You Searched For: G Bioscienes

Catalog Number: (786-651)

Supplier: G-Biosciences

Description: β-Galactosidase assay is for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. It uses chlorophenol red-β-D-galactopyranoside (CPRG), which is up to 10 times more sensitive than the classic O-nitrophenyl-β-D-galactopyranoside (ONPG) β-Galactosidase assays. This increased sensitivity makes it simpler to measure β-galactosidase activity in cells that are difficult to transfect or have low β-galactosidase activity expression. β-Galactosidase activity is measured using a spectrophotometer or a microplate reader to determine the amount of substrate converted at 570 to 595 nm.

UOM: 1 * 500 Assays

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Supplier: G-Biosciences

Description: Protein assay, Bovine γ-Globulin Protein Standard (2 mg/ml)

Catalog Number: (786-627)

Supplier: G-Biosciences

Description: For improved dialysis time and rates.

UOM: 1 * 50 mL

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Supplier: G-Biosciences

Description: Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of albumin from such samples.

Supplier: G-Biosciences

Description: GET™ CLEAN DNA uses high binding affinity GET™ spin columns to remove salts, enzymes, unincorporated nucleotides, radiolabels, and primer-dimers from any DNA preparation of 100 bp to 20 kb. GET™ spin columns has an enhanced binding affinity for DNA, thus eliminating loss or damage of DNA.

Catalog Number: (786-791)

Supplier: G-Biosciences

Description: A proteolytic enzyme immobilised on 4% agarose that is routinely used for the generation of F(ab’)₂ fragments from Immunoglobulin G (IgG). The pepsin has the ability to cleave the heavy chains near the hinge region. The immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.

UOM: 1 * 1 items

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Catalog Number: (786-790)

Supplier: G-Biosciences

Description: A cysteine protease enzyme (EC 3.4.22.2) immobilised on 4% agarose, cleaves Immunoglobulin G (IgG) antibody molecules in the hinge region, generating three ~50 kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonisation, and lysis.

UOM: 1 * 1 items

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Catalog Number: (786-680)

Supplier: G-Biosciences

Description: Protein-OUT™ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUT™ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUT™ removes inadvertent contamination with dust and keratin.

UOM: 1 * 50 mL

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Catalog Number: (786-678)

Supplier: G-Biosciences

Description: Swift™ Film Cleaner allows researchers to clean film that has been overexposed or has a high background or speckling without having to repeat experiments. Suitable for all exposed film developed for gel shift assays, Western, Southern and Northern blots.

UOM: 1 * 1 KIT

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Catalog Number: (786-453)

Supplier: G-Biosciences

Description: The Phosphatase Assay Kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases. The Phosphatase Assay Kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases.

UOM: 1 * 1 KIT

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Supplier: G-Biosciences

Description: Perfect-FOCUS™ is based on quantitative precipitation and concentration of protein solutions using UPPA™ (Universal Protein Precipitation Agent) and has been specifically developed for preparing lower conductivity protein samples for iso-electric focusing gels. Perfect-FOCUS™ is designed to clean and remove interfering agents from protein samples and concentrate protein solutions that give poor protein spot resolution during 2D electrophoresis.

Catalog Number: (786-488)

Supplier: G-Biosciences

Description: Potassium dihydrogen phosphate 1 mol/l in aqueous solution

UOM: 1 * 1 L

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Catalog Number: (786-258)

Supplier: G-Biosciences

Description: Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilises nearly all of the proteins from <i>E.coli</i>, including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme enzyme, followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.

UOM: 1 * 1 KIT

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Catalog Number: (R013)

Supplier: G-Biosciences

Description: A DNase and RNase free solution of 0.5M EDTA.

UOM: 1 * 100 mL

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Catalog Number: (RCH-001)

Supplier: G-Biosciences

Description: Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.

UOM: 1 * 25 AU

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Supplier: G-Biosciences

Description: Tris is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane, with the formula (HOCH2)3CNH2. Tris is extensively used in as a major component in buffer solutions.