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You Searched For: Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.


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Phalloidin conjugates

Supplier: Biotium
Description: Phalloidin is a toxin isolated from the deadly <i>Amanita phalloides</i> mushroom. It is a bicyclic peptide that binds specifically to F-actin. Fluorescent conjugates of phalloidin are convenient tools to investigate the distribution of F-actin in fixed and permeabilised cells or tissue sections. One unit (U) of phallodin is sufficient to stain one sample (200 µl staining volume). A highly selective actin filament stain for fixed and permeabilised cells. Labeled phalloidins have similar affinity for both large and small filaments.

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Stabiliser for sensitive biomolecules, SeramunStab® STB

Supplier: Apollo Scientific
Description: Liquid product colourless to slightly yellow, freshly solutions opaleszent, odour weakly pH 7,3 to 7,5 boiling point 104 °C. Stabiliser for sensitive biomolecules like proteohormones or membrane proteins.

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Stabiliser for calibrators and controls, SeramunStab® STD

Catalog Number: (APOSBIL3120-100ML)
Supplier: Apollo Scientific
Description: Liquid product yellow odourless pH-value 7,3 to 7,5 Boiling point 102 °C. Stabiliser for complex mixtures of biomolecules.
UOM: 1 * 100 mL
Product Image-786-809

Carboxyl coupling resin

Supplier: G-Biosciences
Description: Carboxyl coupling resin consists of 6% cross-linked agarose with covalently linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. Molecules, including proteins, peptides and nucleic acids, are covalently coupled to the free primary amines, and the stable columns are ideal for affinity purification of antibodies and other interacting partners.

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Phosphothreonine detection set

Catalog Number: (ENZOALX850024KI01)
Supplier: ENZO LIFE SCIENCES
Description: This kit offers a set of three different monoclonal antibodies specific for threonine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: Phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site.
UOM: 1 * 1 SET
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Phosphotyrosine detection set

Catalog Number: (ENZOALX850025KI01)
Supplier: ENZO LIFE SCIENCES
Description: This kit offers a set of seven different MAbs specific for tyrosine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: Phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Specificity has been determined by epitope mapping using degenerated phosphopeptide libraries.
UOM: 1 * 1 SET
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NEDDylation kit

Catalog Number: (ENZOBMLUW05900001)
Supplier: ENZO LIFE SCIENCES
Description: This kit provides the means of generating a thioester-linked NEDD8-conjugated E2 enzyme, utilizing the first two steps in the NEDD8 cascade, for use in the NEDDylation of E3 ligases and target substrate proteins (user supplied). A NEDD8-specific antibody is provided for detection of NEDDylated proteins via SDS-PAGE and Western blotting.
UOM: 1 * 20 Tests
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CGH labeling kit, CYTAG®

Supplier: ENZO LIFE SCIENCES
Description: Comparative genomic hybridisation (CGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. CGH provides an understanding of genetic disorders, cancers and other genomic aberrations.

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Red 650 dUTP

Catalog Number: (ENZOENZ42522)
Supplier: ENZO LIFE SCIENCES
Description: Red 650 [Cyanine-5E] dUTP is a cyanine-5 derivative with enhanced spectral properties. This nucleotide analog can replace TTP in reactions in which it serves as a substrate for <i>E.coli</i> DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labelling and 3'-end labelling.
UOM: 1 * 25 Nmo
Product Image-ENZOENZ42819

Bio-7-dATP

Catalog Number: (ENZOENZ42819)
Supplier: ENZO LIFE SCIENCES
Description: Bio-7-dATP (Biotin-7-2’-deoxyadenosine-5’-triphosphate) can replace dATP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.
UOM: 1 * 50 Nmo
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Fluorescein-12-UTP

Catalog Number: (ENZOENZ42834)
Supplier: ENZO LIFE SCIENCES
Description: Fluorescein-12-UTP is ideal for <i>in vitro</i> transcription reactions catalysed by T3, T7, or SP6 RNA polymerases and reverse transcriptase to produce fluorophore-labeled RNA transcripts. RNA-based probes can be used to identify complementary sequences by<i> in situ</i> hybridisation to fixed cells and tissues by direct or indirect fluorescence detection or by membrane hybridisation procedures.
UOM: 1 * 250 Nmo
Product Image-ENZOENZ42809

Bio-N⁶-ddATP

Catalog Number: (ENZOENZ42809)
Supplier: ENZO LIFE SCIENCES
Description: Bio-N⁶-ddATP (Biotin-N⁶-(2’,3’-dideoxy)adenosine-5’ triphosphate) can be used for 3’-end labelling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-N⁶-ddAMP. It is also a substrate for Taq DNA Polymerase and other DNA polymerases.
UOM: 1 * 25 Nmo
Product Image-ENZOENZ42861

Allylamine-dUTP

Catalog Number: (ENZOENZ42861)
Supplier: ENZO LIFE SCIENCES
Description: Allylamine-2’-deoxyuridine-5’-triphosphate (Allylamine-dUTP) can replace TTP in reactions where it serves as a substrate for <i>E.coli</i> DNA polymerase 1 (holoenzyme and Klenow fragment), terminal deoxynucloetide transferase, T4 and Taq DNA polymerases, and reverse transcriptases (from ALV and MLV).
UOM: 1 * 1 Vial
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GFP-Certified™ FluoForte™ Reagent

Supplier: ENZO LIFE SCIENCES
Description: Calcium dye

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Reactive quencher dyes

Supplier: Biotium
Description: Reactive quenchers that act as acceptors in fluorescence resonance energy transfer (FRET) applications.

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Bilirubin conjugate, sodium salt

Supplier: Cayman Chemical
Description: Bilirubin conjugate is a stable, water-soluble ditaurate derivative of bilirubin meant to mimic endogenous bilirubin glucuronide derivatives.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
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