You Searched For: G Bioscienes

Catalog Number: (786-019)

Supplier: G-Biosciences

Description: Efficient™ Western Transfer Buffer (20X) achieves increased protein transfer efficiency. This ready-to-use transfer buffer is prepared for optimal conductivity and efficient protein transfer, without generating excessive heat or transfer distortion.

UOM: 1 * 1 L

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Supplier: G-Biosciences

Description: Prepacked purification columns for protein purification. Supplied in packs of five.

Catalog Number: (BE-416)

Supplier: G-Biosciences

Description: The Hydrophobic Chromatography kit is designed to teach students the basic principle of hydrophobic chromatography utilising a hydrophobic enzyme. The use of the enzyme allows purification followed by a simple enzyme assay to detect the fractions that contain the enzyme. This lab activity involves preparation of a crude protein extract and running hydrophobic chromatography to isolate the enzyme.

UOM: 1 * 1 KIT

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Catalog Number: (BE-603)

Supplier: G-Biosciences

Description: Dialysis is a routinely used technique in research laboratories to 'change' the solution a biomolecule is dissolved in. Often the buffers used to isolate biomolecules, such as proteins, are not compatible with downstream applications due to high concentrations of reagents, such as salts. A sample is placed in a bag with a semi-permeable membrane and then placed in the new solution or water. Small molecules, such as salt, can pass through the membrane, moving from an area of high concentration to low concentration. The larger molecules, such as proteins, are retained in the bag. Microdialysis is a modification of dialysis to overcome the problem of dialysing small volumes of precious samples. Microdialysis uses small devices designed for small volumes. This kit allows students to study dialysis using patented microdialysis devices.

UOM: 1 * 1 KIT

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Catalog Number: (BE-406)

Supplier: G-Biosciences

Description: Using the Protein Electrophoresis kit, students will learn the principles of various types of electrophoresis, including denaturing and non-denaturing electrophoresis, and how this powerful technique is used to analyse proteins. The kit will introduce students to the different separation matrices currently in use and will understand their differing separation properties and their role in protein analysis. Students have an option of casting their own electrophoresis gels using polyacrylamide or using pre-cast commercially available gels. This kit is provided with all of the reagents, buffers and supplies needed for casting acrylamide gels, preparing protein samples, running electrophoresis, and staining the gels for visualisation of protein bands. Test protein samples and protein standards are also provided.

UOM: 1 * 1 KIT

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Catalog Number: (BE-409)

Supplier: G-Biosciences

Description: In this lab activity, students learn to perform carefully controlled experiments to generate protein fragments using a proteolysis enzyme and then analyse the protein fragments by electrophoresis. By analysis of protein fragmentation patterns, i.e. protein fingerprints, students learn about protein sequence, structure, and their conservation. Students resolve a set of three functionally identical protein samples selected from throughout the animal kingdom; including human, bovine and sheep. After generating fingerprints by electrophoresis, students examine the protein fingerprint of each sample to determine the degree of conservation.

UOM: 1 * 1 KIT

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Catalog Number: (RC-054)

Supplier: G-Biosciences

Description: Glycine

UOM: 1 * 500 g

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Catalog Number: (DG013)

Supplier: G-Biosciences

Description: Tween® 80 (Polysorbate) 10% in aqueous solution

UOM: 1 * 5 items

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Supplier: G-Biosciences

Description: AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and change colour in response to the chemical reduction of growth medium due to cell growth.

Supplier: G-Biosciences

Description: These C18 spin columns are ready-to-use micro centrifuge columns for peptide clean up and concentration. The columns consist of porous C18 reverse-phase resin that has a particle size of ~15 µm and a pore size of 300 Å. The resin offers highly efficient binding and recovery of peptides and is ideal for mass spectrometry and other peptide related applications.

Supplier: G-Biosciences

Description: Nourseothricin sulphate is a Streptothricin-class antibiotic. It has an extremely broad spectrum of activity against viruses, bacteria, eukaryotic unicellular and complex organisms. Norseothricin contains both Streptothricin D (C₃₁H₅₈N₁₂O₁₀) and Streptothricin F (C₁₉H₃₄N₈O₈) components.

Supplier: G-Biosciences

Description: Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.

Catalog Number: (786-360)

Supplier: G-Biosciences

Description: Extracts and solubilises nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubilise even the most difficult proteins.

UOM: 1 * 1 KIT

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Catalog Number: (BC87)

Supplier: G-Biosciences

Description: Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow colored product at 412nm.
Ellman's reagent is very useful as a free sulfhydryl assay reagent due to its high specificity for -SH groups at neutral pH, high molar extinction coefficient and short reaction time.

UOM: 1 * 5 g

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Catalog Number: (BB01)

Supplier: G-Biosciences

Description: G-Biosciences' Botanical Garden Blot is prepared using total protein extracted from a variety of 5 to 10 day old, whole plant tissues.

UOM: 1 * 1 items

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Supplier: G-Biosciences

Description: EDC is a heterobifunctional, water-soluble, zero-length carbodiimide crosslinker that is used to couple carboxyl groups to primary amines. EDC activates carboxyl groups first and forms amine reactive O-acylisourea imtermediate that spontaneously reacts with primary amines to form an amide bond and isourea by-product.