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Affinity protein expression and purification systems

Catalog Number: (AGLS214303)
Supplier: AGILENT
Description: The affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification.
UOM: 1 * 10 mL
Product Image-786-803

Antibody clean up kit, Pearl™

Catalog Number: (786-803)
Supplier: G-Biosciences
Description: The Pearl™ Antibody Clean Up Kit is designed for the rapid clean up of antibody solutions using a combination of our Pearl™ IgG Purification Resin to remove the protein stabilisers and SpinOUT™ desalting columns to ensure the antibody solutions are in an optimal buffer for clean up. It binds high abundant, non IgG proteins (i.e. BSA and gelatin) and allows the IgG molecules to pass through in a physiological buffer. For the purification of ten 0,5 ml IgG samples with up to 1% BSA and gelatin.
UOM: 1 * 1 KIT
Product Image-786-809

Carboxyl coupling resin

Supplier: G-Biosciences
Description: Carboxyl coupling resin consists of 6% cross-linked agarose with covalently linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. Molecules, including proteins, peptides and nucleic acids, are covalently coupled to the free primary amines, and the stable columns are ideal for affinity purification of antibodies and other interacting partners.

Product Image-786-218

Concanavalin A agarose

Supplier: G-Biosciences
Description: Concanavalin A (Con A) agarose consists of Con A coupled to 6% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from <i>Canavalia ensiformis</i> (jack bean). It is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. It has also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles. As stated above, it is a metalloprotein and to maintain its binding characteristics the presence of both Mn²⁺ and Ca²⁺ is essential. Each subunit of Con A utilises one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate-binding activity.

Product Image-786-271

Immobilisation kit, SDC™ (Steroid/Drug/Compound)

Catalog Number: (786-271)
Supplier: G-Biosciences
Description: SDC™ (Steroid/Drug/Compound) Immobilisation Kit is designed for the immobilisation of steroids, drugs and chemical compounds that lack primary amines, sulfhydryls, carbonyls and other common coupling groups to a solid-phase agarose support for the use in affinity purification. The kit uses Immobilised DADPA (diaminodipropylamine) resin to bind steroids, drugs and chemicals through their active hydrogens.
UOM: 1 * 1 KIT
Image Unavailable-46488.A1

Ion exchange resins, Diaion® HP20

Catalog Number: (46488.A1)
Supplier: Thermo Fisher Scientific
Description: Diaion® HP20 is used as a filler in chromatography columns due to its extremely large pores. It is considered as an effective adsorbent to eliminate toxic effects in fermentation and separation of traces heavy metal ions. It is also useful for the purification of peptides, proteins, fermentation products, polyphenols and cephalosporin C. It helps to remove bitter tasting substances from fruit juices.
UOM: 1 * 1 kg

MSDS Certificates


Product Image-786-707

Protein desalting and buffer exchange columns, SpinOUT™

Supplier: G-Biosciences
Description: The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin format columns for the desalting and buffer exchange of protein solutions ranging from 5 µl through to 4 ml sample volumes. The SpinOUT™ columns are available in three MWCO sizes for >700, >6000 or >30 000 dalton proteins and are suitable for samples containing as little as 20 µg protein/ml.

Certificates

Product Image-738-0108

Filter plates, 96-well, for DNA purification, AcroPrep™ Advance

Supplier: Cytiva (Formerly Pall Lab)
Description: The AcroPrep™ Advance filter plate portfolio includes a variety of membrane types and pore sizes to provide the best possible performance for a wide range of sample preparation and high throughput screening applications. AcroPrep™ Advance filter plates are ideal for concentration, purification and desalting of proteins and peptides; bead and chromatography resin-based applications; gross fractionation and lysate clarification, and general filtration.

Product Image-738-0113

Filter plates, 96-well, for protein purification, AcroPrep™ Advance

Supplier: Cytiva (Formerly Pall Lab)
Description: The AcroPrep™ Advance filter plate portfolio includes a variety of membrane types and pore sizes to provide the best possible performance for a wide range of sample preparation and high throughput screening applications. AcroPrep™ Advance filter plates are ideal for concentration, purification and desalting of proteins and peptides; bead and chromatography resin-based applications; gross fractionation and lysate clarification and general filtration.

Product Image-738-0129

Filter plates, 96-well, for lysate clearance, AcroPrep™ Advance

Supplier: Cytiva (Formerly Pall Lab)
Description: The AcroPrep™ Advance filter plate portfolio includes a variety of membrane types and pore sizes to provide the best possible performance for a wide range of sample preparation and high throughput screening applications. AcroPrep™ Advance filter plates are ideal for concentration, purification and desalting of proteins and peptides; bead and chromatography resin-based applications; gross fractionation and lysate clarification; and general filtration.

Product Image-PIER53132

Pierce™, UltraLink™ Affinity Chromatography Resin, Immobilised Protein A/G

Supplier: Thermo Fisher Scientific
Description: Pierce™ Protein A/G UltraLink™ and Protein A/G Plus UltraLink™ resins are extremely durable beaded polyacrylamide supports to which recombinant Protein A/G has been immobilised for use in IgG purification methods. Immoblised proteins are covalently coupled to an agarose support to provide an efficient method of purifying antibodies.

Product Image-PIER26150

Control agarose resin, Pierce™

Catalog Number: (PIER26150)
Supplier: Thermo Fisher Scientific
Description: Pierce™ Control Agarose Resin is underivatised beaded agarose support for pre-clearing protein immunoprecipitation samples.
UOM: 1 * 1 KIT
Product Image-AATB55016

ReadiPrep™ Protein G-Agarose Resin

Supplier: AAT BIOQUEST
Description: ReadiPrep™ Protein G-Agarose Resin is a high-quality affinity resin designed for the isolation and purification of immunoglobulin classes, subclasses, and fragments from mammalian serum and biological fluids.

New Product

Product Image-PIER44667

Pierce™ Protein Purification Kit, Protein A IgG Purification Kit

Catalog Number: (PIER44667)
Supplier: Thermo Fisher Scientific
Description: The Protein A IgG Purification Kit is useful for routine purification of antibodies. Samples containing IgG are incubated with immobilised Protein A in a buffer that facilitates binding. After non IgG components are washed from the column, the bound IgG is recovered by elution. The supplied buffers provide for maximum immunoglobulin binding and elution efficiency with the Protein A column.
UOM: 1 * 1 KIT
Product Image-PIER20505

Coupling Resin, AminoLink™ Plus

Supplier: Thermo Fisher Scientific
Description: AminoLink™ Plus Coupling Resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines to prepare columns for affinity purification.

Product Image-PIER53151

Immobilised NeutrAvidin™, UltraLink™, Pierce™

Supplier: Thermo Fisher Scientific
Description: NeutrAvidin™ UltraLink™ resin is a beaded polyacrylamide resin of immobilised NeutrAvidin™ Protein, a modified form of avidin with exceptional biotin-binding characteristics for affinity purification methods.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
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The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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