You Searched For: Protein Expression

Description: Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins. Thiophilic adsorption was first developed by Porath et al in 1984 and is a group specific, salt-dependent purification technique that has distinct affinity towards immunoglobulins and α2- macroglobulins. The thiophilic adsorption works on the principle that some proteins in high salt are able to bind to an immobilised ligand that contains a sulfone group in proximity to a thioether group. The bound proteins are then eluted in decreasing salt concentrations. The thiophilic resin binds immunoglobulins, including IgG, IgY and IgM, from serum, ascites or tissue culture supernatants and the purified immunoglobulins are then eluted in a near neutral aqueous buffer.

Catalog Number: 786-267

UOM: 1 * 10 mL

Supplier: G-Biosciences

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Description: Proteasome Purification Kit is a high integrity kit for the isolation and purification of proteasome complexes from biological samples such as cell lysates, tissue extracts, plasma, serum, confirmed by Western blot analysis.

Catalog Number: ENZOBMLPW1075A0001

UOM: 1 * 10 Tests

Supplier: ENZO LIFE SCIENCES

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Description: Immobilised Protein A and G are designed for binding the constant domains of immunoglobulin (Ig) molecules. Immobilised Protein A is coupled to 6% highly cross-linked agarose beads by a proprietary coupling method that provides high coupling efficiency for immunoglobulins and minimal protein A leaching. The binding capacity of Protein A is 18 to 43 mg Human IgG per ml resin.

Catalog Number: GENO786-833

UOM: 1 * 5 mL

Supplier: G-Biosciences

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Description: For the rapid purification of soluble, 6X His or GST tagged proteins.

Catalog Number: 786-635

UOM: 1 * 1 KIT

Supplier: G-Biosciences

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Description: STRATEGY™ is designed to allow researchers to rapidly screen a number of purification techniques in a small scale format to identify the system to isolate their protein of interest. The self-contained kit allows researchers to save time and money as separate buffers, resins and columns are not needed.

Catalog Number: 786-184C

UOM: 1 * 6 items

Supplier: G-Biosciences

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Description: The affinity removal spin cartridge reagent kit is designed to remove the most interfering high-abundant protein (albumin) from human samples (such as plasma, serum, urine or cerebrospinal fluid).

Catalog Number: AGLS5188-5251

UOM: 1 * 100 items

Supplier: AGILENT

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Description: The Amicon® Pro purification system - A centrifugal tool collapsing protein purifcation with concentration and buffer exchange.

Catalog Number: MILFACS500024

UOM: 1 * 1 items

Supplier: Merck

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Description: The Pearl™ Monoclonal IgG Purification Kit allows for the rapid purification of antibodies from cell culture supernatant and ascites fluid. The Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The IgG molecules can be stored or used in downstream applications without further clean-up, such as ammonium sulphate precipitation.

Catalog Number: 786-802

UOM: 1 * 1 KIT

Supplier: G-Biosciences

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Description: The Pearl™ Antibody Clean Up Kit is designed for the rapid clean up of antibody solutions using a combination of our Pearl™ IgG Purification Resin to remove the protein stabilisers and SpinOUT™ desalting columns to ensure the antibody solutions are in an optimal buffer for clean up. It binds high abundant, non IgG proteins (i.e. BSA and gelatin) and allows the IgG molecules to pass through in a physiological buffer. For the purification of ten 0,5 ml IgG samples with up to 1% BSA and gelatin.

Catalog Number: 786-803

UOM: 1 * 1 KIT

Supplier: G-Biosciences

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Description: Starter reagent kit for multiple affinity removal LC columns.

Catalog Number: AGLS5185-5991

UOM: 1 * 25 items

Supplier: AGILENT

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Description: The Pearl™ IgG purification kits are designed for the one step purification of IgG antibodies from serum. The supplied Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The purified IgG molecules can be stored or used in further downstream applications without further clean-up, such as ammonium sulphate precipitation.

Catalog Number: 786-798

UOM: 1 * 1 KIT

Supplier: G-Biosciences

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Description: The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin format columns for the desalting and buffer exchange of protein solutions ranging from 5 µl through to 4 ml sample volumes. The SpinOUT™ columns are available in three MWCO sizes for >700, >6000 or >30 000 dalton proteins and are suitable for samples containing as little as 20 µg protein/ml.

Catalog Number: 786-705

UOM: 1 * 5 items

Supplier: G-Biosciences

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Description: AminoLink™ Plus immobilisation kit contains AminoLink™ Plus coupling resin and additional reagents to perform five protein immobilisations, with expected yields of 2 to 40 mg protein per column. The coupling resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines to prepare columns for affinity purification.

Catalog Number: PIER44894

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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Description: The Pierce™ Crosslink Magnetic IP/Co-IP Kit uses crosslinking chemistry to covalently immobilise IP antibodies onto premium-quality protein A/G magnetic beads for effective immunoprecipitation and co-immunoprecipitation.

Catalog Number: PIER88805

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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Description: Pierce™ c-Myc Tag IP/Co-IP Kit provides a simple and fast method to study c-Myc-tagged proteins, with advantages over the traditional IP procedure using protein A/G agarose.

Catalog Number: PIER23620

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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Description: Pierce™ Protein IgG Plus Orientation Kits uses a crosslinker to covalently attach antibody that is bound to Protein A (or Protein G) agarose resin, thereby creating a reusable affinity column for purification of antigens.

Catalog Number: PIER44990

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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