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You Searched For: X-Gal (5-Bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)


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Product Image-EDVO303

Experimentation kits, exploring biotechnology with green fluorescent protein (GFP)

Catalog Number: (EDVO303)
Supplier: EDVOTEK
Description: Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP). Bacterial cells are transformed to express the GFP. The transformed cells are then grown and the GFP purified by column chromatography. Finally, the purity of the protein fractions are analysed by SDS polyacrylamide electrophoresis.
UOM: 1 * 1 KIT
Product Image-EDVO255

Purification and size determination of green and blue fluorescent proteins

Catalog Number: (EDVO255)
Supplier: EDVOTEK
Description: When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP are used as an assay during their purification from an <i>E. coli</i> extract. The column fractions containing GFP or BFP are identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.
UOM: 1 * 1 KIT
Product Image-EDVO221

Transformation of E. coli with pGAL™

Catalog Number: (EDVO221)
Supplier: EDVOTEK
Description: In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the <i>lacZ</i> gene. IPTG is not required in this experiment since pGAL contains the complete <i>lacZ</i> gene.
UOM: 1 * 1 items
Product Image-EDVO124

Kits: DNA-based screening for smallpox, Ready-to-Load™

Catalog Number: (EDVO124)
Supplier: EDVOTEK
Description: This experiment presents a bioterrorism scenario, with students examining a simulated DNA fingerprinting test for the detection of smallpox.
UOM: 1 * 1 items
Product Image-EDVO651

Precast polyacrylamide gels

Supplier: EDVOTEK
Description: Precast gel, 6 gels (12%)

Image Unavailable-EDVO630

Microtest tubes

Catalog Number: (EDVO630)
Supplier: EDVOTEK
Description: 500×1,5 ml tubes.
UOM: 1 * 500 items
Product Image-612-3731

Pipette stand for six micropipettes

Catalog Number: (612-3731)
Supplier: EDVOTEK
Description: Stand, pipette, For: 6 pipettes
UOM: 1 * 1 items
Product Image-EDVOS-44

Micropipetting basics

Catalog Number: (EDVOS-44)
Supplier: EDVOTEK
Description: Kit for teaching students how to use a micropipette with ease and accuracy using multi-coloured dyes.
UOM: 1 * 1 items
Product Image-EDVO110

Experimentation kits, molecular weight determination of proteins (agarose-based)

Catalog Number: (EDVO110)
Supplier: EDVOTEK
Description: This experiment introduces a simple method to determine protein subunit molecular weights using horizontal electrophoresis. As the protein standards and 'unknowns' are prestained, the separation of proteins can be observed during electrophoresis. Included in the experiment is protein-grade agarose, which provides an alternative to the use of polyacrylamide gels.
UOM: 1 * 1 KIT
Product Image-EDVO108

Experimentation kits, principles of gel filtration chromatography

Catalog Number: (EDVO108)
Supplier: EDVOTEK
Description: This experiment introduces chromatographic separation to the class and shows students how dyes of different colours separate on the basis of their size and shape. Columns may be rinsed and reused.
UOM: 1 * 1 items

MSDS


Product Image-EDVO130-C

Kits: amplification of DNA for fingerprinting

Supplier: EDVOTEK
Description: Ready-to-Load™ kit for teaching DNA fingerprinting and to show how DNA evidence is used in modern forensics. This experiment allows for varied results depending on the selection of DNA fingerprinting patterns.

Product Image-EDVO235

Experimentation kits, DNA/RNA microarrays

Catalog Number: (EDVO235)
Supplier: EDVOTEK
Description: Microarray technology allows scientists to screen large numbers of samples in one assay. This technology allows high-throughput screening of small samples and allows comparisons to be made between healthy and unhealthy tissues. In this experiment, students apply simulated DNA and RNA samples to a membrane to screen for positive and negative samples.
UOM: 1 * 1 KIT
Product Image-EDVO119

Genes in a Tube™

Catalog Number: (EDVO119)
Supplier: EDVOTEK
Description: Kit for teaching students how to extract and precipitate their own DNA. Students can transfer their DNA to a tube that can be used as a pendant on a necklace.
UOM: 1 * 1 items
Product Image-EDVO117-C

Kits: detection of mad cow disease, Ready-to-Load™

Supplier: EDVOTEK
Description: Bovine spongiform encephalophathy (BSE), also known as Mad Cow Disease, is a neurodegenerative, fatal condition in cattle. Consuming BSE-infected beef is believed to be the cause of a similar condition in humans, Creutzfeldt-Jakob disease. In this experiment, students examine simulated PCR products from several feed mills, to determine any possible violations of the ban on including animal parts in cattle feed.

Product Image-EDVO203

Experimentation kits, isolation of E. coli Chromosomal DNA

Catalog Number: (EDVO203)
Supplier: EDVOTEK
Description: Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA extraction LyphoCells and reagents for isolating chromosomal DNA from <i>E. coli</i>. After spooling from solution, the DNA can be dissolved and analysed by agarose gel electrophoresis as an optional lab extension activity.
UOM: 1 * 1 KIT
Product Image-EDVO315

Kits: in search of the sickle cell gene by southern blot, Ready-to-Load™

Catalog Number: (EDVO315)
Supplier: EDVOTEK
Description: Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane the method allows the precise analysis and identification of DNA bands on a gel. Using this kit, students use Southern blotting to find a point mutation in the haemoglobin gene indicating Sickle Cell Anaemia.
UOM: 1 * 1 items

MSDS


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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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