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Supplier: Biotium
Description: PAb122 binds to the C-terminus (aa370-378) of both wild type and mutated p53. When microinjected into nuclei, PAb122 blocked re-entry into the S-phase of the cell cycle. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. p53 Binds to a DNA consensus sequence, the p53 response element, and it regulates normal cell growth cycle events by activating transcription of genes, involved either in progression through the cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. p53 Is the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation.

Supplier: VWR Chemicals
Description: HEPES buffer solution pH 7.3 (1 mol/l), VWR®
Catalog Number: (732-3879)
Supplier: Genscript
Description: eZwest™ Lite is an automated western blotting device providing an easy and simple solution in your western blotting workflow. After proteins are transferred onto the membrane, load the membrane into our eZwest™ Lite device for automated blocking, incubation, and washing cycles. The user only needs to prepare the required reagents, run the customised program, and eZwest™ Lite will take care of the rest. Once the program is finished, the user can collect the results and immediately proceed to detection and analysis steps. eZwest™ Lite is easy to use and has minimal footprint.
UOM: 1 * 1 SET


Catalog Number: (115-0296)
Supplier: Texwipe
Description: The sealed-border technology used for these wipes produces four fully sealed borders to prevent the release of fibres and particles. The fabric is a no-run interlock, double knit from 100% continuous-filament polyester.
UOM: 1 * 1.000 items


Catalog Number: (444037E)
Supplier: VWR Chemicals
Description: Irradiated solution, pH 7,2, for washing blots.
UOM: 1 * 10 L

Catalog Number: (E521-100ML)
Supplier: VWR Chemicals
Description: With diethyl pyrocarbonate (DEPC).
UOM: 1 * 100 mL

Catalog Number: (M197-500ML)
Supplier: VWR Chemicals
Description: 4X concentrated buffer solution (1,5 M Tris, pH 8,8 and 0,4% SDS). Used for preparation of standard acrylamide:bis-acrylamide gels as well as preparation of gels using PAGE-PLUS™ acrylamide solution.
UOM: 1 * 500 mL


Catalog Number: (E691-500ML)
Supplier: VWR Chemicals
Description: TRIS buffer solution pH 7,5 (1 mol/l), VWR®
UOM: 1 * 500 mL

Catalog Number: (E1161-1000ML)
Supplier: SIGMA ALDRICH MICROSCOPY
Description: Used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
UOM: 1 * 1.000 mL


Supplier: Biotium
Description: PAb122 binds to the C-terminus (aa370-378) of both wild type and mutated p53. When microinjected into nuclei, PAb122 blocked re-entry into the S-phase of the cell cycle. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. p53 Binds to a DNA consensus sequence, the p53 response element, and it regulates normal cell growth cycle events by activating transcription of genes, involved either in progression through the cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. p53 Is the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation.

Catalog Number: (E522-100ML)
Supplier: VWR Chemicals
Description: Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l) for biotechnology
UOM: 1 * 100 mL

Supplier: Welch by Gardner Denver
Description: The MPC chemical duty diaphragm pumps use PTFE and other fluorinated plastics for the wetted parts to allow aggressive solvent and acid vapours to be pumped. If the pump should handle harsh chemical vapours, then the superior chemical resistant X2 version with higher grade seals and thicker coatings are the best choice. These are well known for quiet operation and running smoothness.

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Catalog Number: (E694-250ML)
Supplier: VWR Chemicals
Description: Cloning Buffer, TRIS buffer solution pH 9 (1 mol/l)
UOM: 1 * 250 mL

Supplier: VWR Chemicals
Description: Ready to use Tris-EDTA buffer.
Catalog Number: (PRSI48-862)
Supplier: ProSci Inc.
Description: Hsp27 ERE-TATA binding protein (HET)/ Scaffold Attachment Factor-B (SAF-B) is a member of a subset of nuclear matrix proteins that mediate the attachment of chromatin to nuclear protein complexes. The calculated molecular weight of HET/SAF-B is 102 kDa (Accession No. U72355), but on SDS PAGE gels HET/SAF-B runs as an approximately 130 kDa protein. This aberrant migration is due to a large number of charged amino acid residues.
UOM: 1 * 50 µG


Supplier: Biotium
Description: PAb122 binds to the C-terminus (aa370-378) of both wild type and mutated p53. When microinjected into nuclei, PAb122 blocked re-entry into the S-phase of the cell cycle. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. p53 Binds to a DNA consensus sequence, the p53 response element, and it regulates normal cell growth cycle events by activating transcription of genes, involved either in progression through the cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. p53 Is the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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