You Searched For: STEMCELL Technologies

Supplier: STEMCELL Technologies

Description: Immunomagnetic positive selection of CD11b+ cells from human peripheral blood mononuclear cells (PBMCs) or leukapheresis samples.

Supplier: STEMCELL Technologies

Description: R-Spondin-1 (RSPO1) is the prototype member of the R-Spondin (RSPO) protein subfamily of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins (Chen <i>et al.</i>; Kamata <i>et al.</i>; Kazanskaya <i>et al.</i>; Kim <i>et al.</i>). Although unable to initialize signaling, RSPO family members are potent enhancers of WNT signaling (Cruciat and Niehrs; de Lau <i>et al.</i>; Kamata <i>et al.</i>; Kazanskaya <i>et al.</i>). They are characterized by a TSR-1 domain, a carboxy-terminal region with positively charged amino acids, and two N-terminal furin-like cysteine-rich repeats (Glinka <i>et al.</i>; Kazanskaya <i>et al.</i>). R­-Spondin-1 activates β­-catenin signaling via the WNT signaling cascade and by indirectly increasing low-density lipoprotein receptor-related protein 6 (LRP6) on the cell surface. It does this by binding leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5), and competing with WNT antagonist DKK­1 for binding to the WNT co­receptors, Kremen and LRP­6, which reduces DKK­1-­mediated internalization of LRP6 (Binnerts <i>et al.</i>). RSPO1 is involved in a wide range of pleiotropic roles during embryogenesis, it is required for the specification of hematopoietic stem cells, and it has been shown to be important in the growth, survival, and migration of ovarian cancer cells (Cruciat and Niehrs; de Lau <i>et al.</i>; Genthe and Clements; Liu <i>et al.</i>).

Supplier: STEMCELL Technologies

Description: Bone morphogenetic protein 4 (BMP-4) is a member of the highly conserved transforming growth factor β (TGF-β) superfamily. Mature BMP-4 is a disulfide-linked homodimeric protein consisting of two 116-amino-acid residue subunits, and is generated by the proteolytic removal of the signal peptide and propeptides (Xiao <i>et al.</i>). BMP-4 binds to type I and type II receptors on cells. This binding results in the phosphorylation of receptor 1, which in turn results in the phosphorylation of Smad proteins, which then go on to act as transcription factors (Zhang <i>et al.</i>). BMPs have been shown to be key regulators of embryogenesis and are known to play a role in the growth and differentiation of various cell types, including embryonic stem (ES) cells, induced pluripotent stem (iPS) cells, mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells (Chadwick <i>et al.</i>; Graham <i>et al.</i>; Jones <i>et al.</i>; Lengerke <i>et al.</i>; Zhang <i>et al.</i>).

Supplier: STEMCELL Technologies

Description: Receptor activator of nuclear factor kappa-B ligand (RANKL) is a member of the tumor necrosis factor (TNF) superfamily (Anderson <i>et al.</i>). Cytokines in the TNF superfamily are involved in a variety of long-term cellular activities such as differentiation, proliferation, and cell death (MacEwan). RANKL is a type II homotrimeric transmembrane protein expressed in both a membrane-bound and secreted form (Ikeda <i>et al.</i>). RANKL binds to the receptor activator of nuclear factor kappa-B (RANK). Upon binding to its receptor, RANKL activates the AKT signaling pathway (Moon <i>et al.</i>). Osteoprotegerin (OPG) may also bind RANKL, and this binding competes with RANKL-RANK binding (Lacey <i>et al.</i>). RANKL is involved in osteoclastogenesis (Lacey <i>et al.</i>; Yasuda <i>et al.</i>) and T cell activation (Wong <i>et al.</i>).

Catalog Number: (STMC100-0651)

Supplier: STEMCELL Technologies

Description: Media and supplements for differentiation of human pluripotent stem cells into blood vessels organoids.

UOM: 1 * 1 KIT

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Catalog Number: (STMC60135FI.1)

Supplier: STEMCELL Technologies

Description: Mouse monoclonal IgG2b antibody against human, rhesus, cynomolgus CD32, FITC-conjugated.

UOM: 1 * 100 Tests

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Supplier: STEMCELL Technologies

Description: Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD2, FITC-conjugated.

Supplier: STEMCELL Technologies

Description: Rat (WI) monoclonal IgG2b antibody against mouse TER119.

Supplier: STEMCELL Technologies

Description: Fibroblast growth factor 16 (FGF-16) is a heparin-binding member of the FGF family. FGFs possess broad mitogenic and cell survival activities and are expressed during embryonic development. FGFs act primarily on cells of mesodermal and neuroectodermal origin to regulate diverse physiological functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, and tissue repair (Goldfarb; Green <i>et al.</i>). In vitro, FGF-16 has been shown to promote the proliferation of brown adipocytes and in rat embryos it is predominantly expressed in these cells. FGF-16 has also been shown to play a critical role in embryonic heart development and is thought to play a cardioprotective role after birth (Hotta <i>et al.</i>; Lu <i>et al.</i>; Wang <i>et al.</i>).

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Catalog Number: (STMC08630)

Supplier: STEMCELL Technologies

Description: Cell culture medium kit for robust generation of ventral forebrain neural organoids from human pluripotent stem cells.

UOM: 1 * 1 KIT

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Catalog Number: (STMC100-0676)

Supplier: STEMCELL Technologies

Description: The SARS-CoV-2 (spike protein) peptide pool is provided as two lyophilised mixtures (subpools) from the SARS-CoV-2 spike protein. Each subpool contains 158 peptides, for a total of 316 peptides. The virus attaches to the cell membrane of the host through the interaction between spike protein and angiotensin-converting enzyme 2 (ACE2) receptor, and the spike protein plays a critical role in viral entry. The subpools consist of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 1273 on the spike protein.

UOM: 1 * 2 items

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Catalog Number: (STMC100-0297)

Supplier: STEMCELL Technologies

Description: Rat monoclonal IgG2b, antibody against mouse MHC Class II (I-A/I-E), unconjugated.

UOM: 1 * 100 µG

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Catalog Number: (STMC100-0138)

Supplier: STEMCELL Technologies

Description: Mouse monoclonal antibody against human, rhesus, cynomolgus CD9, unconjugated.

UOM: 1 * 100 µG

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Supplier: STEMCELL Technologies

Description: Mouse monoclonal IgG1, antibody against human, chimpanzee CD4, APC-conjugated.

Supplier: STEMCELL Technologies

Description: SARS-CoV-2 Recombinant Spike Protein, aa16-685 is expressed in HEK293 cells and is one of four structural proteins encoded by the SARS-CoV-2 genome. The Spike Protein plays a key role in attachment to host cells, allowing invasion through clathrin-mediated endocytosis. The Spike Protein can be cleaved by host cell proteases after aa685 to yield the N-terminal S1 subunit and C-terminal S2 region. The S1 subunit is responsible for interacting with the host cell receptor (angiotensin-converting enzyme II) through a receptor-binding domain that is highly conserved with SARS-CoV. The S1 subunit has two conformations: a ‘down’ conformation in which the receptor is inaccessible, and an ‘up’ conformation in which the receptor is accessible. These conformational changes are key for monoclonal antibody drugs and vaccine development. SARS-CoV-2 Recombinant Spike Protein contains a polyhistidine tag at the amino terminus; it also contains a FLAG tag at the carboxy terminus.

Catalog Number: (STMC100-0386)

Supplier: STEMCELL Technologies

Description: Culture medium kit for initiation, growth, and differentiation of human liver organoids.

UOM: 1 * 1 KIT

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