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Anti-TP53 Mouse Monoclonal Antibody (CF488A) [clone: BP53-12]

Supplier: Biotium
Description: This MAb reacts with an N-terminal epitope (aa 16-25) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia.

Image Unavailable-BNC040013-100

Anti-TP53 Mouse Monoclonal Antibody (CF405S) [clone: BP53-12]

Supplier: Biotium
Description: This MAb reacts with an N-terminal epitope (aa 16-25) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia.

Product Image-K872-500ML

VWR® Transfer Buffer Concentrate, CAPS 10X

Catalog Number: (K872-500ML)
Supplier: VWR Chemicals
Description: For transfer of protein to membranes for sequencing or blotting.
UOM: 1 * 500 mL

MSDS


Product Image-K813-500ML

VWR® UltraPure, Buffer, PBS solution 10X concentrate (phosphate buffered saline), USP

Catalog Number: (K813-500ML)
Supplier: VWR Chemicals
Description: Cell culture buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)
UOM: 1 * 500 mL

MSDS Certificates


Image Unavailable-BNC040338-100

Anti-TP53 Rabbit Monoclonal Antibody (CF405S) [clone: PAb122]

Supplier: Biotium
Description: PAb122 binds to the C-terminus (aa370-378) of both wild type and mutated p53. When microinjected into nuclei, PAb122 blocked re-entry into the S-phase of the cell cycle. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. p53 Binds to a DNA consensus sequence, the p53 response element, and it regulates normal cell growth cycle events by activating transcription of genes, involved either in progression through the cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. p53 Is the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation.

Product Image-0804-4L

VWR®, SSC buffer, 20X, ultra-pure grade

Catalog Number: (0804-4L)
Supplier: VWR Chemicals
Description: SSC buffer is a hybridisation buffer for Northern and Southern blots.
UOM: 1 * 4 L

MSDS Certificates


Product Image-E703-1L

VWR® UltraPure, PBS and TBS wash buffers

Supplier: VWR Chemicals
Description: Wash buffers ideal for use in applications such as Western blotting and ELISA. Prevents high background by effectively removing unbound reagents.

MSDS Certificates

Image Unavailable-913-50ML

Trizmal™ Buffer, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY
Description: For use in the Naphthol AS-D Chloroacetate Esterase Kit No. 91-C.

Image Unavailable-HUPF0112054

Enclosed General Purpose Trolley

Catalog Number: (HUPF0112054)
Supplier: HUPFER METALLWERKE
Description: Trolley in robust, self-supporting and hygienic design, made of high-quality stainless steel. Ø 25 mm round tube frame with two welded, 1000 ×600 mm deep-drawn shelves with 10 mm high profile edge, lined with sound insulation on the underside, hygienically folded by 35 mm and rebated inwards, designed for easy cleaning. Push bars integrated on both sides into tube frame ensure good manoeuvrability. Trolley with stainless steel panels on three sides and double-wing doors with grip opening and magnetic catch. Four polypropylene disc bumpers serve as bumpers and protect trolley on all sides as well as building-side walls from being damaged. Trolley runs on four swivel casters of which two with total locks, Ø 125 mm, chrome-galvanised housing, plastic caster wheel with roller bearing, failsafe running tread identical to pneumatic casters, with pin fastening.
UOM: 1 * 1 items
Product Image-N552-500ML

VWR® Gentle ReView™, Stripping Buffer

Supplier: VWR Chemicals
Description: Gentle ReView™ Stripping Buffer is a mild, yet effective method for stripping primary and secondary antibodies from PVDF or nitrocellulose membranes.

Image Unavailable-1805-1VL

Phosphate Buffer Solution

Catalog Number: (1805-1VL)
Supplier: SIGMA ALDRICH MICROSCOPY
Description: Contains sodium and potassium phosphates. Intended for use in the Sigma Butyrate Esterase Procedure, No. 181B.
UOM: 1 * 1 items
Product Image-28-9180-08

Columns, PD MidiTrap™ G-25

Catalog Number: (28-9180-08)
Supplier: Cytiva
Description: PD MidiTrap G-25 is convenient and rapid cleanup with high reproducibility and the potential for high throughput by running multiple samples in parallel, The use of centrifugation protocol with all gravity columns.
UOM: 1 * 50 items

MSDS


Product Image-17-5498-15

Affinity chromatography columns, HiScreen™ MabSelect™ PrismA

Catalog Number: (17-5498-15)
Supplier: Cytiva
Description: These columns are prepacked with MabSelect™ PrismA protein A chromatographyn resin that has been improved with an optimised high-flow agarose base matrix and a genetically engineered protein A-derived ligand, allowing efficient cleaning between purification runs. These columns are suitable for purifying antibodies with affinity chromatography in research and process development.
UOM: 1 * 1 mL

MSDS


Product Image-730-0188

Mini horizontal electrophoresis systems, Owl™ B1 EasyCast™

Catalog Number: (730-0188)
Supplier: THERMO OWL SCIENTIFIC
Description: The durable, easy‑to‑use, Owl™ B1 EasyCast™ mini horizontal gel system is suitable for running between five and 28 samples and is ideal for most teaching applications.
UOM: 1 * 1 items
Product Image-131-0018

Chemistry diaphragm pumps, VARIO® select

Supplier: VACUUBRAND
Description: The VACUU·SELECT controller simplifies your work and allows VARIO select chemistry diaphragm pumps to deliver unparalleled precision. The pumps run whisper quiet and respond to demand in real time by matching pumping speed to process conditions. The intuitive VACUU·SELECT controller includes predefined applications for all common vacuum processes.

Product Image-PRSI50-255

Anti-NEFL Mouse Monoclonal Antibody

Catalog Number: (PRSI50-255)
Supplier: ProSci Inc.
Description: Neurofilaments are the 10nm or intermediate filament proteins specifically in neurons, and are composed predominantly of three major proteins called NF-
NF-M and NF-H. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68 kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease.
UOM: 1 * 100 µl
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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