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You Searched For: Protein Extraction Reagents

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.


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Product Image-786-154

Detergent removal system, DetergentOUT™ GBS10

Supplier: G-Biosciences
Description: Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionisation when analysed by mass spectrometry.

Image Unavailable-786-214

Detergent removal system, DetergentOUT™ Tween®

Supplier: G-Biosciences
Description: DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.

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Product Image-786-090

Carrier proteins

Supplier: G-Biosciences
Description: The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.

Product Image-786-128

Detergent removal systems, OrgoSol DetergentOUT™

Supplier: G-Biosciences
Description: OrgoSol DetergentOUT™ is suitable for removal of detergents from protein solutions, including hydrophobic protein solutions. Compatible with all detergent types, its performance is not dependent on the concentration of detergents in the solution. It is highly flexible and can be used to process small and large sample volumes.

Product Image-PIER90051

Pierce™ EndoProteinase Lys-C Protease, MS Grade

Catalog Number: (PIER90051)
Supplier: Thermo Fisher Scientific
Description: Endoproteinase Lys-C protease is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.
UOM: 1 * 20 µG
Product Image-PIER20343

Immobilized pepsin, Pierce™

Catalog Number: (PIER20343)
Supplier: Thermo Fisher Scientific
Description: Immobilised Pepsin agarose consists of an acidic endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.
UOM: 1 * 5 mL
Product Image-PIER84841

Pierce™ Digestion Indicator for Mass Spectrometry, Thermo Scientific

Catalog Number: (PIER84841)
Supplier: Thermo Fisher Scientific
Description: The Digestion Indicator for Mass Spectrometry is a non-mammalian recombinant protein (26kDa) with five signature peptides for use in determining the digestion efficiency and reproducibility across multiple samples.
UOM: 1 * 10 µG
Product Image-786-428

Adenine deaminase

Catalog Number: (786-428)
Supplier: G-Biosciences
Description: Adenine deaminase converts adenine to hypoxanthine. It is a highly purified recombinant enzyme protein supplied at a 1 µM concentration.
UOM: 1 * 0,25 mL
Product Image-PIER88305

Detergent removal spin column kit, HiPPR™

Supplier: Thermo Fisher Scientific
Description: The HiPPR™ Detergent Removal Spin Column Kit improves mass spectrometry results by efficiently removing detergents from 25 to 200 µl samples with low protein or peptide concentrations.

Product Image-PIER87780

Pierce™ Detergent Removal Spin Columns and Resin

Supplier: Thermo Fisher Scientific
Description: Pierce™ Detergent removal resin enables removal of commonly used ionic, non-ionic and zwitterionic detergents from protein/peptide digest solutions.

Product Image-PIER20341

Immobilised papain, Pierce™

Catalog Number: (PIER20341)
Supplier: Thermo Fisher Scientific
Description: Pierce™ Immobilised Papain consists of a cysteine-endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab fragments from antibodies.
UOM: 1 * 5 mL
Product Image-ICNA116978025

Lysing matrix Z, MP Biomedicals

Supplier: MP Biomedicals
Description: Lysing Matrix Z is used primarily for lysis of animal and plant tissue, tougher stems, roots and seeds, and whole insects. Each impact-resistant tube contains yttria-stabilised zirconium oxide beads.

Product Image-786-791

Immobilised Pepsin

Catalog Number: (786-791)
Supplier: G-Biosciences
Description: A proteolytic enzyme immobilised on 4% agarose that is routinely used for the generation of F(ab’)₂ fragments from Immunoglobulin G (IgG). The pepsin has the ability to cleave the heavy chains near the hinge region. The immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.
UOM: 1 * 1 items
Product Image-786-790

Immobilised Papain

Catalog Number: (786-790)
Supplier: G-Biosciences
Description: A cysteine protease enzyme (EC 3.4.22.2) immobilised on 4% agarose, cleaves Immunoglobulin G (IgG) antibody molecules in the hinge region, generating three ~50 kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonisation, and lysis.
UOM: 1 * 1 items
Product Image-PIER20308

SDS precipitation kit, SDS-Out™

Catalog Number: (PIER20308)
Supplier: Thermo Fisher Scientific
Description: SDS-Out™ SDS Precipitation Kit provides convenient removal of SDS from small-volume (0,1 to 10 ml) protein samples.
UOM: 1 * 1 KIT
Product Image-PIER88216

Micrococcal nuclease solution (≥100 U/µl)

Catalog Number: (PIER88216)
Supplier: Thermo Fisher Scientific
Description: This Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.
UOM: 1 * 1 items
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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