You Searched For: Thermo Fisher Scientific

Catalog Number: (PIERA35394)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

UOM: 1 * 10 mg

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Catalog Number: (PIERA35349)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce TCEP-HCl is a potent, versatile, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds. The unique compound is easily soluble and very stable in many aqueous solutions. TCEP reduces disulfide bonds as effectively as dithiothreitol (DTT), but unlike DTT and other thiol-containing reducing agents, TCEP does not have to be removed before certain sulfhydryl-reactive crosslinking reactions.

UOM: 1 * 10 mg

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Catalog Number: (PIERA39268)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce Sulfo-SMCC, No-Weigh Format is a water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 angstroms).

UOM: 1 * 20 mg

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Supplier: Thermo Fisher Scientific

Description: SulfoLink™ Immobilisation Kit for Proteins is a kit containing all the reagents for preparing the protein sample for efficient immobilisation and columns containing SulfoLink™ resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.

Supplier: Thermo Fisher Scientific

Description: Pierce™ High capacity streptavidin agarose consists of purified recombinant streptavidin that has been covalently immobilised at a high density onto high-quality crosslinked 6% beaded agarose.

Supplier: Thermo Fisher Scientific

Description: Pierce™ Protein A chromatography cartridges are convenient, ready to use pre-packed devices for the isolation and purification of polyclonal and monoclonal antibodies. These cartridges are effective for routine affinity purification of polyclonal and monoclonal IgG from serum, ascites and cell culture supernatant.

Supplier: Thermo Fisher Scientific

Description: Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions.

Catalog Number: (PIER24600)

Supplier: Thermo Fisher Scientific

Description: Pierce silver stain for mass spectrometry is a complete kit for rapid and ultra-sensitive silver staining of proteins in polyacrylamide gels and efficient destaining of excised gel pieces for mass spectrometry analysis. This kit enables both first-time and experienced users to achieve consistent and reliable staining using high, low, and gradient-percentage gels in 1D and 2D formats. The optimised staining method ensures extremely sensitive staining while minimizsing covalent crosslinking of protein to the gel matrix, which can inhibit protein recovery. The destaining reagents facilitate complete removal of silver from stained protein bands and maximum protein recovery for subsequent mass spectrometry analysis.

UOM: 1 * 1 KIT

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Supplier: Thermo Fisher Scientific

Description: The Pierce™ Mouse IgG1 Fab and F(ab')2 Preparation Kits use immobilised ficin protease to efficiently digest mouse IgG1 into Fab or F(ab')2 fragments.

Catalog Number: (PIER78434)

Supplier: Thermo Fisher Scientific

Description: E-64 Protease Inhibitor

UOM: 1 * 10 mg

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Catalog Number: (PIER44688)

Supplier: Thermo Fisher Scientific

Description: Pierce™ F(ab')2 micro preparation kit enables efficient Fab generation from IgG.

UOM: 1 * 1 KIT

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Catalog Number: (PIER53029)

Supplier: Thermo Fisher Scientific

Description: Pierce™ NHS-Fluorescein antibody labeling kit is a multi-component kit containing a high-performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

UOM: 1 * 1 KIT

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Catalog Number: (PIER22858)

Supplier: Thermo Fisher Scientific

Description: Pierce™ Dye Removal Columns enable fast and efficient removal of non-reacted fluorescent dyes from protein labeling reactions.

UOM: 1 * 10 items

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Supplier: Thermo Fisher Scientific

Description: GelCode blue safe protein stain is a fast and sensitive coomassie-dye reagent that provides one-step ultrasensitive detection of proteins in polyacrylamide gels and is formulated to avoid hazardous shipping.

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Catalog Number: (PIER69702)

Supplier: Thermo Fisher Scientific

Description: Pierce™ cellulose acetate filter spin cups are convenient tools for manipulating small volumes of affinity supports (20 - 400 µl) for protein purification. Simply add the affinity resin and sample to one of the spin cups, then use a microcentrifuge to efficiently wash away contaminants and elute the purified sample without losing any resin in the process. Spin cups allow more protein to be affinity purified in less time.

UOM: 1 * 50 items

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Catalog Number: (PIER90054)

Supplier: Thermo Fisher Scientific

Description: Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.

UOM: 1 * 50 µG

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