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Nunc™ EasYFlask™ Flasks with Nunclon™ Supra Surface

Supplier: Thermo Fisher Scientific
Description: Experience optimal performance with Thermo Scientific Nunc EasYFlask flasks with Nunclon Supra surface treatment for culturing a wide variety of fastidious adherent cells. The ergonomic design with angled neck and wide opening allows easy access to the growth surface by pipettes or cell scrapers. Intended for general cell culture applications, the Nunclon Supra surface is more hydrophilic than the Nunclon Delta surface, enhancing the growth of finicky cell types under no-serum or low-serum conditions.

Environmentally Preferable

Product Image-PIER88881

Cell-free protein expression kits, 1-step human coupled IVT kit - DNA

Supplier: Thermo Fisher Scientific
Description: The 1-Step Human Coupled IVT Kit – DNA is a mammalian<i> in vitro</i> translation (IVT) system based on HeLa cell lysates. The kit contains all of the cellular components required for protein synthesis, including ribosomes, initiation factors, elongation factors and tRNA. When supplemented with the included proprietary accessory proteins, reaction mix and a DNA template cloned into the Thermo Scientific pT7CFE1-based vector, this system can synthesise protein for up to 6 hours. The kit includes a pT7CFE-CHis vector for ease of use in downstream applications.

Product Image-M1682

Maxima™ H minus cDNA synthesis master mix, with dsDNase

Supplier: Thermo Fisher Scientific
Description: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix with dsDNase provides a simple workflow that combines genomic DNA elimination and cDNA synthesis in a one-tube procedure. The cDNA reaction components are pre-mixed into a complete master mix that is convenient to use, reduces pipetting steps, and is optimised for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. Maxima™ H Minus cDNA Synthesis Master Mix is optimised for reproducible cDNA synthesis at elevated temperatures (50 to 65°C) within 30 minutes.

Product Image-WITGDH.WHM12216

Heating mantles with reaction vessel, WiseTherm® WHM

Catalog Number: (WITGDH.WHM12216)
Supplier: witeg Labortechnik
Description: Heating mantles with aluminium exterior and fabric interior. The integrated K-type thermo-sensor is basically embedded (except fabric mantles) which enables precise control of the mantles when used together with remote digital controller. External thermo-sensor is available, which enables precise control of the temperature of liquid sample in the vessel when used together with remote digital controller.
UOM: 1 * 1 items
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SkanIt™ Upgrade package, version 5.0

Supplier: THERMO LABSYSTEMS LIFE SCIENCE
Description: This SkanIt Software upgrade package is specifically designed for Thermo Scientific Fluoroskan, Fluoroskan FL, and Luminoskan microplate readers to enable the use of SkanIt software and USB connection between instrument and PC. The upgrade package is also compatible with the discontinued Fluoroskan Ascent, Fluoroskan Ascent FL, and Luminoskan Ascent microplate readers. SkanIt Software is available in two editions: a research edition for scientists working in life science research and a drug discovery edition with features for compliancy with FDA 21 CFR Part 11. Note: the upgrade package is compatible with Ascent instruments that are equipped with internal software version 2.6.

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5µL Fixed Needle CTC/Thermo Syringe with 7.5cm 0.63mm OD Cone Tipped Needle PK25 1 * 25 items

Catalog Number: (SGEA001991)
Supplier: Trajan Scientific and Medical
Description: 5µL Fixed Needle CTC/Thermo Syringe with 7.5cm 0.63mm OD Cone Tipped Needle PK25 1 * 25 items
UOM: 1 * 25 items
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Direct PCR kits

Supplier: FINNZYMES REAGENTS
Description: Direct PCR Kits save time and cost by allowing amplification of DNA directly from the source material. Direct PCR is based on Thermo Scientific Phire® Hot Start II DNA Polymerase and Phusion® Hot Start II High-Fidelity DNA Polymerase. These fusion enzymes are exceptionally fast, robust and highly tolerant of many PCR inhibitors present in unpurified sample material. This allows reliable amplification in challenging conditions where conventional DNA polymerases are completely inhibited.

