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Tris-Acetate-EDTA (TAE) buffer, 10X, molecular biology grade

Supplier: PanReac AppliChem
Description: 10X concentrated solution of 40 mM Tris, 20 mM glacial acetic acid and 1 mM EDTA.Na₂, pH 8,5 ±0,2.

Certificates

Product Image-E406-5ML

Ethidium bromide 0,625 mg/l in aqueous solution, Biotechnology Grade

Catalog Number: (E406-5ML)
Supplier: VWR Chemicals
Description: Ethidium bromide solution is a fluorescent stain for visualising nucleic acids in solution or in electrophoresis gels. The solution is provided at a concentration of 0,625 mg/ml. A final working concentration of 0,5 g/ml is easily obtained by adding one drop of solution to 50 ml of molten agarose, running buffer or staining buffer. Ethidium bromide solution in dropper bottles may be stored at room temperature for up to one year.
UOM: 1 * 5 mL

MSDS Certificates


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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck
Description: TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble, and protected from degradation. This concentrate can be easily diluted to 1X or 0,5X before use (with molecular biology grade water).

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CleanCap® AG (3′ OMe) CleanScript™ IVT Kit

Catalog Number: (SOLUK-7413-25)
Supplier: TriLink BioTechnologies
Description: <p><span style="color:black">TriLink’s CleanCap® AG (3′ OMe) CleanScript™ IVT kit provides essential components for mRNA synthesis using co-transcriptional capping by in vitro transcription (IVT). The kit components are sufficient to run 25 of 100 µl reactions following our recommended IVT protocol with co-transcriptional capping. It includes a cap analog, modified and unmodified nucleotides, IVT enzymes including CleanScribe RNA polymerase, 10X CleanScript IVT reaction buffer, and a linearized FLuc DNA control template.</span></p>
UOM: 1 * 100 µl

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Collagenase, MP Biomedicals

Catalog Number: (ICNA0215070401)
Supplier: MP Biomedicals
Description: For typical assay, dissolve enzyme at a concentration of 1 mg/ml in 0,05 M TES with 0,36 mM calcium chloride, pH 7,5. Dilutions run are 1/10 and 1/20 in the above buffer.
UOM: 1 * 1 g
Product Image-700-0292

VWR® shiroGEL Modular mini vertical PAGE systems

Catalog Number: (700-0292)
Supplier: VWR Collection
Description: The shiroGEL mini vertical PAGE system is modular, allowing PAGE and electroblotting to be carried out in the same tank, simply by changing the inserts. The system features inserts for each application, and a common buffer tank in which to run them. Each insert has its own electrode assembly, which connects to the lid of the buffer tank. For safety, the power leads connect directly to the lid. When the lid is removed, power is disconnected from the system. The inserts also have a small pad that fits into position in the lid. This ensures that the lid is always properly placed and aids in its removal. The buffer tank is moulded to prevent separation and leaking. A variety of spacers, combs and other accessories are available to customise the system. All spacers and combs are colour coded or clearly labelled to indicate thickness.
UOM: 1 * 1 items
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Fragment Analyzer Parts and Accessories

Supplier: AGILENT
Description: Search for genuine spare parts and accessories for the Fragment Analyzer systems to keep your operations running. Find out more here.

Product Image-730-2205

Horizontal electrophoresis system, RunOne™

Supplier: EMBITEC
Description: The ultra compact RunOne™ electrophoresis system is able to run a whole variety of gel sizes from mini to long in less than 350 ml of buffer. The power supply with selectable 25, 50 or 100 V output slides directly into the running unit. The system can run eight to 108 samples in minutes, with the separate casting system allowing up to six gels to be cast and run at the same time.

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Rehydration reagent, DeStreak

Supplier: Cytiva
Description: The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7,0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.

Product Image-786-123

Sample clean up, PAGE-Perfect™

Supplier: G-Biosciences
Description: Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis. The presence of contaminants, or interfering agents, such as salts, acids, bases and detergents, result in band distortion and poor protein resolution. As a result, SDS-PAGE gels are hard to analyse and lack reproducibility. PAGE-Perfect™ is a simple, two-step method for concentrating, cleaning and preparing protein solutions for running publication quality gels.

