You Searched For: Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

Description: GeneJET™ Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from a wide variety of plant species and tissue types.

Supplier: Thermo Fisher Scientific

Description: The Maxima™ SYBR® Green/Fluorescein qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase and dNTPs in an optimised PCR buffer. It contains SYBR® Green I dye and is supplemented with fluorescein passive reference dye. Fluorescein is included into the master mix to serve as an internal reference for normalisation
of the SYBR Green I fluorescent signal between different wells to compensate for any instrument or pipetting variation. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG)*. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. The use of Maxima™ SYBR® Green/Fluorescein qPCR Master Mix in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Catalog Number: (SM1103)

Supplier: Thermo Fisher Scientific

Description: FastRuler ultra low range DNA ladder is specifically designed for fast sizing and approximate quantification of double-stranded DNA in 48 well (or 96-well) high throughput gels, as well as in conventional agarose gels. The ladder is a mixture of five blunt-ended chromatography-purified individual DNA fragments that are easily resolved in a short separation distance (10 to 20 mm) after an 8 to 14 minutes run on the appropriate agarose gel.

UOM: 1 * 1 KIT

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Catalog Number: (SM0383)

Supplier: Thermo Fisher Scientific

Description: MassRuler Low Range DNA Ladder, ready to use, 80 to 1031 bp, is specially designed for accurate quantification and sizing of DNA fragments by agarose gel electrophoresis.

UOM: 1 * 1 KIT

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Supplier: Thermo Fisher Scientific

Description: Catalyses the transfer of the γ-phosphate from ATP to the 5'-hydroxyl group of single- and double-stranded DNAs and RNAs, oligonucleotides, or nucleoside 3'-monophosphates (forward reaction).

Catalog Number: (EN0525)

Supplier: Thermo Fisher Scientific

Description: DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyses phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'- phosphate and 3'- OH groups.

UOM: 1 * 1 KIT

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Supplier: Thermo Fisher Scientific

Description: RevertAid™ H Minus Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to point mutation in the RNase H domain. RevertAid™ H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full-length cDNA from long templates are obtained. RevertAid™ H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 55 °C), which makes the enzyme an ideal tool for reverse transcription of RNAs having a high degree of secondary structure.

Supplier: Thermo Fisher Scientific

Description: GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.

Catalog Number: (R0861)

Supplier: Thermo Fisher Scientific

Description: Dithiothreitol (DTT, Cleland's reagent)

UOM: 1 * 5 g

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Catalog Number: (ER1751)

Supplier: Thermo Fisher Scientific

Description: CCNGGA sites and cuts best at 37 °C in Tango buffer.

UOM: 1 * 1 KIT

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Supplier: Thermo Fisher Scientific

Description: Maxima™ Reverse Transcriptase is an advanced enzyme derived by <i>in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR. The Maxima™ Reverse Transcriptase is capable of cDNA synthesis at elevated temperatures (50 to 60 °C) and completion of the reverse transcription reaction in 15 to 30 minutes. The use of this enzyme in combination with Maxima™ qPCR Master Mixes ensures high specificity, sensitivity and wide linear range of two step RT-qPCR. Applications include first strand cDNA synthesis, RT-PCR, and RT-qPCR.

Supplier: Thermo Fisher Scientific

Description: RevertAid™ H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid™ H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

Supplier: Thermo Fisher Scientific

Description: The IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose

Supplier: Thermo Fisher Scientific

Description: O'RangeRuler™ DNA ladders are step ladders that consist of basic unit repeats of 10, 15, 20, 50, 100, 200, or 500 bp. The ladders have a higher density of bands at a specific DNA size range and are therefore suitable for more accurate sizing of DNA in agarose or non denaturing polyacrylamide gels.

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.

Supplier: Thermo Fisher Scientific

Description: Oligo(dT)₁₈ primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'-hydroxyl ends. The primer is supplied as a ready to use, 20X concentrated aqueous solution. Oligo(dT)₁₈ primer has been functionally tested in first strand cDNA synthesis.