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Product Image-AXYGMAG-DYECL-1

Axygen® AxyPrep® Mag DyeClean kit

Supplier: Corning
Description: Removing excess dye terminator is an essential step prior to Sanger sequencing. Carryover of the excess dye into the sequencing reactions may result in dye blobs resulting in inaccurate results. The AxyPrep™ Mag DyeClean kit utilises a paramagnetic bead-based purification system for Sanger sequencing reaction dye terminator clean-up. The protocol is simple and comprises binding, washing and elution steps which can be performed directly in the thermal cycling plate. This kit requires no centrifugation or filtration steps.

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Bacterial DNA kit, E.Z.N.A.®

Supplier: OMEGA BIO-TEK
Description: The E.Z.N.A.® bacterial DNA kit allows the rapid and reliable isolation of high quality total cellular DNA from a wide variety of bacterial species. This kit uses optimised lysis condition and up to 1×10⁹ bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or elution buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

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Product Image-D6904-04

E.Z.N.A.® Plasmid Midi kit

Supplier: OMEGA BIO-TEK
Description: E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200 µg of plasmid DNA in less than 60 minutes. This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. The DNase/RNase-free HiBind® DNA midi columns operate by eliminating time consuming phenol-chloroform extractions and alcohol precipitations. Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple bind-wash-elute procedure thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, <i>E.coli </i>strain, and conditions of growth, 50 ml of an overnight culture in LB medium will typically produce 200 µg of high-copy number plasmid DNA. When working with low-copy number plasmids the starting culture volume can be increased up to 100 ml. High-quality DNA is ready for immediate use in routine molecular biology applications, such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.

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Product Image-17-0998-01

Affinity chromatography media, Heparin Sepharose™ 6 Fast Flow

Catalog Number: (17-0998-01)
Supplier: Cytiva
Description: Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand. Heparin Sepharose 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.
UOM: 1 * 50 mL
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DNA isolation kit, EPIXTRACT®

Supplier: ENZO LIFE SCIENCES
Description: EPIXTRACT® DNA isolation kit provides a simple method to isolate DNA from plasma, serum, and body fluids.

Product Image-MMMABC1020

3M™ Polisher ST Laboratory Capsules

Catalog Number: (MMMABC1020)
Supplier: 3M
Description: 3M™ Polisher ST is a fully encapsulated anion exchange (AEX) product to reduce host sell protein impurities, viruses, and other negatively charged contaminants in flow-through polishing chromatography of biopharmaceutical process streams.
UOM: 1 * 1 items

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Product Image-PIER78115

Inclusion body solubilisation reagent, Pierce™

Catalog Number: (PIER78115)
Supplier: Thermo Fisher Scientific
Description: Inclusion Body Solubilisation Reagent is designed to retrieve expressed protein in soluble form after lysis and extraction with reagents.
UOM: 1 * 100 mL
Product Image-17-0529-01

Calmodulin Sepharose™ 4B Affinity Chromatography Media, Cytiva

Catalog Number: (17-0529-01)
Supplier: Cytiva
Description: Calmodulin Sepharose™ 4B is calmodulin immobilised by the CNBr method to Sepharose™ 4B. Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells. This protein is involved in many cellular processes such as glycogen metabolism, cytoskeletal control, neurotransmission, phosphate activity and control of NAD⁺/NADP⁺.
UOM: 1 * 10 mL

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Product Image-17-0489-01

Superose™ 6 Gel Filtration Media, Prep Grade

Catalog Number: (17-0489-01)
Supplier: Cytiva
Description: Superose™ 6 PG for separations of proteins and other biomolecules according to size. Wide separation in two molecular weight ranges from 1000 up to 5 million.
UOM: 1 * 125 mL
Product Image-17-5438-01

Affinity chromatography media, MabSelect SuRe™

Supplier: Cytiva
Description: MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.

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Product Image-17-0500-01

Ion exchange chromatography media, DEAE Sephacel™

Catalog Number: (17-0500-01)
Supplier: Cytiva
Description: DEAE Sephacel™ is a weak anion exchanger based on beaded cellulose. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range. This media is macroporous and has an exclusion limit of ~10000 kDa for globular proteins. Supplied pre-swollen in 20% ethanol.
UOM: 1 * 500 mL
Product Image-PIER21130

Streptavidin HRP conjugates, high sensitivity, Pierce™

Supplier: Thermo Fisher Scientific
Description: Pierce™ High Sensitivity Streptavidin-HRP is a peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

Product Image-17-5474-02

Affinity chromatography media, MabSelect SuRe™ LX

Supplier: Cytiva
Description: MabSelect SuRe™ LX is a protein A affinity medium with very high dynamic binding capacity at extended residence times and is developed for high titer antibody processes.

Product Image-17-0906-01

Affinity coupling media, NHS-Activated Sepharose™ 4 Fast Flow

Catalog Number: (17-0906-01)
Supplier: Cytiva
Description: NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.
UOM: 1 * 25 mL

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Product Image-ICNA111001400

DNA purification kits, GeneClean® II kit, MP Biomedicals

Catalog Number: (ICNA111001400)
Supplier: MP Biomedicals
Description: The GeneClean® II Kit is designed to optimally purify 200 bp to 20 kb DNA from TAE and TBE buffered agarose gels and solutions. The purified DNA is suitable for a variety of downstream applications, such as sequencing, PCR, restriction digestion, cloning and labeling.
UOM: 1 * 1 KIT
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Bacterial RNA kit, E.Z.N.A.®

Supplier: OMEGA BIO-TEK
Description: The E.Z.N.A.® Bacterial RNA kit is designed for isolation of high-quality total RNA from variety of bacterial strains. Up to 1×10⁹ log-phase bacterial cells can be processed. This kit uses an improved lysis procedure to ensure the complete lysis of bacterial cells. Purified RNA is suitable for downstream applications such as RT-PCR and hybridisation techniques.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
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