Product Image-730-0263

Dual-gel electrophoresis system, Owl™ P10DS

Catalog Number: (730-0263)
Supplier: THERMO OWL SCIENTIFIC
Description: The Owl™ P10DS dual gel system casts and runs one or two 20×20 cm gels in the upper buffer chamber. The larger size cassette allows the maximum number of samples and separation length. The system is ideal for second dimension protocols of 2-D electrophoresis applications.
UOM: 1 * 1 items
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Glycine ≥99% for molecular biology

Supplier: MP Biomedicals
Description: Storage: Store at Room Temperature (15-30 °C)
Glycine is a non-essential amino acid. It is only amino acid with no asymmetric carbon and thus is not chiral. It is the major inhibitory neurotransmitter. It is involved in the biosynthesis of the porphyrin rings of hemes and chlorophylls.
Glycine is commonly used in buffer solutions, in electrophoresis and preparative chromatography. A study of the folding of monoclonal antibodies in the presence of glycine and their subsequent purification has been published. The use of glycine in the purification of lipopolysaccharides, lipooligosaccharides, and lipid A has been reported. It is an amino acid for use in cell culture media development applications and existing media formulations. Glycine is commonly used as a component in Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis, a component of Towbin's transfer buffer for Western blots, a buffer substance in cryoenzymology, in osmotic pressure maintenance in isoelectric focusing of erythrocytes, salting-in effect in protein chemistry, and as a buffer component in the coupled phosphatase-kinase reaction for end labelling of restriction fragments. The growth requirements of various microorganisms is reported in the Handbook of Microbiology.
Glycine is a non-chiral amino acid that can be synthesized in the body from the amino acid serine by Serine Hydroxymethyltransferase. Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

Product Image-730-0183

Mini horizontal electrophoresis system, Owl™ B1A EasyCast™

Catalog Number: (730-0183)
Supplier: THERMO OWL SCIENTIFIC
Description: The Owl™ B1A EasyCast™ mini gel system is designed to provide flat, even banding patterns and consistent results with minimum effort. This system is ideal for determination of molecular weight ranges of PCR fragments or larger sized DNA molecules. The all-in-one design allows the option of casting and running a gel in the buffer chamber, eliminating the need for additional equipment.
UOM: 1 * 1 items
Product Image-730-0199

Mini horizontal gel electrophoresis systems, Owl™ B2 EasyCast™ and B3

Catalog Number: (730-0199)
Supplier: THERMO OWL SCIENTIFIC
Description: The Owl™ B2 EasyCast™ mini horizontal gel system provides flexibility in a small footprint and is available with or without buffer exchange ports. The Owl™ B3 with built-in recirculation system prevents formation of pH and ionic gradients for high resolution and uniform reproducible results. The Owl™ B3 is ideal for long runs, multiple sample sets or RNA gels. It also eliminates uneven migration, band distortion or disassociation of pH dependent glycoxylated RNA molecules that can result when ionic depletion occurs. As the recirculation system is built into the buffer chamber, no external pumps, tubing or stirring bars are required.
UOM: 1 * 1 items
Product Image-BE-406

Experimentation kits, protein electrophoresis

Catalog Number: (BE-406)
Supplier: G-Biosciences
Description: Using the Protein Electrophoresis kit, students will learn the principles of various types of electrophoresis, including denaturing and non-denaturing electrophoresis, and how this powerful technique is used to analyse proteins. The kit will introduce students to the different separation matrices currently in use and will understand their differing separation properties and their role in protein analysis. Students have an option of casting their own electrophoresis gels using polyacrylamide or using pre-cast commercially available gels. This kit is provided with all of the reagents, buffers and supplies needed for casting acrylamide gels, preparing protein samples, running electrophoresis, and staining the gels for visualisation of protein bands. Test protein samples and protein standards are also provided.
UOM: 1 * 1 KIT
Product Image-554-2273

Anion exchange HPLC columns, PRP-X100

Supplier: HAMILTON BONADUZ
Description: This anion column line is designed to meet the special requirements of anion analysis. These four columns cover all aspects of standard anion analysis, separation of all 8 common anions in one run. The unique design of the highly cross-linked porous-packing material, and the strong covalent bonded surface chemistry, ensure excellent durability and high separation efficiency. These columns are designed to be used with carbonate-bicarbonate buffer in any concentration or with hydroxide gradient, and many other mobile phases.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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