You Searched For: Thermo Fisher Scientific

Catalog Number: (PIER23011)

Supplier: Thermo Fisher Scientific

Description: Pierce™ MMTS (methyl methanethiosulfonate) is a small compound that reversibly blocks cysteines and other sulfhydryl groups, enabling the study of enzyme activation and other protein functions.

UOM: 1 * 200 mg

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Catalog Number: (PIER22339)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce SBAP is a short (6.2 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and bromoacetyl reactive groups.

UOM: 1 * 50 mg

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Catalog Number: (PIER28300)

Supplier: Thermo Fisher Scientific

Description: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate (CHAPS)

UOM: 1 * 5 g

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Catalog Number: (PIER24110)

Supplier: Thermo Fisher Scientific

Description: Guanidinium hydrochloride

UOM: 1 * 500 g

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Supplier: Thermo Fisher Scientific

Description: Pierce™ Protease Assay Kits are an ideal choice for performing routine assays necessary during the isolation of proteases, or for identifying the presence of contaminating proteases in protein samples. These protease assays are also ideal for studying pH or temperature vs. activity profiles of purified proteases.

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Supplier: Thermo Fisher Scientific

Description: Pierce™ HRP conjugated avidin is used in biotin-based detection methods, including Western blotting or ELISA detection with appropriate enzyme substrates.

Catalog Number: (PIER37615)

Supplier: Thermo Fisher Scientific

Description: The 1-Step™ ABTS (2,2'-azinobis [3-ethylbenzthiazoline-6-sulfonic acid] diammonium salt) is a ready-to-use, water-soluble ELISA substrate for horseradish peroxidase. This substrate yields a green product with absorbance maxima at 410 nm and 650 nm. Adding SDS to the substrate stops the reaction without changing the colour.

UOM: 1 * 250 mL

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Catalog Number: (PIER32400)

Supplier: Thermo Fisher Scientific

Description: Pierce™ Recombinant Peroxidase Conjugated Protein A can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

UOM: 1 * 1 mg

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Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.

Catalog Number: (PIER21578)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.

UOM: 1 * 50 mg

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Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce TCEP-HCl is a potent, versatile, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds. The unique compound is easily soluble and very stable in many aqueous solutions. TCEP reduces disulfide bonds as effectively as dithiothreitol (DTT), but unlike DTT and other thiol-containing reducing agents, TCEP does not have to be removed before certain sulfhydryl-reactive crosslinking reactions.

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Supplier: Thermo Fisher Scientific

Description: Imject™ BSA maleimide-activated enables conjugation of sulfhydryl-containing peptide antigens to immunogenic bovine serum albumin carrier protein for immunisation and antibody production.

Supplier: Thermo Fisher Scientific

Description: These Ni(2+) chelate-coated plates are ideal for analysing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking. The clear, white or black plates can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.

Supplier: Thermo Fisher Scientific

Description: Nitrocellulose membranes are a popular matrix used in protein blotting because of their high protein-binding affinity, compatibility with a variety of detection methods (chemiluminescence, chromogenic, and fluorescence), and the ability to immobilise proteins, glycoproteins, or nucleic acids. Protein immobilisation is thought to occur by hydrophobic interactions, and high salt and low methanol concentrations help improve protein immobilisation to the membrane during electrophoretic transfer, especially for proteins with higher molecular weights. Nitrocellulose membranes are not optimal for electrophoretic transfer of nucleic acids, as the high salt concentrations that are required for efficient binding will effectively elute some or all of the charged nucleic acid fragments.

Catalog Number: (PIER77110)

Supplier: Thermo Fisher Scientific

Description: Imject™ BSA in phosphate buffered saline (PBS) is purified bovine serum albumin carrier protein that enables simple preparation of effective immunogens with amine- or carboxyl-peptide antigens.

UOM: 1 * 1 SET

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Catalog Number: (PIER20307)

Supplier: Thermo Fisher Scientific

Description: Pierce™ Immobilised p-Aminophenyl Phosphoryl Choline Gel enables purification of C-reactive protein (CRP) from plasma, ascites and other biological fluids.

UOM: 1 * 5 mL

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