You Searched For: Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

Description: Photoactivatable Biotin, Biotin-LC-ASA , Psoralen-PEG3-Biotin and TFPA-PEG3-Biotin each contain a photoactivatable group. When exposed to UV light, they become activated and insert non specifically into nearby molecules. These reagents may be used to label proteins and peptides, but they are also useful in labeling DNA, RNA and other molecules that do not contain any readily reactive functional groups. Psoralen-PEG3-Biotin contains a reactive psoralen group and is
designed to efficiently label nucleic acids. The psoralen group intercalates into the helix of DNA, allowing it to label efficiently and selectively. The psoralen can also stack along with the bases of single-stranded DNA or RNA increasing labeling efficiency and selectivity.

Catalog Number: (PIER78436)

Supplier: Thermo Fisher Scientific

Description: Pepstatin A Protease Inhibitor

UOM: 1 * 25 mg

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Supplier: Thermo Fisher Scientific

Description: PP column, PE filter, ~30 µm pore size. Pierce™ centrifuge columns are made from low protein binding polypropylene for compatibility with protein purification, and fit into standard centrifuge tubes for use in any centrifuge (1,5, 2, 15 and 50 ml).

Supplier: Thermo Fisher Scientific

Description: AminoLink™ Plus Coupling Resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines to prepare columns for affinity purification.

Supplier: Thermo Fisher Scientific

Description: The NHS-Azide and NHS-Phosphine Reagents are amine-reactive compounds for derivatising primary amines of proteins or amine-coated polymer surfaces. Once a protein or surface is azide- or phosphine-labelled, the two components are mixed for effective and stable conjugation. Phosphine groups react with azides via a Staudinger reaction to produce an aza-ylide intermediate that is trapped to form a stable, covalent amide bond. Because phosphines and azides are absent from biological systems, there is minimal background labelling of macromolecules found in cells or lysates.

Catalog Number: (PIER28355)

Supplier: Thermo Fisher Scientific

Description: Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

UOM: 1 * 1 items

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Supplier: Thermo Fisher Scientific

Description: These Western blotting filter papers are pre-cut cotton sheets for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels (SDS-PAGE) to PVDF, nitrocellulose, or other membranes.

Catalog Number: (PIER88901)

Supplier: Thermo Fisher Scientific

Description: EZ-Link® Phosphine-PEG3-Biotin is a versatile biotinylation reagent for labeling azide-containing molecules, which enables biotin-based detection and affinity purification of molecules via Staudinger ligation strategies.

UOM: 1 * 10 mg

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Catalog Number: (PIER20158)

Supplier: Thermo Fisher Scientific

Description: LightShift™ Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).

UOM: 1 * 1 items

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Supplier: Thermo Fisher Scientific

Description: The Pierce™ ECL Plus Western Blotting Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

Catalog Number: (PIER88302)

Supplier: Thermo Fisher Scientific

Description: Pierce™ Graphite spin columns improve phosphopeptide analysis by efficiently binding hydrophilic peptides and efficiently removing urea, salts and other contaminants before mass spectrometry analysis.

UOM: 1 * 1 KIT

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Supplier: Thermo Fisher Scientific

Description: Pierce™ Glutathione chromatography cartridges are convenient, ready to use pre-packed devices for the purification of GST-fusion proteins from cellular lysates. The glutathione is immobilised through its central sulfhydryl onto 6% crosslinked agarose resin. Purification of GST-fusion proteins using glutathione (GSH) agarose beads is well documented and provides a one-step, high purity affinity purification.

Supplier: Thermo Fisher Scientific

Description: Carboxyl groups, in the form of carboxy termini, aspartate residues or glutamate residues, can be targeted for biotin labeling using amine-derivatised biotin molecules. This reaction is mediated by a class of crosslinkers known as carbodiimides and results in the formation of an amide bond. The reaction with EDC, the most common carbodiimide crosslinker, is generally performed in an MES buffer at pH 4,5 to 5 and requires just minutes to complete.

Supplier: Thermo Fisher Scientific

Description: The Pierce™ NHS-activated magnetic beads enable covalent, amine-based conjugation of proteins to magnetic beads in a simple mix-and-go format for use in custom affinity purification experiments.

Supplier: Thermo Fisher Scientific

Description: This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.

Catalog Number: (PIER88303)

Supplier: Thermo Fisher Scientific

Description: Phosphopeptide enrichment spin tips with TiO₂ resin, 96 tips, Pierce

UOM: 1 * 1 KIT

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