You Searched For: G Bioscienes

Catalog Number: (BE-301)

Supplier: G-Biosciences

Description: Bacterial conjugation is a naturally occurring process that allows the transfer of DNA from one bacterium to another, which allows the transfer of genetic traits, particularly drug resistance. The kit contains two bacteria with different drug resistance genes and all the tools for students to study bacterial conjugation. This kit teaches the difference between bacterial genomic DNA and the transferable plasmid DNA and the mechanisms of bacterial conjugation. Students will also learn important basic microbiological techniques, including bacterial growth in liquid broth and on solid agar plates, antibiotic selection of bacteria and important aseptic techniques.

UOM: 1 * 1 KIT

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Supplier: G-Biosciences

Description: Antibody Fab Fragmentation Kits are designed for the generation and isolation of Fab fragments from IgG molecules. The kits utilise Immobilised Papain resin. Immobilised Papain offers the advantage of generating Fab and fragments without the need to remove the papain enzyme after digestion. Following papain digestion the Fab fragments are separated from undigested IgG and the Fc region with the supplied Protein A Spin Column. Protein A Resin binds the IgG and Fc molecules and the Fab are rapidly collected.

Supplier: G-Biosciences

Description: Aqueous mixture of dATP, dCTP, dGTP and dTTP each at a final concentration of 10 or 25 mM.

Supplier: G-Biosciences

Description: The F(ab)₂ Fragmentation kits are designed for the generation and isolation of F(ab)₂ fragments from IgG molecules. The kit utilises Immobilised Pepsin resin. Pepsin is a proteolytic enzyme that is routinely used for the generation of F(ab)₂ fragments from immunoglobulin G (IgG). One or more of the disulfide bonds that join the heavy chains in the hinge region are preserved, so the two Fab regions of the antibody remain joined together, yielding a divalent molecule (containing two antibody binding sites), hence the designation F(ab)₂. The light chains remain intact and attached to the heavy chain, whereas the Fc fragment is digested into small peptides. The Immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.

Catalog Number: (786-11)

Supplier: G-Biosciences

Description: SG-Arginine-C™ endopeptidase (Clostripain, from <i>C. histolyticum</i>) specifically hydrolyses the carboxy peptide bond of arginine. SG-Arginine-C™ has been modified chemically by a propriety process to render the enzyme resistant to autolysis and stabilise enzymatic activity. In addition, as a sulfhydryl enzyme, SG-Arginine-C™ is susceptible to inactivation by oxidation and as a result requires reducing agents for protection. The enzyme also requires calcium ion for maximal activity. A special reconstitution buffer is supplied, which contains reducing agents and activators to maintain enzyme activity.

UOM: 1 * 2 items

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Catalog Number: (733-1945)

Supplier: G-Biosciences

Description: A ready to use, 100X concentrated, broad range protease inhibitor cocktail that is fully compatible with 2D electrophoresis and subsequent mass spectrometry.

UOM: 1 * 1 mL

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Supplier: G-Biosciences

Description: Agar

Supplier: G-Biosciences

Description: Nonidet® P 40 Substitute (NP-40) 10% in aqueous solution

Supplier: G-Biosciences

Description: N-Decyl-N,N-dimethyl-3-ammonio-1-propanesulphonate(sulphobetaine-10, SB-10)

Catalog Number: (DG009)

Supplier: G-Biosciences

Description: TRITON™ X-100 (Polyethylene glycol tert-octylphenyl ether)

UOM: 1 * 5 items

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Supplier: G-Biosciences

Description: NDSB 201 is a zwitterionic compound. Unlike zwitterionic detergents, the hydrophobic group in NDSB 201 is too short to form micelles, even at 1 M concentrations. NDSB 201 can be used for the purification of proteins and solubilisation of protein samples for 2D gel electrophoresis.

Catalog Number: (DG090)

Supplier: G-Biosciences

Description: Sodium deoxycholate

UOM: 1 * 100 g

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Supplier: G-Biosciences

Description: ProteCEASE™ is a dry format version of ProteaseArrest™ for large scale preparative applications and for those users who prefer reconstitution prior to use. ProteCEASE™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. EDTA is an optional component for inhibiting metalloproteases.

Catalog Number: (786-428)

Supplier: G-Biosciences

Description: Adenine deaminase converts adenine to hypoxanthine. It is a highly purified recombinant enzyme protein supplied at a 1 µM concentration.

UOM: 1 * 0,25 mL

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Supplier: G-Biosciences

Description: Running Buffer, Tris-Glycine native gel running buffer

Catalog Number: (786-R44)

Supplier: G-Biosciences

Description: Anti-IgG Goat Antibody (AP (Alkaline Phosphatase))

UOM: 1 * 1 mL

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