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EDC-HCl (N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride), No-Weigh™ Format, Pierce™

Catalog Number: (PIERA35391)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.
UOM: 1 * 10 mg
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EDC-HCl (N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride), Pierce™

Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.

MSDS Certificates

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dsDNase

Catalog Number: (EN0771)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific dsDNase is an engineered shrimp DNase designed for rapid and safe removal of contaminating genomic DNA from RNA samples. It is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. Highly specific activity towards double-stranded DNA ensures that RNA and single-stranded DNA, such as cDNA and primers are not cleaved. dsDNase is easily inactivated by moderate heat treatment (55 °C). These features make dsDNase an excellent choice for gDNA elimination prior reverse transcription. It allows for dramatically simplified workflow which combines genomic DNA elimination and cDNA synthesis into one-tube procedure.
UOM: 1 * 1 items
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Knitted Mittens, Polysafe®, MTK / 40 / DKL

Catalog Number: (112-2296)
Supplier: Bennett Safetywear
Description: Very cut-resistant and thermo-insulating terry toweling mittens with 100% Kelvar®. The lining is made of flame retardant cotton. Ideal when handling heavy, hot parts.
UOM: 1 * 1 PAIR
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Coomassie® brilliant blue R-250

Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce coomassie brilliant blue dyes are composed of one of the most common forms of coomassie dye, which is a key component of various colorimetric protein gel stains. Coomassie R-250 and G-250 dyes are two chemical forms of a disulphonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and bradford-type assay reagents for protein quantitation. The R-250 (red-tinted) form lacks two methyl groups that are present in the G-250 (green-tinted) form, which is also called colloidal coomassie dye.

Certificates

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VWR® Q Path® MicroStar II, Cassettes, in Sleeve

Supplier: VWR Collection
Description: Acetal polymer. Suitable for holding and identifying tissue samples in processing, embedding and sectioning procedures. Resistant to all commonly used histological solvents. Cassettes in pre-filled sleeves for Thermo and RA Lamb cassette printers. 50 dispensers with 80 cassettes and 2×2000 lids.

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VWR® Q Path® MacroStar II, Cassettes, in Sleeve

Supplier: VWR Collection
Description: Acetal polymer. Suitable for holding and identifying tissue samples in processing, embedding and sectioning procedures. Resistant to all commonly used histological solvents. Cassettes in pre-filled sleeves for Thermo and RA Lamb cassette printers. 50 dispensers with 80 cassettes and 2×2000 lids.

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Protease inhibitor cocktail, Halt

Supplier: Thermo Fisher Scientific
Description: Halt Protease Inhibitor Cocktail provides all the protection of a broad-spectrum protease inhibitor in a single, ready-to-use liquid format. Halt protects against protein degradation by serine, cysteine, and aspartic acid proteases and aminopeptidases. Metalloproteases are inhibited by the optional use of 0,5 M EDTA. The 100X Halt stock solution makes it easy to dispense the required amount (10 µl per 1 ml) of protease inhibitors into buffers or lysis reagents prior to extraction of proteins from cultured cells, animal tissues, plant tissues, yeast or bacteria. Halt Protease Inhibitor Cocktails are compatible with Thermo Scientific Pierce Protein Extraction Reagents. Halt EDTA-free formulation is ideal for preparing samples that will be analysed by 2-D gel electrophoresis, immobilised metal chelate affinity chromatography (IMAC) and other downstream application where chelating agents interfere.

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Glass screw top vials, 2 ml, level 2 high-throughput applications, SureSTART™

Supplier: Thermo Fisher Scientific
Description: Choose Thermo Scientific™ SureSTART™ 2 ml glass screw top vials, performance level 2, to ensure high quality data with an uninterrupted workflow in high-throughput applications using GC, HPLC/UHPLC, and single or triple quadrupole MS systems. Use level 2 vials for routine QA/QC applications, where the analyte levels are achievable or close to the method detection level. Available in clear and amber glass, these 2 ml screw top vials have very low metal content, extractables, and leachables and conform to high standards so that you can to meet your regulatory requirements. They are specification certified to ensure their physical dimensions and autosampler compatibility.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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