluorescein, rhodamine, alkaline phosphatase, Texas Red™ conjugated are suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent Western blotting. Avidin conjugated with glucose oxidase and peroxidase are useful as a detection reagent for primary antibodies conjugated with biotin and also can be utilised in Western blotting and ELISA experiments with proper substrates.

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Green detection kit, LYSO-ID®

Supplier: ENZO LIFE SCIENCES
Description: LYSO-ID® Green Detection Kit is an acidic organelle-selective dye specially designed to use with RFP-expressing cell lines, as well as cells expressing blue, cyan or orange fluorescent proteins (BFPs, CFPs, OFPs).

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Product Image-ENZOBMLUW09700001

Auto-Ubiquitinylation Kit

Catalog Number: (ENZOBMLUW09700001)
Supplier: ENZO LIFE SCIENCES
Description: This E3 ligase auto-ubiquitinylation kit enables proteins to be tested for ubiquitin E3 ligase activity through assessment of their ability to under auto-ubiquitinylation. Utilizing the first three steps in the ubiquitin cascade the kit facilitates ubiquitinylation of known or putative E3 ligase enzymes followed by Western blot analysis using the highly sensitive reagents provided or using antibodies to the specific protein of interest (user supplied). A high integrity ubiquitin E3 ligase enzyme is also provided for use as a positive control.
UOM: 1 * 10 Tests

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Product Image-ENZOENZ510050100

Red detection kit (GFP-CERTIFIED®), LYSO-ID®

Catalog Number: (ENZOENZ510050100)
Supplier: ENZO LIFE SCIENCES
Description: LYSO-ID® Red Detection Kit (GFP-CERTIFIED®) is an acidic organelle-selective dye for live cell staining of lysosomes. It is an ideal choice for fluorescence co-localisation imaging with GFP-tagged proteins and providing spectrally pure red signal.
UOM: 1 * 100 Tests

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Bovine serum albumin (BSA)

Catalog Number: (ENZOADI801928)
Supplier: ENZO LIFE SCIENCES
Description: This BSA Solution (10%) is specially designed to create the Blocking Buffer and Assay Buffer in ImmunoSet products. Each bottle is sufficient for use with 5×96 wells microtiter plates if following the instructions in our ImmunoSet protocol. The BSA Solution may be used for any other assay development needs. Additional assay development reagents are available as companion products to our ImmunoSet products.
UOM: 1 * 50 mL

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Product Image-CAYM10012445-96

His-Tag detection ELISA kit

Supplier: Cayman Chemical
Description: A competitive assay for rapid screening for His-tagged proteins.

Image Unavailable-ENZOBMLP2660001

CDK2 substrate,Biotin

Catalog Number: (ENZOBMLP2660001)
Supplier: ENZO LIFE SCIENCES
Description: This peptide, Biotin-Axh-HHASPRK, is a specific substrate for CDK2. The biotin allows the peptide to be used in kinase assays with streptavidin-bound membranes.
UOM: 1 * 1 mg

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Poly-Ubiquitin chains (linear)

Catalog Number: (ENZOBMLUW08250001)
Supplier: ENZO LIFE SCIENCES
Description: For use in deubiquitinylating enzyme assays and linear polyubiquitin binding studies.
UOM: 1 * 1 Each

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Image Unavailable-APOSBIMI1441-5G

Soybean trypsin inhibitor

Supplier: Apollo Scientific
Description: Assay >10000 U/mg lyophilizedInhibitor of Trypsin, Chymotrypsin, Plasmin and otherproteolytic enzymes.

Image Unavailable-ENZOENZ51007500

Red detection kit (GFP-CERTIFIED®), MITO-ID®

Supplier: ENZO LIFE SCIENCES
Description: MITO-ID® Red Detection Kit (GFP-CERTIFIED®) is a photostable, non-toxic and selective mitochondrial dye that stains live, detergent permeabilised and aldehyde fixed cells. This kit is specifically designed for use with GFP expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFP's, CFP's, YFP's).

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Lipofuscin autofluorescence quencher, TrueBlack™

Catalog Number: (23007.)
Supplier: Biotium
Description: TrueBlack™ is a reagent for quenching lipofuscin autofluorescence in tissue sections for immunofluorescence staining. Lipofuscin consists of highly autofluorescent granules of oxidized proteins and lipids that build up in the lysosomes of aging cells. Lipofuscin granules fluoresce brightly in all channels used for fluorescence microscopy and accumulate in a cell and tissue types with age.
UOM: 1 * 1 mL

MSDS


Image Unavailable-ENZOBMLAW97850005

Ac-Arg-Leu-Arg-AMC

Catalog Number: (ENZOBMLAW97850005)
Supplier: ENZO LIFE SCIENCES
Description: This fluorogenic peptide is often the substrate of choice for assaying the trypsin-like activity of purified proteasomes. Benefits include a low Km, high specific activity, and the fact that the substrate is cleaved exclusively at the R-AMC bond.
UOM: 1 * 5 mg

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Caspase fluorometric substrate set

Catalog Number: (ENZOALX850229KI01)
Supplier: ENZO LIFE SCIENCES
Description: Fluorometric substrates for assaying activities of members of caspase family proteases. All substrates are provided in liquid ready-to-use form.
UOM: 1 * 1 SET

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Ubiquitin activating kit

Catalog Number: (ENZOBMLUW0400A0001)
Supplier: ENZO LIFE SCIENCES
Description: Ubiquitin Activating Kit for activation of ubiquitin for use in ubiquitinylation experiments. The kit provides the means of generating thioester linked, activated ubiquitin-E1 conjugates, utilising the first step in the ubiquitin cascade, for investigation of ubiquitin activation, subsequent ubiquitin transfer to/interaction with E2 conjugating enzymes, and their use in the ubiquitinylation of E3 ligases and target substrate proteins. Ubiquitin antibody provided allows high sensitivity detection of ubiquitin and ubiquitin-conjugates.
UOM: 1 * 20 Tests

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
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The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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