You Searched For: Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

Description: RevertAid™ Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids, which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Catalog Number: (FD1464)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

UOM: 1 * 1 KIT

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Supplier: Thermo Fisher Scientific

Description: The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host <i>E. coli</i> cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

Catalog Number: (ER1921)

Supplier: Thermo Fisher Scientific

Description: CCWGG sites and cuts best at 37 °C in O buffer.

UOM: 1 * 1 KIT

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Catalog Number: (EO0461)

Supplier: Thermo Fisher Scientific

Description: Agarase is an enzyme that specifically digests the agarose polysaccharide core into neoagaro-oligosaccharides.

UOM: 1 * 1 KIT

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Catalog Number: (ER0303)

Supplier: Thermo Fisher Scientific

Description: ATC sites and cuts best at 37 °C in R buffer Isoschizomers: EcoRV.

UOM: 1 * 1 KIT

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Supplier: Thermo Fisher Scientific

Description: Thermo Scientific 5-Fluoroorotic Acid (5-fluorouracil-6-carboxylic acid monohydrate; 5-FOA) is used in yeast molecular genetics to detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase

Catalog Number: (R0862)

Supplier: Thermo Fisher Scientific

Description: Dithiothreitol (DTT, Cleland's reagent)

UOM: 1 * 1 KIT

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Supplier: Thermo Fisher Scientific

Description: Exonuclease I (ExoI) degrades single-stranded DNA in a 3' to 5'direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.

Catalog Number: (ER0053)

Supplier: Thermo Fisher Scientific

Description: GATCC sites and cuts best at 37 °C in its own unique buffer.

UOM: 1 * 1 KIT

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Catalog Number: (FD0974)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

UOM: 1 * 1 KIT

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Catalog Number: (FD1254)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

UOM: 1 * 1 KIT

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Supplier: Thermo Fisher Scientific

Description: Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mix is a ready to use 2X reaction mix that includes DreamTaq™ Hot Start DNA Polymerase, DreamTaq™ buffer, magnesium, and dNTPs. The master mix allows easy reaction setup with fewer pipetting steps, with only primers, template, and water needing to be added. The green master mix includes density reagent and two tracking dyes for convenient direct loading of PCR products on a gel.

Supplier: Thermo Fisher Scientific

Description: The Maxima™ SYBR® Green/ROX qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start <i>Taq</i> DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer and is supplemented with ROX passive reference dye. Only template and primers need to be added. Maxima™ hot start <i>Taq</i> DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green/ROX qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

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Supplier: Thermo Fisher Scientific

Description: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Catalog Number: (EM0821)

Supplier: Thermo Fisher Scientific

Description: CpG methyltransferase enzyme methylates the C5 position on the base moiety of all cytosine nucleotides contained in unmethylated or hemimethylated double stranded DNA in a 5'-CpG-3' context. Thermo Scientific M.SssI is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes modification of all CpGs in 15 minutes at 37 °C. In addition, the enzyme has been specifically validated for use with genomic DNA – the primary substrate in epigenetic studies.

UOM: 1 * 1 KIT

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