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You Searched For: Protein+Extraction+Reagents


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Product Image-M334-100ML

VWR® Lysis Buffer, Hypotonic

Catalog Number: (M334-100ML)
Supplier: VWR Chemicals
Description: Detergent-free buffer for isolation of proteins from tissue culture cells. Cell lysis and the subsequent isolation of the crude cytoplasmic fraction completed in less than 30 m.
UOM: 1 * 100 mL
Product Image-PIER90051

Pierce™ EndoProteinase Lys-C Protease, MS Grade

Catalog Number: (PIER90051)
Supplier: Thermo Fisher Scientific
Description: Endoproteinase Lys-C protease is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.
UOM: 1 * 20 µG
Product Image-DGA01

Optimizer blueBALLS™

Catalog Number: (DGA01)
Supplier: G-Biosciences
Description: The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.
UOM: 1 * 500 items
Image Unavailable-786-214

Detergent removal system, DetergentOUT™ Tween®

Supplier: G-Biosciences
Description: DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.

Certificates

Product Image-PIER88702

Universal nuclease for cell lysis, Pierce™

Supplier: Thermo Fisher Scientific
Description: The Pierce™ Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.

Product Image-786-257

FOCUS™ Yeast proteome kits

Catalog Number: (786-257)
Supplier: G-Biosciences
Description: Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilises nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™ Zymolyase®, which has improved stability and shelf life. Enzymatic action is followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
UOM: 1 * 1 KIT
Product Image-PIER90054

Endoproteinase Glu-C protease, MS grade, Pierce™

Catalog Number: (PIER90054)
Supplier: Thermo Fisher Scientific
Description: Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.
UOM: 1 * 50 µG

MSDS


Product Image-PIER90053

Endoproteinase Asp-N protease, MS grade, Pierce™

Catalog Number: (PIER90053)
Supplier: Thermo Fisher Scientific
Description: Asp-N protease is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.
UOM: 1 * 2 µG

MSDS


Product Image-PIER90056

Endoproteinase chymotrypsin protease (TLCK treated), MS grade, Pierce™

Catalog Number: (PIER90056)
Supplier: Thermo Fisher Scientific
Description: Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.
UOM: 1 * 100 µG
Product Image-786-128

Detergent removal systems, OrgoSol DetergentOUT™

Supplier: G-Biosciences
Description: OrgoSol DetergentOUT™ is suitable for removal of detergents from protein solutions, including hydrophobic protein solutions. Compatible with all detergent types, its performance is not dependent on the concentration of detergents in the solution. It is highly flexible and can be used to process small and large sample volumes.

Product Image-786-077

Protein reducing agents

Supplier: G-Biosciences
Description: Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

Product Image-786-258

Proteome kits, FOCUS™ Bacterial Proteome

Catalog Number: (786-258)
Supplier: G-Biosciences
Description: Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilises nearly all of the proteins from <i>E.coli</i>, including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme enzyme, followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
UOM: 1 * 1 KIT
Product Image-786-599

Immobilised Iminobiotin Resin

Catalog Number: (786-599)
Supplier: G-Biosciences
Description: Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.
UOM: 1 * 5 mL
Image Unavailable-ENZOBMLKI1050300

Fluor-de-Lys® Developer I concentrate

Catalog Number: (ENZOBMLKI1050300)
Supplier: ENZO LIFE SCIENCES
Description: FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).
UOM: 1 * 300 µl

New Product


Product Image-786-247

Protein fractionation kit, FOCUS™ soluble and insoluble

Catalog Number: (786-247)
Supplier: G-Biosciences
Description: FOCUS™ Soluble & Insoluble is a complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a strong chaotropic extraction buffer to solubilise difficult proteins.
UOM: 1 * 1 KIT
Product Image-786-580

Gram Negative lysis buffer

Supplier: G-Biosciences
Description: Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including <i>E.coli</i> strains.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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