You Searched For: G Bioscienes

Catalog Number: (BE-203)

Supplier: G-Biosciences

Description: Antibiotics play a crucial role in the manipulation, screening and killing of bacteria in a range of biotechnology processes. This kit specifically teaches the basic principles of antibiotics, bacterial resistance and susceptibility. Students learn and understand the use of antibiotic resistance in screening for infectious diseases. Utilising a bacterial strain, students learn the effects of different antibiotics and visualise bacterial sensitivity and resistance to the supplied antibiotics. This method involves the use of filter paper discs impregnated with a specified concentration of antibiotics on the surface of an agar plate containing microbial cells. This kit will enable students to analyse the inhibitory effects of different antibiotics on selected bacterial cells and then determine which antibiotic is the most suitable to treat a bacterial infection.

UOM: 1 * 1 KIT

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Catalog Number: (BE-507)

Supplier: G-Biosciences

Description: A hands on lab activity to study immunoaffinity chromatography and use a specific antibody to purify antigenic proteins from complex samples. This technique involves performing a chromatography procedure using antigen or antibody immobilised on a chromatographic resin. The solution containing antigen or antibody is passed through the column, which specifically and efficiently captures antibodies (antigen). The captured molecules are eluted from the column as a pure fraction. In this lab activity, students perform immunoaffinity chromatography and learn how this method is utilised in research laboratories.

UOM: 1 * 1 KIT

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Catalog Number: (786-802)

Supplier: G-Biosciences

Description: The Pearl™ Monoclonal IgG Purification Kit allows for the rapid purification of antibodies from cell culture supernatant and ascites fluid. The Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The IgG molecules can be stored or used in downstream applications without further clean-up, such as ammonium sulphate precipitation.

UOM: 1 * 1 KIT

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Catalog Number: (RC-017)

Supplier: G-Biosciences

Description: Ammonium peroxodisulphate (APS), powder for electrophoresis

UOM: 1 * 100 g

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Catalog Number: (GENO786-422)

Supplier: G-Biosciences

Description: Acetate film with acrylic adhesive. This self-adhesive microplate sealing tape is suitable for sealing both 96-well and 384-well plates. The precut, pressure-senstive tape seals and reseals the surface of the plates during incubations to prevent evaporation and allow more vigorous mixing. The tape will reseal the plates 2 to 3 times.

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Supplier: G-Biosciences

Description: X-gal (5-bromo-4-chloro-3-indolyl-β-D- galactopyranoside) is used to indicate whether bacteria express the ß-galactosidase enzyme, which is encoded by the lacZ gene. X-gal is cleaved by ß-galactosidase yielding galactose and 5-bromo-4-chloro-3-hydroxyindole, which is oxidised into 5,5'-dibromo-4,4'-dichloro-indigo, an insoluble blue product. Thus, if X-gal and an inducer of ß-galactosidase (usually IPTG) is contained within an agar medium on a culture plate, colonies which have a functional lacZ gene can easily be distinguished by their blue colour.

Catalog Number: (786-180)

Supplier: G-Biosciences

Description: Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.

UOM: 1 * 500 mL

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Catalog Number: (786-253)

Supplier: G-Biosciences

Description: FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins.

UOM: 1 * 1 KIT

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Catalog Number: (786-248)

Supplier: G-Biosciences

Description: FOCUS™ Cytoplasmic and Nuclear Protein Fractionation Kit for the fractionation of nuclear and cytoplasmic proteins from cells and tissues.

UOM: 1 * 1 KIT

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Catalog Number: (BC92)

Supplier: G-Biosciences

Description: This sulpho-SHPP (water-soluble Bolton-Hunter Reagent) is designed to aid the labelling of proteins with radioactive iodine. Radioactive iodine (¹²⁵I) is routinely used by researchers to label proteins. The iodination of proteins can be performed enzymatically or chemically.

UOM: 1 * 100 mg

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Catalog Number: (RC-002)

Supplier: G-Biosciences

Description: Acrylamide

UOM: 1 * 500 g

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Catalog Number: (DG064)

Supplier: G-Biosciences

Description: A Zwitterionic aminosulfobetaine with an aromatic core that stabilizes and solubilizes integral membrane proteins.

UOM: 1 * 1 g

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Catalog Number: (786-163)

Supplier: G-Biosciences

Description: Enhancer™ dot blot system allows for the rapid application of up to 384 protein or nucleic acid samples on to a 12×9 cm membrane for subsequent analysis. Samples are applied to a nitrocellulose or nylon membrane by capillary action, resulting in concentration of sample due to minimal diffusion.

UOM: 1 * 1 KIT

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Supplier: G-Biosciences

Description: A single step stain for the rapid detection of proteins resolved by PAGE. The stain is based on the interaction of zinc ions with polyacrylamide and proteins.

Catalog Number: (786-045)

Supplier: G-Biosciences

Description: Antipain hydrochloride inhibits Ca2+-dependent endopeptidases, including papain, trypsin-like serine proteases, some cysteine proteases and to a lesser extent plasmin. It has a higher specificity for trypsin and papain compared to leupeptin.

UOM: 1 * 5 mg

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Catalog Number: (786-247)

Supplier: G-Biosciences

Description: FOCUS™ Soluble & Insoluble is a complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a strong chaotropic extraction buffer to solubilise difficult proteins.

UOM: 1 * 1 KIT

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