You Searched For: G Bioscienes

Catalog Number: (786-453)

Supplier: G-Biosciences

Description: The Phosphatase Assay Kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases. The Phosphatase Assay Kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases.

UOM: 1 * 1 KIT

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Catalog Number: (GENO786-1996)

Supplier: G-Biosciences

Description: T4 GP32 is a single-stranded DNA binding protein with key role in DNA replication, recombination and repair. T4 GP32 binds cooperatively to single stranded DNA and stimulates cell-free DNA synthesis directed by the T4-encoded DNA polymerase. It improves the efficiency of reverse transcriptase during RT-PCR as well as increases the yield of PCR products.

UOM: 1 * 500 µG

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Supplier: G-Biosciences

Description: GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G-Biosciences

Description: GET™ Plasmid DNA Miniprep Kit isolates high quality plasmid DNA from 1 to 5 ml<i> E. coli </i>cultures. The kit utilises an enhanced DNA binding column to produce high yields of plasmid.

Supplier: G-Biosciences

Description: The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions.

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Supplier: G-Biosciences

Description: The Micro Bicinchoninic Acid Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions. This modification of the Bicinchoninic Acid (BCA) Protein Assay is suitable for dilute protein samples (0,5 to 20 µg/ml).

Catalog Number: (786-501)

Supplier: G-Biosciences

Description: A protein solubilisation buffer for 2D analysis must solubilise proteins effectively, without disturbing the native charge of the proteins. Urea based solubilisation buffers solubilise proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results.

UOM: 1 * 50 mL

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Supplier: G-Biosciences

Description: These DNase- and RNase-free buffers and reagents are designed for high quality research.

Supplier: G-Biosciences

Description: These DNase- and RNase-free buffers and reagents are designed for high quality research.

MSDS

Catalog Number: (R041)

Supplier: G-Biosciences

Description: Ethidium bromide 0.625 mg/ml in aqueous solution

UOM: 1 * 5 mL

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Catalog Number: (BE-417)

Supplier: G-Biosciences

Description: The Affinity Chromatography kit teaches the basic principle of affinity chromatography utilising highly specific affinity columns. This lab activity involves preparation of a crude protein extract and running affinity exchange chromatography for isolation of a protein.

UOM: 1 * 1 KIT

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Catalog Number: (786-430)

Supplier: G-Biosciences

Description: SAM510™ assay is a continuous enzyme coupled assay that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.

UOM: 1 * 100 Assays

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Supplier: G-Biosciences

Description: FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494 nm and 520 nm, respectively, therefore emitting green visible light.

Supplier: G-Biosciences

Description: These CL (Compatible Lowry) Protein Assay is based on the widely cited protein assay by Lowry et. al. (1951). The CL Protein Assay improves upon the traditional Lowry method to be compatible with common laboratory agents known to interfere with Lowry protein assays such as reducing agents (dithiothreitol (DTT), ß-mercaptoethanol and TCEP), detergents, chelating agents, amines, sugars, strong chaotropic buffers, salts, drugs, antibiotics, cobalt and other common laboratory agents.

Supplier: G-Biosciences

Description: PAGE-Optimizer™ is a unique, spin column for the preparation of samples for SDS-PAGE. The columns contain a proprietary separation matrix that removes contaminants such as salts, detergents, cellular agents and other common buffering agents. PAGE-Optimizer™ helps eliminate smeared lanes, distorted and/ or smiling bands and swollen lanes. PAGE-Optimizer™ has also been shown to reveal protein bands previously masked by the above interfering agents.

Catalog Number: (GENO786-333)

Supplier: G-Biosciences

Description: A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Yeast/ Fungal ProteaseArrest™ inhibits yeast and fungal serine, cysteine and metalloproteases.

UOM: 1 * 1 mL

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