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You Searched For: Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.


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Reactive oxygen species indicators dyes

Supplier: Biotium
Description: Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. It is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. It is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) and peroxynitrite. Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 but with increased stability toward air oxidation and light during storage. Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (355/420 nm) in cells, while the oxidized form ethidium has red fluorescence (518/605 nm) upon DNA intercalation. H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein, 504/529 nm (end product).

MSDS

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Biotin kits, Micro HOOK™

Supplier: G-Biosciences
Description: The micro HOOK™ Biotin kits are designed to label small amounts of proteins, with each kit designed for 8 to 10 labellings of 50 to 250 µg protein/reaction.

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Biotin SE protein labelling kit

Catalog Number: (92224.)
Supplier: Biotium
Description: This kit contains everything needed to label antibodies or other proteins with biotin succinimidyl ester (biotin SE). Each kit contains reagents for three labelling reactions of up to 1 mg protein per reaction.
UOM: 1 * 1 KIT
Product Image-COBSD4-000

B-Phycoerythrin (BPE)

Catalog Number: (COBSD4-000)
Supplier: Columbia Biosciences
Description: Because of its high quantum yield, B-PE is considered the world’s brightest fluorophore.
UOM: 1 * 1 mg

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Product Image-29028-1

CF™ Dye lectin conjugates

Supplier: Biotium
Description: A lectin labelled with our superior fluorescent CF® dyes is an effective and widely used cell surface stain for yeast, fungi, and mammalian cells.

Product Image-786-404

CDI amine reactive resin

Catalog Number: (786-404)
Supplier: G-Biosciences
Description: CDI amine reactive agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates.
UOM: 1 * 10 mL
Product Image-29047.

Annexin V Near IR CF® dye conjugates

Supplier: Biotium
Description: Near-IR Annexin V fluorescent conjugates can be used to detect apoptotic cells and is suitable for <i>in vivo</i> imaging.

Product Image-786-728

IgG Biotinylation kits, HOOK™

Supplier: G-Biosciences
Description: HOOK™ IgG biotinylation kits are designed for the biotinylation of IgG molecules by immobilising the IgG molecules on a solid support. Nickel chelating resin allows for the rapid removal of uncoupled biotin and therefore eliminates the need for further dialysis or desalting of the biotinylated antibody.

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Lipophilic carbocyanine dyes and CellBrite cytoplasmic membrane labelling kits

Supplier: Biotium
Description: Carbocyanine dyes with long hydrocarbon chains have low fluorescence in aqueous phase, but become highly fluorescent upon binding to lipid bilayers, making this class of dyes ideal for staining cell membranes. Carbocyanine dye membrane staining is very stable, with minimal dye transfer between cells. Dilinoleyl carbocynaine dyes have much faster lateral diffusion rates than regular carbocyanine dyes, which facilitates labelling of neurons in tissues.

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Biotin picolyl azide

Catalog Number: (92186.)
Supplier: Biotium
Description: Biotin picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency.
UOM: 1 * 0,5 mg
Product Image-92174.

Dye tyramide, CF®

Supplier: Biotium
Description: Fluorescent CF® dye tyramides are used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolour immunocytochemistry (ICC), immunohistochemistry (IHC), or <i>in situ</i> hybridization (ISH).

Image Unavailable-ENZOENZ51032K100

ORGANELLE-ID-RGB® III Assay kit

Catalog Number: (ENZOENZ51032K100)
Supplier: ENZO LIFE SCIENCES
Description: TheORGANELLE-ID-RGB® III assay kit contains Golgi apparatus-selective, endoplasmic reticulum-selective and nucleus-selective dyes suitable for live cell staining.
UOM: 1 * 1 KIT
Product Image-ENZOENZ42842L0050

Orange 552 dUTP (lyophilised)

Catalog Number: (ENZOENZ42842L0050)
Supplier: ENZO LIFE SCIENCES
Description: Orange 552 dUTP (lyophilised)
UOM: 1 * 50 Nmo
Image Unavailable-ENZOENZ42842

Orange 552 dUTP

Catalog Number: (ENZOENZ42842)
Supplier: ENZO LIFE SCIENCES
Description: Orange 552 dUTP (5-TAMRA-dUTP) can replace TTP in reactions in which it serves as a substrate for <i>E. coli</i> DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Orange 552 dUTP can also be used for multicolor fluorescence labeling.
UOM: 1 * 25 Nmo
Product Image-92266.

Antibody labelling kits, Mix-n-Stain™ Biotin

Supplier: Biotium
Description: Mix-n-Stain™ Biotin antibody labelling kits utilise a technology that allows rapid antibody labelling with biotin without a purification step.

MSDS

Product Image-29011.

Annexin V conjugates

Supplier: Biotium
Description: Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
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The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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